1. Developmental Biology
  2. Stem Cells and Regenerative Medicine

Identification of bipotent progenitors that give rise to myogenic and connective tissues in mouse

  1. Alexandre Grimaldi
  2. Glenda Comai
  3. Sebastien Mella
  4. Shahragim Tajbakhsh  Is a corresponding author
  1. Stem Cells and Development Unit, Institut Pasteur, France
  2. UMR CNRS 3738, Institut Pasteur, France
  3. Sorbonne Universités, Complexité du Vivant, F-75005, France
  4. Cytometry and Biomarkers UTechS, Institut Pasteur, France
  5. Bioinformatics and Biostatistics Hub, Institut Pasteur, France
https://doi.org/10.7554/eLife.70235
Share this article
Cite this article
  1. Alexandre Grimaldi
  2. Glenda Comai
  3. Sebastien Mella
  4. Shahragim Tajbakhsh
(2022)
Identification of bipotent progenitors that give rise to myogenic and connective tissues in mouse
eLife 11:e70235.
https://doi.org/10.7554/eLife.70235
  2. Download BibTeX
  3. Download .RIS

Abstract

How distinct cell fates are manifested by direct lineage ancestry from bipotent progenitors, or by specification of individual cell types is a key question for understanding the emergence of tissues. The interplay between skeletal muscle progenitors and associated connective tissue cells provides a model for examining how muscle functional units are established. Most craniofacial structures originate from the vertebrate-specific neural crest cells except in the dorsal portion of the head, where they arise from cranial mesoderm. Here, using multiple lineage-tracing strategies combined with single cell RNAseq and in situ analyses, we identify bipotent progenitors expressing Myf5 (an upstream regulator of myogenic fate) that give rise to both muscle and juxtaposed connective tissue. Following this bifurcation, muscle and connective tissue cells retain complementary signalling features and maintain spatial proximity. Disrupting myogenic identity shifts muscle progenitors to a connective tissue fate. The emergence of Myf5-derived connective tissue is associated with the activity of several transcription factors, including Foxp2. Interestingly, this unexpected bifurcation in cell fate was not observed in craniofacial regions that are colonised by neural crest cells. Therefore, we propose that an ancestral bi-fated program gives rise to muscle and connective tissue cells in skeletal muscles that are deprived of neural crest cells.

Editor's evaluation

This study combines sophisticated lineage tracing and single-cell RNAseq analysis to provide insights into cell fate decision in myogenesis and fibrogenesis. The paper will be of interest to a broad audience of developmental biologists, as it provides evidence for a population of novel bipotent cells, which possess a signature of both muscle and connective tissue.

https://doi.org/10.7554/eLife.70235.sa0

Introduction

Stromal cells that are associated with skeletal muscles play critical roles in providing structural support and molecular cues (Biferali et al., 2019; Kardon et al., 2003; Sefton and Kardon, 2019). The majority of muscle-associated connective tissues in the head is derived from cranial neural crest cells (NCCs), an embryonic cell population that contributes to most of the structural components of the ‘new head’, a vertebrate innovation (Le Douarin and Kalcheim, 1999; Gans and Northcutt, 1983; Grenier et al., 2009; Heude et al., 2018; Noden and Trainor, 2005). Recently, the extent of this contribution was redefined in muscles derived from cranial mesoderm, including extraocular (EOM), laryngeal and pharyngeal muscles (Comai et al., 2020; Grimaldi et al., 2015; Heude et al., 2018; Kuroda et al., 2021; Noden and Epstein, 2010). Interestingly, these muscles contain mesenchyme that is mesoderm-derived in their dorso-medial component, whereas the remaining muscle mass is embedded in mesenchyme that is neural crest-derived. It is unclear how the coordinated emergence of myogenic and connective tissues takes place during development, and how they establish long-lasting paracrine communication.

Along the trunk axis, paraxial somitic mesoderm gives rise to skeletal muscles and associated connective tissues (Burke and Nowicki, 2003). Upon signals emanating from adjacent tissues, the dermomyotome (dorsal portion of the somite) undergoes an epithelial-to-mesenchymal transition and gives rise to several cell types including all skeletal muscles of the body, vasculature, tendons and bones (Ben-Yair and Kalcheim, 2008; Christ et al., 2007). Similarly, cranial mesodermal progenitors give rise to these diverse cell types, yet, its unsegmented nature raises the question of how spatiotemporal control of these cellular identities is established. Moreover, cardiopharyngeal mesoderm, which constitutes the major portion of cranial mesoderm, has cardiovascular potential, which manifests in the embryo as regions of clonally related cardiac and craniofacial skeletal muscles (Diogo et al., 2015; Swedlund and Lescroart, 2020). This skeletal muscle/cardiac branchpoint has been the subject of intense investigation in several model organisms including ascidians, avians, and mouse (Wang et al., 2019). While cardiopharyngeal mesoderm was shown to give rise to connective tissues in the mammalian pharynx, the extent of its contribution to other craniofacial muscles in general has not been fully addressed (Adachi et al., 2020).

Recently, advanced pipelines integrating scRNAseq data and modern algorithms have been instrumental for identifying new lineage relationships during development (Cao et al., 2019; He et al., 2020; Qiu et al., 2021). Here, we employed lineage-restricted single-cell transcriptomics using multiple transgenic mouse lines combined with various computational methods, in situ labeling and loss-of-function experiments, and show that bipotent progenitors expressing the muscle determination gene Myf5 give rise to both skeletal muscle and anatomically associated connective tissues. Surprisingly, this property was restricted to muscle masses lacking NCC-derived connective tissues, indicating that cranial mesoderm acts as a source of connective tissues in the absence of neural crest cells.

Results

Myogenic and non-myogenic mesodermal populations coexist within distinct head lineages

Somitic (Pax3-dependent) and cranial (Tbx1/Pitx2-dependent) mesoderm give rise to diverse cell types including those of the musculoskeletal system (Figure 1A). We first set out to explore the emergence of skeletal muscles and other associated mesodermal tissue within these programs. To that end, we employed a broad anterior mesoderm lineage-tracing strategy using the Mesp1Cre/+;Rosa26mTmG/+ line as it labels cranial-derived mesoderm and the anterior somites (Heude et al., 2018). At E10.5, when craniofacial skeletal muscles start to be specified, the upper third (anterior to forelimb) of the embryos was dissected, live GFP+ cells were isolated by FACS, and processed for scRNAseq analysis (Figure 1—figure supplement 1A-C). After removal of doublets and lower quality cells (see Materials and methods), a large portion of the cells obtained by Mesp1Cre/+;Rosa26mTmG/+ lineage tracing segregated as individual clusters expressing markers of adipogenic, chondrogenic, sclerotomal, endothelial, and cardiovascular lineages as well as the foregut and primitive lung mesenchyme (Figure 1B, Figure 1—figure supplement 2A-B). Pax3, Pitx2, Tbx1, Myf5, and Myod expression were used to identify clusters containing the cranial myogenic progenitors, annotated as ‘Cardiopharyngeal mesoderm’ and ‘Anterior somite’ (Figure 1B–C, Figure 1—figure supplement 2A).

