1. Cell Biology
  2. Stem Cells and Regenerative Medicine

Full spectrum flow cytometry reveals mesenchymal heterogeneity in first trimester placentae and phenotypic convergence in culture, providing insight into the origins of placental mesenchymal stromal cells

  1. Anna Leabourn Boss  Is a corresponding author
  2. Tanvi Damani
  3. Tayla J Wickman
  4. Larry W Chamley
  5. Jo L James
  6. Anna ES Brooks
  1. University of Auckland, New Zealand
https://doi.org/10.7554/eLife.76622
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  1. Anna Leabourn Boss
  2. Tanvi Damani
  3. Tayla J Wickman
  4. Larry W Chamley
  5. Jo L James
  6. Anna ES Brooks
(2022)
Full spectrum flow cytometry reveals mesenchymal heterogeneity in first trimester placentae and phenotypic convergence in culture, providing insight into the origins of placental mesenchymal stromal cells
eLife 11:e76622.
https://doi.org/10.7554/eLife.76622
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Abstract

Single-cell technologies (RNA-sequencing, flow cytometry) are critical tools to reveal how cell heterogeneity impacts developmental pathways. The placenta is a fetal exchange organ, containing a heterogeneous mix of mesenchymal cells (fibroblasts, myofibroblasts, perivascular, and progenitor cells) . Placental mesenchymal stromal cells (pMSC) are also routinely isolated, for therapeutic and research purposes. However, our understanding of the diverse phenotypes of placental mesenchymal lineages, and their relationships remain unclear. We designed a 23-colour flow cytometry panel to assess mesenchymal heterogeneity in first-trimester human placentae. . Four distinct mesenchymal subsets were identified; CD73+CD90+ mesenchymal cells, CD146+CD271+ perivascular cells, podoplanin+CD36+ stromal cells, and CD26+CD90+ myofibroblasts. CD73+CD90+ and podoplanin+CD36+ cells expressed markers consistent with cultured pMSCs, and were explored further. Despite their distinct ex-vivo phenotype, in culture CD73+CD90+ cells and podoplanin+CD36+ cells underwent phenotypic convergence, losing CD271 or CD36 expression respectively, and homogenously exhibiting a basic MSC phenotype (CD73+CD90+CD31-CD144-CD45-). However, some markers (CD26, CD146) were not impacted, or differentially impacted by culture in different populations. Comparisons of cultured phenotypes to pMSCs further suggested cultured pMSCs originate from podoplanin+CD36+ cells. This highlights the importance of detailed cell phenotyping to optimise therapeutic capacity, and ensure use of relevant cells in functional assays.

Data availability

FCS data files have been provided for flow cytometry presented in Figures 2-3 following the link: http://flowrepository.org/public_experiment_representations/FR-FCM-Z4TJFigure 4:http://flowrepository.org/public_experiment_representations/FR-FCM-Z4TLFigure 7:http://flowrepository.org/public_experiment_representations/FR-FCM-Z5FV

Article and author information

Author details

  1. Anna Leabourn Boss

    Department of Obstetrics and Gynaecology, University of Auckland, Auckland, New Zealand
    For correspondence
    a.boss@auckland.ac.nz
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-1943-4162

  2. Tanvi Damani

    School of Biological Sciences, University of Auckland, Auckland, New Zealand
    Competing interests
    The authors declare that no competing interests exist.

  3. Tayla J Wickman

    Department of Obstetrics and Gynaecology, University of Auckland, Auckland, New Zealand
    Competing interests
    The authors declare that no competing interests exist.

  4. Larry W Chamley

    Department of Obstetrics and Gynaecology, University of Auckland, Auckland, New Zealand
    Competing interests
    The authors declare that no competing interests exist.

  5. Jo L James

    Department of Obstetrics and Gynaecology, University of Auckland, Auckland, New Zealand
    Competing interests
    The authors declare that no competing interests exist.

  6. Anna ES Brooks

    School of Biological Sciences, University of Auckland, Auckland, New Zealand
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3551-6982

Funding

Health Research Council of New Zealand (16/043)

  • Jo L James

University of Auckland (Doctoral Scholarship)

  • Anna Leabourn Boss

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Ethics

Human subjects: Placentae were collected following informed consent with approval from the Northern X Health and Disability Ethics Committee (NTX/12/06/057/AM09).

Copyright

© 2022, Boss et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Anna Leabourn Boss
  2. Tanvi Damani
  3. Tayla J Wickman
  4. Larry W Chamley
  5. Jo L James
  6. Anna ES Brooks
(2022)
Full spectrum flow cytometry reveals mesenchymal heterogeneity in first trimester placentae and phenotypic convergence in culture, providing insight into the origins of placental mesenchymal stromal cells
eLife 11:e76622.
https://doi.org/10.7554/eLife.76622

Share this article

https://doi.org/10.7554/eLife.76622

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