RGC axon terminals in albino mice. A) Serial vibratome sections through the left and right dLGNs of an albino mouse. From top to bottom section progress rostral to caudal. CtB labeling of RGCs from the left (magenta) and right (green) eyes. White arrowheads indicate island. B) Single confocal section through CtB labeled albino island. Left shows background fluorescence (blue) and absence of ipsilateral axons (red). Right shows CtB labeled RGC boutons from contralateral eye only. Inset shows enlarged view of RGC boutons. C) Electron micrograph of retinogeniculate glomerulus in the albino island. D) Electron micrograph of non-glomerular neuropil of the exclusion zone. Red circles indicate non-glomerular synapses.

Retinal innervation of TCs in the albino dLGN. Dotted oval indicates the boundary of the RGC island in multiple panels. Four cells (TC1, TC2, TC3, TC9) are identified in panel B, G, H, and I. Tissue orientation is indicated by compass rose or rotation cube (Rostral/Medial/Ventral). A) Matching of optical images and EM section overview. Red rectangles indicate the boundaries of the EM volume used to trace TCs. White circles indicate blood vessels matched between optical images and EM images. B) Closer view of light and EM images showing nuclei of EM-reconstructed TCs. In the optical image (left), DAPI labeled nuclei (blue) of reconstructed TCsare outlined (cyan) relative to RGC boutons (green). The position of reconstructed TC that are not visible in the confocal image stack are indicated by circles. The nuclei labels are mapped onto the EM section (right). The nuclei of reconstructed TCs visible in the EM section are highlighted in red. C) Example of electron micrograph with 20 nm pixel size in which TC dendrite and associated glomerulus are annotated. D) EM reconstructed island (green) and non-island (magenta) RGC boutons that were found innervating reconstructed TCs. EM boutons are overlayed on corresponding optical image of CtB labeled RGC boutons. E) Rotation of EM labeled RGC boutons to show clearest separation between island (green) and non-island boutons (magenta). Boutons innervating the one TC without a clear island or non-island identity are highlighted in white. F) Partial reconstructions of TCs indicated in panel B. TCs are color coded by whether the receive their input from the island or non-island RGC boutons. Positions of RGC inputs are indicated by white dots. G) Cumulative curve of the number of RGC boutons innervating reconstructed TCs. Circles indicate island / non-island identity. Numbers above the circles are the number of RGC boutons innervating the TC. Number within the circle indicates TC IDs used in other panels. H) Plot of the relationship between TC cell body (blue circle) and the location of the RGC inputs that innervate it (grey dots). Average synapse position is indicated by black circle. I) Results of Monte Carlo simulation predicting how many TCs would be innervated only by island or only by non-island RGC boutons if TC dendrites are independent of one another. Red bracket encloses 99% of results. J) EM reconstructions of the four example TCs highlighted in previous panels. Blue dots indicate the relative position of the four example cells. RGC boutons innervating the highlighted TC are in red. Island and non-island RGC boutons innervating other TCs shown in green and magenta.

LINs in the albino mouse dLGN. A) Reference image for panel B and C showing LINs (green) and CtB labeling of RGC terminals (magenta). B) Close association between targeted neurites of LINs (green) and RGC terminals (magenta). C) Four channels of confocal stack showing RGC bouton exclusion zone relative to LINs, cell nuclei, and reflective fibers. D) EM reconstruction of LIN relative to island (green) and non-island (magenta) RGC boutons. Input synapses from RGCs (blue circles) and output synapses (red triangles) are labeled. E) Two LIN dendrites labeled as in D. F) LIN dendrite labeled as in D. Inset shows magnified view of branch points.

Examination of the exclusion zone of four albino mice. Left and right dLGN is shown for each mouse. Contralateral projecting RGC terminals labeled with CtB (488 or 555) are shown in green. Mouse anti-synaptophysin immunoreactivity is shown in red. Anti-mouse secondary antibody labels blood vessels as well as anti-synaptophysin primary antibody. Reflected light highlighting myelinated axons is shown in blue. A) For each dLGN, as single plane of a confocal scan of the full dLGN (left) and exclusion zone (right) is shown. The white box indicates the position of the high-resolution image relative to the full dLGN. Bright punctate immune artifacts are visible in some panels. B) Closer look at exclusion zone of left dLGN from mouse 1 shown in panel A.

Renderings of partially reconstructed TCs. The top left key shows the position of the nuclei of the reconstructed TCs relative to the boundary of the RGC island (dotted white line). The color of the cell IDs indicates whether they received RGC input from the island or non-island RGC boutons. The star indicates a common reference position for all panels. RGC inputs innervating the TC highlighted in each panel are shown in red.

Proximal dendritic arbors of four example TCs where thin dendrites extending into the exclusion zone were observed. Neurites in the exclusion zone are indicated with stars. The location of RGC boutons innervating the TC are indicated by circles (green = island, magenta = non-island).