Figure 1 with 3 supplements see all
Download asset Open asset
scRNAseq reveals non-myogenic populations of cranial mesoderm lineages.

(A) Scheme of connective tissue origin in the head and known mesodermal upstream regulators. E: Eye, 1–4: Pharyngeal arches 1–4. (B–F) scRNAseq analysis on Mesp1Cre/+; Rosa26mTmG/+ embryos at E10.5 (2 datasets of 2 embryos were aggregated to generate this data, see methods). (B) UMAP of Mesp1Cre/+; Rosa26mTmG/+ E10.5 scRNAseq with main cell types highlighted. The clusters ‘Anterior somite’ and ‘Cardiopharyngeal mesoderm’ were subsetted for further analysis below. (C) UMAP expression plots of Pitx2 (EOM), Tbx1 (cranial mesoderm except EOM) and Pax3 (somitic mesoderm), indicating the clusters of progenitors that were selected. (D) UMAP of progenitor subset annotated as myogenic and non-myogenic based on expression patterns found in E and F. (E) UMAP expression plots of Pitx2, Tbx1 and Pax3 in the Mesp1Cre/+; Rosa26mTmG/+ E10.5 subset. (F) Heatmap of top 20 markers of myogenic versus non-myogenic clusters Mesp1Cre/+; Rosa26mTmG/+ E10.5 subset. Pdgfra/Pdgfa genes are highlighted.

After subsetting these clusters (‘Cardiopharyngeal mesoderm’ and ‘Anterior somite’), a few subclusters clearly separated based on their origin and anatomical location (Figure 1D–E, Figure 1—figure supplement 2C). Surprisingly, about half of the supposedly myogenic cells exhibited a connective tissue signature, including a strong bias toward Prrx1, a marker of lateral plate mesoderm (Durland et al., 2008), Col1a1, a major extracellular matrix component of connective tissue cells (De Micheli et al., 2020), and Twist1, a key determinant for the mesenchymal properties of cranial mesoderm (Bildsoe et al., 2016; Figure 1F). Furthermore, the expression of Pdgfra, a well-defined marker of stromal cells (Farahani and Xaymardan, 2015), was robustly anticorrelated with the expression of its ligand Pdgfa and associated with non-myogenic genes. Conversely, Pdgfa, was correlated with a myogenic cell state (Figure 1F, Figure 1—figure supplement 3A-B). Of note, myogenic Pdgfa expression was shown to promote adjacent sclerotomal cells to adopt a rib cartilage fate (Tallquist et al., 2000). Therefore, this analysis identified anatomically distinct muscle and closely associated connective tissue progenitors and highlights a potential PDGFR-mediated crosstalk between these 2 cells types.

Transcriptional trajectories reveal a myogenic to non-myogenic cell state transition

To understand the lineage relationship between myogenic and non-myogenic cells, we exploited the unspliced and spliced variants of our scRNAseq data, and computed the RNA velocity in each cell, using a recently described tool (Bergen et al., 2020; Figure 2, Figure 2—figure supplement 1). RNA velocity interrogates the relative abundance of unspliced and spliced gene variants, which depends on the rates of transcription, degradation, and splicing to infer directional trajectories (Bergen et al., 2020; La Manno et al., 2018). The cell cycle status constitutes a potential bias in scRNAseq data, especially when heterogeneous populations undergo cellular expansion, commitment and differentiation (McDavid et al., 2016). To eliminate this potential bias, cell cycle genes were consistently regressed out during preprocessing and directional trajectories were overlaid with cell cycle phase visualization for comparisons (Figure 2—figure supplement 1A, Materials and methods). Notably, RNA velocity-inferred trajectories suggested that Myf5+ cells from the myogenic compartment contributed to non-myogenic cells (Figure 2A). These calculations were based on gene- and cluster-specific dynamics, which yield higher accuracy than the initially described RNA velocity method, while providing quantitative metrics for quality control (Figure 2—figure supplement 1B-D and Materials and methods).

Figure 2 with 1 supplement see all
Download asset Open asset
Transcriptomic dynamics reveal a myogenic to non-myogenic transition in anterior somite progenitors.

(A) Velocity UMAP plots of Mesp1Cre/+; Rosa26mTmG/+ embryos at E10.5 displaying myogenic and non-myogenic clusters. Arrows represent the lineage progression based on RNA velocity (relative abundance of unspliced and spliced transcripts). (B) Heatmap of driver genes accounting for anterior somite velocity, highlighting Pdgfra. Driver genes are genes that are transcriptomically active in a given cluster. (C) Phase portraits of few selected driver genes in the anterior somites: Foxp1, Meox2, Meis1, Twist2, Fap, Pdgfra, Prrx1, and Pcolce. Y-axis represents the amount of unspliced transcript per cell; X-axis represents the number of spliced transcripts per cell. A high fraction of unspliced variants indicates an active transcription of the locus, while the inverse indicates inactive/repressed transcription. Dynamics of transcription were inferred at a gene- and cluster-specific level (see Methods). (D) Phase portraits, RNA velocity and expression plots of Pdgfa and Pdgfra showing splicing dynamics of these two genes. (E) Working model of myogenic and non-myogenic fate decisions from a common bipotent progenitor in anterior somites.

Another powerful feature of this method is the ability to infer ‘driver genes’ that are responsible for most of the calculated RNA velocity, hence actively transcribed, or repressed (Bergen et al., 2020). Therefore, these genes can identify transitory states underlying cell fate decisions. We used this approach to uncover the driver genes that were responsible for the velocity found in anterior somites, as these cells displayed the most consistent directionality, and appeared to be independent of cell cycle (Figure 2B, Figure 2—figure supplement 1A, Table 1). Top transcribed driver genes included Foxp1 (Shao and Wei, 2018), Meox2 (Noizet et al., 2016), Meis1 (López-Delgado et al., 2020), Twist2 (Franco et al., 2009), Fap (Puré and Blomberg, 2018), Pdgfra (Tallquist et al., 2000), Prrx1 (Leavitt et al., 2020), and Pcolce (Bildsoe et al., 2016; Figure 2C), which are associated with fibrosis and connective tissue development. Interestingly, we noted that Pdgfra appeared as a driver gene and was activated along this inferred trajectory, whereas Pdgfa expression decreased rapidly (Figure 2D). Taken together, RNA velocity analysis for anterior somite mesodermal progenitors showed that Myf5+/Pdgfa+ cells shifted toward a non-myogenic fate, which includes the downregulation of Myf5 and Pdgfa and the activation of Pdgfra expression (Figure 2E).

