1. Immunology and Inflammation

Bronchus-associated macrophages efficiently capture and present soluble inhaled antigens and are capable of local Th2 cell activation

  1. Xin-Zi Tang
  2. Lieselotte S M Kreuk
  3. Cynthia Cho
  4. Ross Metzger
  5. Christopher D C Allen  Is a corresponding author
  1. University of California, San Francisco, United States
https://doi.org/10.7554/eLife.63296
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Cite this article
  1. Xin-Zi Tang
  2. Lieselotte S M Kreuk
  3. Cynthia Cho
  4. Ross Metzger
  5. Christopher D C Allen
(2022)
Bronchus-associated macrophages efficiently capture and present soluble inhaled antigens and are capable of local Th2 cell activation
eLife 11:e63296.
https://doi.org/10.7554/eLife.63296
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  3. Download .RIS

Abstract

In allergic asthma, allergen inhalation leads to local Th2 cell activation and peribronchial inflammation. However, the mechanisms for local antigen capture and presentation remain unclear. By two-photon microscopy of the mouse lung, we established that soluble antigens in the bronchial airway lumen were efficiently captured and presented by a population of CD11c+ interstitial macrophages with high CX3CR1-GFP and MHC class II expression. We refer to these cells as Bronchus-Associated Macrophages (BAMs) based on their localization underneath the bronchial epithelium. BAMs were enriched in collagen-rich regions near some airway branchpoints, where inhaled antigens are likely to deposit. BAMs engaged in extended interactions with effector Th2 cells and promoted Th2 cytokine production. BAMs were also often in contact with dendritic cells (DCs). After exposure to inflammatory stimuli, DCs migrated to draining lymph nodes, whereas BAMs remained lung resident. We propose that BAMs act as local antigen presenting cells in the lung and also transfer antigen to DCs.

Data availability

Relevant data are included in the manuscript figures and examples of 3D visualizations and time-lapse imaging are provided as videos. The RNAseq data have been deposited at the NCBI Gene Expression Omnibus (GEO) and are accessible through GEO Series accession number GSE214177 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE214177)

The following data sets were generated

Article and author information

Author details

  1. Xin-Zi Tang

    Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States
    Competing interests
    The authors declare that no competing interests exist.

  2. Lieselotte S M Kreuk

    Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States
    Competing interests
    The authors declare that no competing interests exist.

  3. Cynthia Cho

    Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States
    Competing interests
    The authors declare that no competing interests exist.

  4. Ross Metzger

    Department of Anatomy, University of California, San Francisco, San Francisco, United States
    Competing interests
    The authors declare that no competing interests exist.

  5. Christopher D C Allen

    Cardiovascular Research Institute, University of California, San Francisco, San Francisco, United States
    For correspondence
    Chris.Allen@ucsf.edu
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1879-9047

Funding

National Heart, Lung, and Blood Institute (DP2HL117752)

  • Xin-Zi Tang
  • Cynthia Cho
  • Christopher D C Allen

National Institute of Allergy and Infectious Diseases (R21AI130495)

  • Xin-Zi Tang
  • Cynthia Cho
  • Christopher D C Allen

UCSF Cardiovascular Research Institute

  • Xin-Zi Tang
  • Lieselotte S M Kreuk
  • Cynthia Cho
  • Christopher D C Allen

UCSF Sandler Asthma Basic Research Center

  • Xin-Zi Tang
  • Lieselotte S M Kreuk
  • Cynthia Cho
  • Christopher D C Allen

Agency for Science, Technology and Research

  • Xin-Zi Tang

National Institute of Allergy and Infectious Diseases (T32AI007334-31)

  • Lieselotte S M Kreuk

UCSF Program for Breakthrough Biomedical Research

  • Ross Metzger
  • Christopher D C Allen

National Heart, Lung, and Blood Institute (T32HL007731-28)

  • Lieselotte S M Kreuk

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Animal experimentation: The care, maintenance, and experimental manipulation of mice followedguidelines established by the Institutional Animal Care and Use Committee of the University of California, San Francisco under approved protocols AN079036, AN089524, AN111286, AN175836, and AN191685.

Copyright

© 2022, Tang et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Xin-Zi Tang
  2. Lieselotte S M Kreuk
  3. Cynthia Cho
  4. Ross Metzger
  5. Christopher D C Allen
(2022)
Bronchus-associated macrophages efficiently capture and present soluble inhaled antigens and are capable of local Th2 cell activation
eLife 11:e63296.
https://doi.org/10.7554/eLife.63296

Share this article

https://doi.org/10.7554/eLife.63296

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