A mechano-osmotic feedback couples cell volume to the rate of cell deformation
- Institut Curie, CNRS, UMR 144, France
- European Molecular Biology Laboratory, Germany
- Laboratoire Interdisciplinaire de Physique, France
- Sorbonne Université, INSERM, France
- Institut Curie, CNRS UMR168, France
Abstract
Mechanics has been a central focus of physical biology in the past decade. In comparison, how cells manage their size is less understood. Here we show that a parameter central to both the physics and the physiology of the cell, its volume, depends on a mechano-osmotic coupling. We found that cells change their volume depending on the rate at which they change shape, when they spontaneously spread are externally deformed. Cells undergo slow deformation at constant volume, while fast deformation leads to volume loss. We propose a mechano-sensitive pump and leak model to explain this phenomenon. Our model and experiments suggest that volume modulation depends on the state of the actin cortex and the coupling of ion fluxes to membrane tension. This mechano-osmotic coupling defines a membrane tension homeostasis module constantly at work in cells, causing volume fluctuations associated with fast cell shape changes, with potential consequences on cellular physiology.
Data availability
All data generated or analysed during this study are included in themanuscript and supporting file; all the raw analysed data shown in thefigure panels in the article are available in the accompanying SourceData files
Article and author information
Author details
Stéphane Vassilopoulos
Jean-François Joanny
Funding
Agence Nationale de la Recherche (ANR-19-CE13-0030)
- Matthieu Piel
Agence Nationale de la Recherche (ANR-10-EQPX-34)
- Matthieu Piel
Agence Nationale de la Recherche (ANR-10-IDEX-0001-02 PSL)
- Matthieu Piel
Agence Nationale de la Recherche (ANR-10-LABX-31)
- Matthieu Piel
Fondation pour la Recherche Médicale (FDT201805005592)
- Larisa Venkova
Human Frontier Science Program (LT000305/2018-L)
- Nishit Srivastava
Agence Nationale de la Recherche (ANR‐17‐CE13‐0020‐02)
- Amit Singh Vishen
European Union's Horizon 2020 research and innovation programme (Marie Sklodowska-Curie grant agreement no. 641639)
- Larisa Venkova
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2022, Venkova et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 6,941
- views
-
- 1,156
- downloads
-
- 57
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Download links
A two-part list of links to download the article, or parts of the article, in various formats.
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
A mechano-osmotic feedback couples cell volume to the rate of cell deformation
eLife 11:e72381.
https://doi.org/10.7554/eLife.72381
Further reading
-
- Cell Biology
Dynamic interactions between gut mucosal cells and the external environment are essential to maintain gut homeostasis. Enterochromaffin (EC) cells transduce both chemical and mechanical signals and produce 5-hydroxytryptamine to mediate disparate physiological responses. However, the molecular and cellular basis for functional diversity of ECs remains to be adequately defined. Here, we integrated single-cell transcriptomics with spatial image analysis to identify 14 EC clusters that are topographically organized along the gut. Subtypes predicted to be sensitive to the chemical environment and mechanical forces were identified that express distinct transcription factors and hormones. A Piezo2+ population in the distal colon was endowed with a distinctive neuronal signature. Using a combination of genetic, chemogenetic, and pharmacological approaches, we demonstrated Piezo2+ ECs are required for normal colon motility. Our study constructs a molecular map for ECs and offers a framework for deconvoluting EC cells with pleiotropic functions.
-
- Cell Biology
- Developmental Biology
A study in mice reveals key interactions between proteins involved in fibroblast growth factor signaling and how they contribute to distinct stages of eye lens development.