Table 1
Driver genes underlying cell fate decisions in each dataset.
E10.5 Anterior somitesE11.5 EOM MyogenicE11.5 EOM Non-myogenicE12.5 Non-myogenicE14.5 Non-myogenic
Tshz2Ccdc141Zfpm2Mgat4cDnm1
Eya1Mcm6Plxna4CenpvPid1
C1qtnf3Dync1i1Col23a1C130073E24RikNrp2
Meis2Tpm2Edil3Tbx3os1Ntrk3
Limch1Celf2Map2E330013P04RikTmem132c
Moxd1Sox6RoraStk26Egflam
Epha4TncSema5aEdil3Gpr153
Pitx2Magi3Colec12Fdft1Efemp1
Parm1Sh3glb1Smoc1Lima1Adamts2
Hpse2Parm1PtprtTrim59Brinp1
Lrrn1Ephb1Ror1Meg3Vegfc
Dmrt2Bmpr1bDock5Gins3Twist2
Myl3HellsMap1bTpm2Itgb5
FapPdgfcFn1Cdh6Gria1
Hs6st2PtprdLimch1Csmd3Sned1
Ddr2Cnr1Tenm4Tceal5Sorcs3
Cald1Sema3dRbms3PclafEbf2
Prrx1Clcn5Srgap3Tspan9Fam19a1
Magi3Chd7Tmem132cEps8Trabd2b
Ntn1Col25a1Sdc2LmnaPlxdc2
Zfhx3Reep1Add3Dmrt2Sh3gl3
Meis1Ctnnal1PdgfraCpeb4Luzp2
Tnni1Tpm1GmdsHpgdPdzd2
CrymZim1St6galnac3Rcsd1Sema3e
Ebf1Lmx1aEpb41l3PdgfraRims1
Nr2f1NebPde3aPlac1Epha3
Ntng1Atad2ToxPalmdCyp7b1
Pgm5Dapk2Smarca2Gucy1a1Gem
Cdh6Prox1CtdsplWif1Ldb2
Foxp1LsampMagi2Naalad2Scube1
Celf2TtnDpysl3Smoc2Pdgfra
Tbx1Pls3Fgfr2Rassf4Pde1a
BdnfSlf2Ldb2Pttg1Nde1
Colec12Vat1lIgf1Josd2Enpp2
Eya4E2f1Elk3Plxna4Fam107b
SobpEpb41l2Zmiz1Eya2Stxbp6
Peg3Gm28653Dlc1Nrsn1Rerg
PdgfraLrrn1NhsFignPrex2
NrkMef2cCdkn1cInppl1Man1a
PtnSt8sia2Plpp3Rnf152Tmem45a
Daam1Tshz1Ebf1Lasp1Sh3bp4
Dlk1Wee1Sorbs2MrlnMcc
Unc5cSlc24a3Baz1aCdt1Ncald
Lpar1Ncoa1Fat4Notch3Kdelr2
Syne2DekGolgb1Pax3Pcdh19
Nkd2Kdm5bHpse2EgfrGas7
Brinp1Unc13cSamd4Dbf4Cpt1c
Zfhx4Ddr1Itga9BcrAdam22
NnatPip4k2aMagi1Mllt3Itgb8
Gxylt2Fndc3c1Pcdh9Nectin1Dchs2
ClmpRbm24Tgfbr2Grin3aCep350
Ror2Rreb1Ntf3Cbfa2t3Oat
NfiaRragdCol11a1Cdh2Rab30
Ebf2Acsl3Runx1t1AnlnAff2
EdnraAcvr2aTnrc18Ccdc6Gna14
Fli1Zeb1CrymMcuSlc29a1
Tspan12RgmaFapFnip2Pls3
Ttc28Arpp21Ppp1r1aKcnk13Traf3ip1
NfibLef1TesSned1Rcsd1
Ccdc88cNr2f2Bicc1Nde1Lgr4
Col13a1Foxo1Il1rapl1Hipk3Zfp9
2700069I18RikPdzrn4AlcamArhgap11aHs3st5
PcolceHmga22700069I18RikFam8a1Aspn
Scn3aLurap1lDab2Kif21aNrxn1
Acvr2aPkigCntlnMtss1Rrm1
Auts2NclClmnAbcd2Igfbp7
Col3a1CT025619.1Rbms1Irx5Slc35f3
Gap43Erbb4Tmem2Pacs2Kif15
MrlnCdk14Cdh6Nab1Slc1a3
Pax3Kif21aLypd6Ccnd2Bmp6
Sim1Zfp704Mmp2BokDkk2
Epb41l2NaspKif5cDok5Tspan9
Ppp3caPlekha5Cadm2NcapgEts1
Tnfaip6Cap2Prkg2Rfx8Gria3
Tmem132cSncaCped1Fhod3Sox8
Tmem2Epha4DtlTk1Melk
Epb41l3Atad5Ror2Asf1bNtm
Crybg3Cntn3UtrnTekSynpo2l
Nrxn1Cacna2d1Foxp1Arfgef3Hlf
Farp1Pak3L3mbtl3Rnf182Adamts5
Sulf1Megf10Cdh23Kif14Plcb4
Tmtc2Tnnt1Negr11810041L15RikCdc25b
Pde4dipActa2Hmcn1Rrm2Mgat4a
Phldb2Barx2Col26a1Fgf5Mdfic
Plpp3MrlnFbn2Barx2Trpc5
Ybx3Pgm5Ankrd12Fli1Kif4
Ppm1lFmr1LhfpJph2Plce1
Twist2Smc4Hs3st3b1Dtx4Il17rd
Nuak1ClmpAdgrl3NcaldMmp16
Tgfb2Alpk2SvilZic4Hhip
Sfrp1Kctd1Mob3bDlc1Tpx2
SncaipMeg3Trabd2bCdc45Ndc80
Tenm3Samd5RmstGatmBub1b
Cdh2NrkPrrx1Ssc5dHmmr
Iqgap2Piezo25330434G04RikPhactr2Kank4
AppRobo1Zfhx3Ppp1r14cTmeff2
Pgam2Col1a2Foxp2AglNr4a1
Rspo3CntrlMpp6Tox3Aurkb
CdonMllt3Crispld1AurkaLrrtm3
Ebf3Peg3Eya1Cdh15Cenpq

Myf5-derived lineage contributes to connective tissue cells in the absence of neural crest

Given that the number of cells examined in the EOM and pharyngeal arch mesodermal clusters from the E10.5 dataset was lower than for anterior somites, we decided to validate the relevance of Myf5-derived non-myogenic cells in these cranial regions directly in vivo. We thus examined the EOM, larynx and upper back muscles in the early fetus at E14.5 using a Myf5-lineage reporter mouse (Myf5Cre/+; Rosa26TdTomato/+) combined with a contemporary reporter for Pdgfra (PdgfraH2BGFP/+) (Figure 3). Notably, we observed GFP+ TOM+ double-positive cells in regions of EOM, laryngeal and upper back muscles that are partially or fully deprived of neural crest (Adachi et al., 2020; Comai et al., 2020; Heude et al., 2018; Figure 3A–C'). Conversely, no double-positive cells were detected in muscles that are fully embedded in neural crest derived mesenchyme such as mandibular and tongue muscles (Heude et al., 2018; Figure 3D–E').

