Correction: eIF2B conformation and assembly state regulate the integrated stress response
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Schoof M, Boone M, Wang L, Lawrence R, Frost A, Walter P. 2021. eIF2B conformation and assembly state regulate the integrated stress response. eLife 10:e65703. doi: 10.7554/eLife.65703.
Published 10 March 2021
In the published article, there is an error in the inline Table 2 for Oligos and sgRNAs. The Sequence for sgMS006 was incorrectly shown as “GTGTGTGGTTGTCATTAGGG” which is the sequence used to target eIF2α, not eIF2Bα. The correct sequence is “GAGGACGCCATGGACGACAA” and the correct target is eIF2Bα not eIF2β as erroneously stated in the table.
The corrected Table 2 is shown here:
Table 2. Oligos and sgRNAs.
| Oligo | Sequence | Use |
|---|---|---|
| MS266 | /5InvddT/G*G*G*A*A*CCTCTTCTGTAACTCCTTAGC | Amplify HDR template |
| MS267 | /5InvddT/C*C*T*G*A*G*GGCAAACAAGTGAGCAGG | Amplify HDR template |
| MS269 | TCGTGCCAGCCCCCTAATCT | Validate eIF2Bα tagging |
| MS270 | CTGAACGGCGCTGCTGTAGC | Validate eIF2Bα tagging |
| MS256 | AGTGAACTCTACCATCCTGA | Validate eIF2Bβ tagging |
| MS258 | TTAGGTGGACTCCTGTGC | Validate eIF2Bβ tagging |
| MS096 | CTGGCTAACTGGCAGAACC | Validate eIF2Bδ tagging |
| MS268 | AGAAACAAAGGCAGCAGAGT | Validate eIF2Bδ tagging |
| sgMS001 | CAATCTGCTTAGGACACGTG | Target Cas9 to eIF2Bβ C-terminus |
| sgMS004 | AGAGCAGTGACCAGTGACGG | Target Cas9 to eIF2Bδ C-terminus |
| sgMS006 | GAGGACGCCATGGACGACAA | Target Cas9 to eIF2Bα N-terminus |
| HDR: homology-directed recombination. | ||
The originally published Table 2 is shown for reference:
Table 2. Oligos and sgRNAs.
| Oligo | Sequence | Use |
|---|---|---|
| MS266 | /5InvddT/G*G*G*A*A*CCTCTTCTGTAACTCCTTAGC | Amplify HDR template |
| MS267 | /5InvddT/C*C*T*G*A*G*GGCAAACAAGTGAGCAGG | Amplify HDR template |
| MS269 | TCGTGCCAGCCCCCTAATCT | Validate eIF2Bα tagging |
| MS270 | CTGAACGGCGCTGCTGTAGC | Validate eIF2Bα tagging |
| MS256 | AGTGAACTCTACCATCCTGA | Validate eIF2Bβ tagging |
| MS258 | TTAGGTGGACTCCTGTGC | Validate eIF2Bβ tagging |
| MS096 | CTGGCTAACTGGCAGAACC | Validate eIF2Bδ tagging |
| MS268 | AGAAACAAAGGCAGCAGAGT | Validate eIF2Bδ tagging |
| sgMS001 | CAATCTGCTTAGGACACGTG | Target Cas9 to eIF2BβC-terminus |
| sgMS004 | AGAGCAGTGACCAGTGACGG | Target Cas9 to eIF2Bδ C-terminus |
| sgMS006 | GTGTGTGGTTGTCATTAGGG | Target Cas9 to eIF2β N-terminus |
| HDR: homology-directed recombination. | ||
The article has been corrected accordingly.
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© 2024, Schoof et al.
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Correction: eIF2B conformation and assembly state regulate the integrated stress response
eLife 13:e103865.
https://doi.org/10.7554/eLife.103865