Figure 3
Download asset Open asset
Myf5-derived mesodermal connective tissue partially compensates for the lack of neural crest.

(A-E') Transverse sections of an E14.5 Myf5Cre/+; Rosa26TdTomato/+; PdgfraH2BGFP/+ embryo immunostained for Myod/Myog. White arrowheads indicate cells double-positive GFP/TOM and negative for Myod/Myog (n = 3 embryos). (F-I') Transverse cryosections of the EOM at E13.5 of Wnt1Cre/+; Rosa26mTmG/+; Myf5nlacZ/+ (G,I) and Mesp1Cre/+; Rosa26mTmG/+; Myf5nlacZ/+ (F,H) immunostained for β-gal, at the level of the medial attachment (F,G) and lateral muscle masses (H,I). Yellow arrowheads indicate Myf5-expressing cells in the context of mesodermal and neural crest lineages. Note that Myf5-expressing cells are mGFP+ in the Mesp1 lineage and mGFP- in the Wnt1 lineage. Red arrowheads indicate neural-crest cells that are excluded from the Myf5 lineage (n = 2 embryos for each). (J) Scatter plots of the proportion of double positive cells in E14.5 Myf5Cre/+; Rosa26TdTomato/+; PdgfraH2BGFP/+ embryos in various regions throughout the EOM (the line is the mean, each dot is a tissue section, each color is a different embryo, n = 3 embryos). (K) Scheme highlighting the quantified regions in (J) and summarising the contribution of each population to periocular connective tissues. TOM: TdTOMATO.

Figure 3—source data 1

Excel table summarizing the quantification displayed on Figure 3J.

https://cdn.elifesciences.org/articles/70235/elife-70235-fig3-data1-v3.xlsx

Mesenchymal tissues associated with the EOM arise from mesoderm in its most dorso-medial portion and from neural crest in its ventro-lateral portion (Comai et al., 2020; Kuroda et al., 2021). This dual origin makes it a prime candidate to explore the relative contribution of Myf5-derived cells to the associated connective tissues within a single functional unit. Using Wnt1Cre/+; Rosa26mTmG/+; Myf5nlacZ/+ (NCC tracing with Wnt1) and Mesp1Cre/+;Rosa26mTmG/+;Myf5nlacZ/+ (mesoderm tracing with Mesp1) at E13.5, we found that GFP+ cells that expressed Myf5 (β-gal+) were exclusively present in Mesp1-derived domains and absent from the Wnt1 lineage (Figure 3F–I'). To further evaluate the contribution of Myf5-derived cells to connective tissues in either domain, we re-examined the Myf5Cre/+; Rosa26TdTomato/+; PdgfraH2BGFP/+mouse line and quantified the percentage of GFP+ TOM+ cells in the EOM. As expected, we observed a medio-lateral gradient of Myf5-lineage contribution to EOM-associated connective tissues by E14.5, and this was anticorrelated with the local contribution of neural crest cells to connective tissues (Figure 3J–K). Thus, in agreement with our scRNAseq velocity analysis, these observations suggest that the mesodermal Myf5-lineage contributes to muscle-associated connective tissue in domains that are deprived of neural crest mesenchyme.

Myf5-derived cells can maintain a molecular crosstalk following bifurcation into myogenic and connective tissue fates

To identify the transition between these two fates, we generated an additional sc-RNAseq dataset based on Myf5-lineage tracing at E11.5 (Myf5Cre/+;Rosa26mTmG/+) and produced RNA velocity streams (Figure 4A, Figure 4—figure supplement 1). We focused on the EOM and anterior somites, which were clearly distinguished as independent clusters based on the expression of Alx4 (Bothe and Dietrich, 2006) and Pax3 (Heude et al., 2018), respectively (Figure 4B). In agreement with the E10.5 mesodermal (Mesp1) sc-RNAseq dataset, these progenitors presented a strong dichotomy in Pdgfa and Pdfgra expression between myogenic and non-myogenic cells, respectively (Figure 4—figure supplement 1D). Moreover, RNA velocity suggested more myogenic to non-myogenic conversion (Figure 4A, Figure 4—figure supplement 1E-H). To explore further the cell fate transition in these regions, we used a recently described approach by creating a ‘Coexpression score’ based on myogenic and non-myogenic signatures (Kameneva et al., 2021) (see Materials and methods, Figure 4C). This analysis revealed that individual cells undergo a progressive switch from myogenic to non-myogenic gene expression along the inferred trajectories, where cells at the transition zone shut off the myogenic program and start activating fibrogenic genes (Figure 4C heatmap).

Figure 4 with 2 supplements see all
Download asset Open asset
Maintenance of signaling cues between Myf5-derived myogenic and non-myogenic cells in EOM.

(A–D) scRNAseq analysis of the Myf5Cre/+; Rosa26mTmG/+ E11.5 dataset (2 datasets of 2 embryos were aggregated to generate this data, see Materials and methods). (A) UMAPs of Myf5Cre/+; Rosa26mTmG/+ E11.5 RNA velocity trajectories. (B) Expression plots of Alx4 and Pax3, highlighting EOM and Anterior somite clusters, respectively. (C) Plots of Myogenic and Non-myogenic signatures, Coexpression score and heatmaps of top markers, highlighting the transition population in EOM and anterior somites. Cells are ordered based on their non-myogenic signature score (increasing). The coexpression score is the product of the myogenic and non-myogenic signatures. Cells presenting a coexpression score higher than 0.20 are highlighted in yellow. These cells represent the transition between the myogenic and non-myogenic fates. (D) UMAP of the EOM subset revealing the bipartite fate of Myf5-expressing cells. (E-G’) RNAscope on Myf5Cre/+; Rosa26mTmG/+ E14.5 tissue sections with Pdgfra (cyan) and Pdgfa (red) probes (E-E’’), Bmprb1 (red) and Bmp4 (cyan) probes (F-F’) and Ephb1 (red) and Efnb1 (cyan) probes (G-G’). Myf5-derived cells are labelled by membrane GFP staining (n = 3 embryos each). Red and yellow arrowheads indicate Myf5-derived myogenic and non-myogenic cells respectively. The dotted lines highlight the boundary of the muscle masses. (H) Quantification of the Ligand-Receptor scores for each pair (see Materials and methods). Note that these ratios are negative in the case of Bmp and Eph (signaling from non-myogenic to myogenic) but positive for Pdgf (signaling from myogenic to non-myogenic). (G) Model of myogenic and non-myogenic cell communication following bifurcation from a bipotent cell.

Figure 4—source data 1

Excel table summarizing the quantification displayed on Figure 4H.

https://cdn.elifesciences.org/articles/70235/elife-70235-fig4-data1-v3.xlsx

To investigate in more detail potential paracrine cell-cell communication between myogenic and non-myogenic cells, we examined their expression patterns within the EOM, given its well-defined morphology (Comai et al., 2020), and its strong myogenic/non-myogenic bi-directional cell-fate (Figure 4D). We performed single molecule fluorescent in situ hybridization (RNAscope) for Pdgfa and Pdgfra on E14.5 lineage-traced Myf5Cre/+;Rosa26mTmG/+ fetuses (Figure 4E). In accordance with the scRNAseq analysis, we observed cells exhibiting a mostly non-overlapping, complementary pattern of Pdgfa and Pdgfra transcripts within the Myf5-derived lineage, while retaining anatomical proximity, even at later stages of EOM development.

Gene set enrichment analysis of EOM myogenic and non-myogenic driver genes showed that transmembrane receptor protein kinase and SMAD activity were shared terms between the two clusters, suggesting that specific complementary signaling networks could be actively maintained between these two populations (Figure 4—figure supplement 2A). Both signaling pathways were reported to act as inhibitors of myogenic differentiation and could therefore be associated with progenitor cell maintenance (Arnold et al., 2020; Cossu et al., 2000). Notably, Bmpr1b and Ephb1 were among the top 100 driver genes of the myogenic EOM compartment, suggesting that myogenic commitment is associated with upregulation of these kinase receptors in the EOM (Figure 4—figure supplement 2B, Table 1). Strikingly, two of their respective ligands, Bmp4 and Efnb1, were preferentially expressed in non-myogenic cells. Analysis of their expression patterns in E14.5 embryos by RNAscope validated these complementary expression patterns in adjacent muscle and connective tissue domains (Figure 4F–H). These results favor a model where paracrine signaling networks operate between myogenic and non-myogenic Myf5-derived cells (Figure 4I), while their cellular juxtaposition is maintained through fetal stages.

Obstructing myogenesis expands connective tissue formation from bipotent cells

The directional trajectories identified by RNA velocity in the EOM at E11.5 showed a strong bidirectionality in fate with a higher velocity confidence index at each end of the myogenic and non-myogenic domains, and lower at their interface (Figure 5—figure supplement 1A). This suggested that cells at the interface are bipotential while cells located on either side of this central region are committed either to a myogenic-or non-myogenic fate. To identify the regulatory factors underlying this potential bipotency, we used SCENIC, a regulatory network inference algorithm (Aibar et al., 2017). This tool allows regrouping of sets of correlated genes into regulons (each regulon consists of a transcription factor and its targets) based on binding motifs and co-expression. The top regulons of this analysis revealed active transcription factors underlying myogenic and non-myogenic cell fates in the EOM at E11.5. Notably, Myf5, Pitx1, Mef2a, and Six1, transcription factors known to be implicated in myogenic development (Buckingham and Rigby, 2014), appeared among the top regulons in myogenic cells whereas Fli1, Ebf1, Ets1, Foxc1, Meis1, and Six2, genes known for their involvement in adipogenic, vascular, mesenchymal and tendon development (Jimenez et al., 2007; López-Delgado et al., 2020; Noizet et al., 2016; Truong and Ben-David, 2000; Whitesell et al., 2019; Yamamoto-Shiraishi and Kuroiwa, 2013), constituted some of the highly active non-myogenic transcription factors (Figure 5A). Interestingly, recent work uncovered Fli1 as a potential regulator of vascular fate in multipotent myogenic progenitors (Ferdous et al., 2021). Accordingly, we found that Scube1, a gene known for its involvement in vasculature development, was upregulated in the Pdgfra+ non-myogenic fraction of the EOM (Figure 5—figure supplement 1B). RNAscope in situ hybridization confirmed these findings and showed that Scube1 was expressed at the level of the EOM medial attachment at E14.5 (Figure 5—figure supplement 1C-E). In addition, Scube1 was reported to promote BMP signaling (Liao et al., 2016). Thus, the EOM tendon attachment seems to rely on transcription factors and markers that are typically vascular, hence suggesting that some of them are coopted.

Figure 5 with 1 supplement see all
Download asset Open asset
Disruption of Myf5 increases the connective tissue output from bipotent cells.

(A) Heatmap of top regulons (transcription factor and associated targets) of the EOM subset of the Myf5Cre/+; Rosa26mTmG/+ E11.5 dataset. The suffix ‘_extended’ indicates that the regulon includes motifs that have been linked to the TF by lower confidence annotations, for instance, inferred by motif similarity. Number in brackets indicates number of genes comprising the regulon (n = 2 pooled datasets). (B–C) Transverse sections of Myf5nlacZ/+ (B), and Myf5nlacZ/nlacZ (C) embryos in the EOM region at E12.5 immunostained for β-gal (green), and Myod/Myog/Pax7 (red). Red arrowheads indicate β-gal/ Myod/Myog/Pax7 double positive cells in control EOM/Masseter and in mutant Masseter. Asterisk highlights the lack of myogenic progenitors in the EOM region of the mutant embryo, indicated by the absence of Myod/Myog/Pax7 staining. (D-E’) Transverse sections of Myf5nlacZ/+ (D-D'), and Myf5nlacZ/nlacZ (E-E') in the EOM region at E12.5 immunostained for β-gal (green), Sox9 (red), and Myod/Myog/Pax7 (gray). Yellow arrowheads indicate β-gal/Sox9 double positive cells and show an expansion of this cell population in the mutant. (F) Quantification of proportion of β-gal+;Sox9+ double positive cells in the total Sox9+ population of the EOM and Masseter muscles. Each dot is a different sample, the center line of the boxplot is the median value. (n = 3 embryos, p-values were calculated using a two-sided Mann-Whitney U test). (G-I’) Transverse sections of MyodiCre/+; Rosa26TdTomato/+; PdgfraH2BGFP/+ embryos at E14.5 immunostained for Myod/Myog (committed and differentiating myoblasts) in the extraocular (G-G’’), mandibular (H-H’), and back muscles (I-I’). White arrowhead indicates double positive cells (GFP+ TOM+). (J) Quantification of double positive cells (GFP+ TOM+) in EOM, mandibular muscles and back muscles per 100 μm2 area on MyodiCre/+; Rosa26TdTomato/+; PdgfraH2BGFP/+ sections shown in E-G (n = 4 embryos). (K) Model of lineage progression from bipotent cells in a Myf5 null background.

Figure 5—source data 1

Excel table summarizing the quantification displayed on Figure 5F.

https://cdn.elifesciences.org/articles/70235/elife-70235-fig5-data1-v3.xlsx
Figure 5—source data 2

Excel table summarizing the quantification displayed on Figure 5J.

https://cdn.elifesciences.org/articles/70235/elife-70235-fig5-data2-v3.xlsx

Given that Myf5 appeared itself as a top myogenic regulon (Figure 5A), we interrogated the fate of Myf5-expressing progenitors in a Myf5nlacZ/nlacZ null embryos at E12.5 (Figure 5B–E’). As previously reported, the EOMs are absent in this mutant (Figure 5C, asterisk) (Sambasivan et al., 2009). Interestingly, some β-gal+ cells were found in the cartilage primordium (Sox9+) of the EOM in the heterozygous control indicating that cells with recent Myf5 activity diverged to this non-myogenic fate (Figure 5D–D'). Notably, disruption of Myf5 activity led to a threefold increase in the proportion of non-myogenic Myf5-derived cells in this region (Figure 5E–F). In contrast, no double-positive cells were found in the masseter, a muscle fully embedded in neural crest-derived connective tissue, even in the absence of Myf5 (Figure 5F). Myf5 expression is thus necessary to maintain a balance between myogenic and non-myogenic cell fates of Myf5+ progenitors only in neural crest-depleted regions. In contrast, very few Pdgfra+ cells were found to be derived from Myod expressing cells in MyodiCre;Rosa26TdTomato/+;PdgfraH2BGFP/+ fetuses at E14.5, particularly in the EOM and the back muscles (about 3 and 1.5 cells per 100 μm2 of muscle, respectively)(Figure 5G, I, J). Accordingly, the masseter lacked Myod-derived connective tissue cells (Figure 5H and J). These observations indicate that progenitors that bifurcate to myogenic and non-myogenic cell fates are present only in neural-crest depleted regions. This property is associated primarily with Myf5 expression, as subsequent activation of Myod within this lineage appears to lock cell fate into the myogenic program and suppress their connective tissue potential (Figure 5K).

Myf5-derived connective tissues are observed in fetal stages

Although we identified Myf5-derived non-myogenic cells in various regions of the embryo, it was not clear if this population was continuously generated throughout development. To address this issue, we performed two more scRNAseq experiments at E12.5 and E14.5, using contemporary Myf5 labeling, which led to much fewer non-myogenic cells that could be captured (Myf5GFP-P/+; Figure 6, Figure 6—figure supplement 1, Figure 6—figure supplement 2). In accordance with the earlier datasets, some cells that appeared to belong to muscle anlagen of EOM, somites and caudal arches progressed toward a non-myogenic state (Figure 6A–C’). To assess the identity of these cells, we performed a gene set enrichment network analysis combining the differentially expressed genes of non-myogenic clusters of all stages. We found that all stages contributed relatively equally to each ‘GO Molecular Function’ and ‘Reactome pathways’ terms despite their relatively diverse gene expression signatures (Figure 6D–E’, Figure 6—figure supplement 3). This suggests that these non-myogenic cells are relatively homogeneous in gene signatures throughout cranial muscles. Highly significant terms hinted at a myogenic-supporting role, providing muscle progenitors with extracellular matrix components, and contributing to neuronal guidance (Figure 6E). Among these terms, presence of Pdgf signalling and receptor kinase activity indicated, once again, that the interactions found in the EOM could occur also at later stages in various craniofacial muscles that are deprived of neural crest derived connective tissue.

Figure 6 with 3 supplements see all
Download asset Open asset
Myf5-derived non-myogenic cells are generated continuously up to fetal stages.

(A-C') RNA velocity plots of Myf5Cre/+; Rosa26mTmG/+ E11.5, Myf5GFP-P/+ E12.5 and Myf5GFP-P/+ E14.5 datasets (n = 2 pooled datasets, n = 1 embryo and n = 1 embryo, respectively) displaying cell-type annotation (A–C) and myogenic and non-myogenic clustering (A’-C’). The dotted boxes highlight the transitions to non-myogenic clusters in each dataset. (D–E) Gene ontology network of GO Molecular Function and Reactome pathway performed on combined top 100 markers using Cluego. These terms were generated using the sum of all differentially expressed genes of the non-myogenic clusters across all datasets (see Materials and methods). (D’-E’) Relative contribution of each stage to term node represented as piecharts (i.e. the proportion of genes underlying this term coming from that stage). Dotted boxes highlight the shared tyrosine kinase and PDGF signaling pathways.

A novel regulatory network underlies the non-myogenic cell fate

Myf5+ bipotent progenitors were observed at multiple stages and anatomical locations, and they yielded a relatively homogeneous population expressing common markers associated with extracellular matrix components, cell adhesion molecules, and kinase signalling. To assess whether the regulatory mechanisms guiding this transition are distinct in different locations in the head, we set out to explore the common molecular switches underlying cell fate decisions. To do so, we developed a pipeline where we combined the list of driver genes at the start of the non-myogenic trajectory (Table 1) with the most active regulons in the non-myogenic region (Materials and methods, code in open access). This resulted in a network consisting of the most active transcription factors and the most transcriptionally dynamic genes found at the non-myogenic branchpoint. We performed this operation for each dataset independently and displayed them as individual networks (Figure 7—figure supplement 1A-D). Finally, we overlapped the list of these ‘driver regulators’ to identify the common transcription factors guiding the non-myogenic cell fate decision (Figure 7A). Notably, Foxp2, Hmga2, Meis1, Meox2, and Tcf7l2 were identified in all four scRNAseq datasets as key driver regulators, and thus are likely to play significant role in the non-myogenic transition (Figure 7A, Table 2).

Figure 7 with 2 supplements see all
Download asset Open asset
A shared program involving Forkhead-box transcription factors supports non-myogenic fate transition at various stages and anatomical locations.

(A) Barplot displaying frequency of appearance of most predominant transcription factors as driver regulators (4 = present in all four datasets as driver regulon, 1 = present in a single dataset). (B-D’’) Transverse sections of an E12.5 Myf5Cre/+; Rosa26TdTomato/+; PdgfraH2BGFP/+ embryo immunostained for Foxp2 at the level of the EOM (B-B’’), Mandibular muscles (C-C’’), and Back muscles (D-D’’). Yellow arrowheads indicated the double positive cells to better appreciate Foxp2 intensity in Myf5-derived cells. (E) Quantification of Foxp2 signal intensity in TOM+ (Myf5-derived) cells in each muscle (n = 3 embryos). Statistical test performed: Mann-Whitney U test. (F) FACS plots of dissected E12.5 Myf5Cre/+; Rosa26TdTomato/+; PdgfraH2BGFP/+ embryos (head region here) highlighting the Myf5-derived GFP- TOM+ population transitioning to the GFP+ TOM+ population. Each plot was generated on the population gated in the previous one (‘Singlets’, ‘TOM+’ and ‘NonFaps’). FAPS:Fibroadipogenic progenitors, a denomination for resident Pdgfra+ cells. (G) Quantification of the transitioning population in Head, Limb and Trunk. Proportion of transitioning cells is calculated as the number of Alexa488+/Total cell number in the ‘NonFAPs’ gate. Note that the Head region is mostly populated by muscles embedded in neural crest (n = 5 embryos). TOM: TdTOMATO.

Figure 7—source data 1

Excel table summarizing the quantification displayed on Figure 7E.

https://cdn.elifesciences.org/articles/70235/elife-70235-fig7-data1-v3.xlsx
Figure 7—source data 2

Excel table summarizing the quantification displayed on Figure 7G.

https://cdn.elifesciences.org/articles/70235/elife-70235-fig7-data2-v3.xlsx
Table 2
Driver regulators of non-myogenic fate in each dataset.
E10.5E11.5E12.5E14.5
Foxp2(+)(+)(+)(+)
Hmga2(+)(+)(+)(+)
Meis1(+)(+)(+)(+)
Meox2(+)(+)(+)(+)
Tcf7l2(+)(+)(+)(+)
Fli1(+)(+)(+)(-)
Lef1(-)(+)(+)(+)
Prrx1(+)(+)(-)(+)
Prrx2(-)(+)(+)(+)
Six2(+)(+)(+)(-)
Creb3l1(-)(+)(-)(+)
Ebf1(+)(-)(+)(-)
Ets1(-)(+)(-)(+)
Foxp4(+)(+)(-)(-)
Hoxb3(-)(+)(+)(-)
Klf6(-)(+)(-)(+)
Nfatc4(-)(+)(-)(+)
Nfib(-)(+)(+)(-)
Pax7(-)(-)(+)(+)
Pbx1(-)(+)(-)(+)
Rreb1(-)(-)(+)(+)
Tbx15(+)(+)(-)(-)
Tcf4(+)(-)(+)(-)
Twist1(+)(+)(-)(-)
Zic4(+)(-)(+)(-)
Zmiz1(-)(+)(+)(-)
Ar(-)(-)(-)(+)
Arid5b(-)(-)(+)(-)
Atf3(-)(-)(-)(+)
Chd2(+)(-)(-)(-)

  1. (+): Present, (-): Absent.

Forkhead box transcription factors FOXC1 and FOXC2 were reported to regulate the balance between myogenic and vascular lineages within somites (Lagha et al., 2009; Mayeuf-Louchart et al., 2016). Interestingly, Foxc1 has been reported to promote both cranial vasculature and cranial cartilage development in zebrafish (Whitesell et al., 2019; Xu et al., 2021). FOXP2 immunostaining on Myf5Cre/+;Rosa26TdTom/+;PdgfraH2BGFP/+ E12.5 embryos showed that the Myf5-derived EOM cells expressed a relatively high level of Foxp2 compared to mandible and trunk muscles, consistent with their apparent high contribution to connective tissue (Figure 7B–E).

To gain further insights into the transitioning population, we performed FACS analysis of dissected head, limb and trunk regions of Myf5Cre/+;Rosa26TdTom/+;PdgfraH2BGFP/+ embryos at E12.5 (Figure 7F–G). We focused on TOM+ cells (Myf5-lineage) and assessed their GFP expression levels as a readout of their commitment toward connective tissue. This analysis identified non-FAPs cells (GFPlow) transitioning towards a Pdgfra+ state in head and trunk regions but very few in the limb (Figure 7G). Interestingly, while trunk muscles presented the largest portion of transitioning cells (40%), a similar transitioning population was noted in the head (20%) despite a large contribution of NCC to head connective tissues. Thus, cardiopharyngeal mesoderm may have a superior potential to give rise to connective tissue compared to somite-derived progenitors in the limb (1.5%).

In addition, Tcfs and Lef1 were among the top common regulators identified, and they form a complex effector for the canonical Wnt pathway. Previous work showed that during cranial myogenesis, neural crest cells release inhibitors of the Wnt pathway to promote myogenesis (Tzahor et al., 2003). It is thus tempting to speculate that in the absence of neural crest, mesoderm-derived progenitors can give rise to connective tissue by maintaining canonical Wnt activity. To test this hypothesis, we examined the expression of Axin2, a common readout for Wnt/β-cat activity (Babb et al., 2017; van de Moosdijk et al., 2020). Interestingly, Axin2 levels were elevated in the non-myogenic portion of all the different datasets (Figure 7—figure supplement 1E-H). Additionally, Dkk2, which has been described as an activator of Wnt/β-cat pathway in the neural crest (Devotta et al., 2018), was also found to be elevated, indicative of a putative positive-feedback loop mechanism supporting the maintenance of this population.

Discussion

Distinct fates can emerge through the specification of individual cell types, or through direct lineage ancestry from bipotent or multipotent cells. Here, we addressed this issue in the context of the emergence of myogenic and associated connective tissue cells during the formation of craniofacial muscles. By combining state-the-art computational methods and in-situ analyses, we identified the transcriptional dynamics, the intercellular communication networks, and the regulators controlling the balance between complementary cell fates. Specifically, our work provides evidence for a novel mesoderm-derived bipotent cell population that gives rise to muscle and associated connective tissue cells spatiotemporally, and only in regions deprived of neural crest cells (Figure 7—figure supplement 2).

Brown adipocytes, neurons, pericytes, and rib cartilage have been reported to express Myf5 in ancestral cells (Daubas et al., 2000; Haldar et al., 2008; Sebo et al., 2018; Stuelsatz et al., 2014). Interestingly, when Myf5 expression is disrupted, cells can acquire non-myogenic fates and contribute to connective tissue (this study), cartilage, and dermis (Tajbakhsh et al., 1996), while others remain apparently undifferentiated (cells labeled with an asterisk in Figure 5C). It is likely that these cells are undergoing apoptosis as reported previously (Sambasivan et al., 2009). These studies suggest that Myf5-expression alone is not sufficient to promote robust myogenic fate in multiple regions of developing embryos. Consistent with these observations, Myod+ cells do not contribute to rib cartilage (Wood et al., 2020) and give rise to few connective tissue cells in the periocular and back regions (this study). These findings are also consistent with the role of Myod in defining the committed myogenic cell state and its higher chromatin-remodelling capacity compared to Myf5 (Conerly et al., 2016; Tapscott, 2005). In contrast to a previous study (Stuelsatz et al., 2014), we found no neural-crest derived cells expressing Myf5 during EOM tissue genesis at E13.5 (using Wnt1Cre/+;Rosa26mTmG/+;Myf5nlacZ/+). We note that Myf5-expressing cells contribute to non-myogenic cells from early embryonic stages (E10.5) and continue to do so in the fetus, indicating that these bipotent cells persist well after muscles are established.

Here, we also identifed a core set of transcription factors specifically active in the non-myogenic cells across all datasets. We propose that these genes guide bipotent cells to a non-myogenic fate and thus confer mesenchymal properties to non-committed progenitors. Recent studies have identified anatomically distinct fibroblastic populations using single-cell transcriptomics, yet unique markers could not be identified (Muhl et al., 2020; Sacchetti et al., 2016), making characterisation of cell subtypes challenging. Tcf4/Tcf7l2 was identified as a master regulator of fibroblastic fate during muscle-associated connective tissue development, although it is also expressed in myogenic progenitors at lower levels (Kardon et al., 2003; Mathew et al., 2011; Sefton and Kardon, 2019). We also report that this gene is one of the main regulators of connective tissue fate. Other transcription factors have been linked to skin fibroblast fates including Tcf4, Six2, Meox2, Egr2, and Foxs1, and their repression favors a myofibroblastic potential (Noizet et al., 2016). Six2 and Meox2 were also identified in our analysis, which raises the question of the shared genetic programs between myofibroblastic cells and fibroblastic cells derived from progenitors primed for myogenesis during development.

Interestingly, Prrx1, a marker for lateral plate mesoderm (Durland et al., 2008), was differentially expressed in the connective tissue population at various stages. Although lateral plate mesoderm is identifiable in the trunk, its anterior boundaries in the head are unclear (Prummel et al., 2020). More detailed analyses of Prrx1, Isl1, and Myf5 lineages need to be carried out to delineate the specific boundaries of each progenitor contribution to cranial connective tissues.

Kinase receptors have been implicated in a number of developmental programs for both muscle and associated connective tissues (Arnold et al., 2020; Knight and Kothary, 2011; Olson and Soriano, 2009; Tallquist et al., 2000; Tzahor et al., 2003; Vinagre et al., 2010). For example, the differentiation of fetal myoblasts is inhibited by growth factors Tgfβ and Bmp4 (Cossu et al., 2000). Epha7 signaling is active in embryonic and adult myocytes and promotes their differentiation (Arnold et al., 2020). Significantly, we noticed a striking and lasting complementary expression of Pdgfa and Pdgfra throughout embryonic stages, in the myogenic and non-myogenic progenitors respectively. Pdgf ligands emanating from hypaxial myogenic cells under the control of Myf5 were shown to be necessary from rib cartilage development (Tallquist et al., 2000; Vinagre et al., 2010). Additionally, Pdgfra promotes expansion of fibroblasts during fibrosis (Olson and Soriano, 2009). Interestingly, we found that Pdgfa expression was reduced in cells expressing high levels of Myog at the fetal stage (Figure 6—figure supplement 2C). Therefore, Myf5-derived myogenic progenitor cells might guide non-myogenic Myf5-derived expansion, which in turn provides ligands and extracellular matrix components to favor myogenic development and patterning. Moreover, unlike trunk myogenesis, cranial muscle development relies on the expression of Wnt and Bmp inhibitors from surrounding tissues (Tzahor et al., 2003). Interestingly, we showed that the Myf5-derived non-myogenic cells express Bmp4, Dkk2, and Axin2. Additionally, we showed that the Wnt effector complex Tcf/Lef is expressed to a lower extent in these cells. It is thus likely that these cells maintain their non-myogenic fate by promoting Bmp production and Wnt activity cell-autonomously.

Of note, another study suggested shared fate relationships between fibroblast connective tissue cells and skeletal muscle where fibroblastic cells commit to myogenic fate during limb development (Esteves de Lima et al., 2021). Regarding the possibility that some non-myogenic cells may retain bipotent characteristics, our data suggests that the opposite is true during cranial muscle development. First, RNA velocity analysis did not reveal transitioning cells from non-myogenic clusters to myogenic (even at early stages), nor do they express myogenic markers. Further, at least some of these non-myogenic cells gave rise to chondrocytes, which to our knowledge has never been shown to give rise to skeletal muscle. Additionally, bipotency appears to be more associated with myogenic cells since they express Myf5, and to a minor extent Myod. Finally, we did not observe NCC-derived Myf5+ cells indicating that connective tissue in the head does not give rise to muscle. Nevertheless, to formally exclude the possibility of connective tissue progenitors giving rise to muscle in the embryo, analysis of appropriate markers would need to be done (ex. Pdgfra-driven lineage). Further studies should provide insights into the evolutionary ancestry of progenitors that bifurcate to give rise to myogenic and connective tissue cells by studying other model organisms that are devoid of neural crest cells.

Materials and methods

Key resources table
Reagent type (species) or resourceDesignationSource or referenceIdentifiersAdditional information
Strain, strain background (Mus musculus)B6D2F1/JRjJanvier  
Genetic reagent (M. musculus)Myf5CrePMID:17418413MGI:3710099Dr. Mario R Capecchi (Institute of Human Genetics, University of Utah, USA)
Genetic reagent (M. musculus)Wnt1CrePMID:9843687MGI:J:69326Pr. Andrew P. McMahon (Keck School of Medicine of the University of Southern California, USA)
Genetic reagent (M. musculus)Mesp1CrePMID:10393122MGI:2176467Pr. Yumiko Saga (National Institute of Genetics, Japan)
Genetic reagent (M. musculus)Myf5nlacZPMID:8918877MGI:1857973Dr. Shahragim Tajbakhsh (Department of Developmental and Stem Cell Biology, Institut Pasteur, France)
Genetic reagent (M. musculus)Rosa26tdTomatoPMID:20023653MGI:3809524Dr. Hongkui Zeng (Allen Institute for Brain Science, USA)
Genetic reagent (M. musculus)Rosa26mT/mGPMID:17868096MGI:3716464Pr. Philippe Soriano (Icahn School of Medicine at Mt. Sinai, USA)
Genetic reagent (M. musculus)PdgfraH2BGFPPMID:12748302MGI:2663656Pr. Philippe Soriano (Icahn School of Medicine at Mt. Sinai, USA)
Genetic reagent (M. musculus)MyodiCrePMID:19464281MGI:3840216Pr. David Goldhamer (University of Connecticut, USA)
Genetic reagent (M. musculus)Myf5GFP-PPMID:15386014MGI:3055340Dr. Shahragim Tajbakhsh (Department of Developmental and Stem Cell Biology, Institut Pasteur, France)
Chemical compound, drugSucrose,for molecular biology, ≥ 99.5% (GC)Sigma-AldrichS0389-500G
Chemical compound, drugGelatinSigma-AldrichG-7041
AntibodyAnti-Foxp2 5C11A8 (Mouse monoclonal)Santa CruzSC-517261IF (1:200)
AntibodyAnti-β-gal (Chicken polyclonal)AbcamCat. #: ab9361 RRID:AB_307210IF (1:1000)
AntibodyAnti-β-gal (Rabbit polyclonal)MP BiomedicalsCat. #: MP 559761 RRID:AB_2687418IF (1:1500)
AntibodyAnti-GFP (Chicken polyclonal)Aves LabsCat. #: 1020 RRID:AB_10000240IF (1:500)
AntibodyAnti-GFP (Chicken polyclonal)AbcamCat. #: 13970 RRID:AB_300798IF (1:1000)
AntibodyAnti-Myod (Mouse monoclonal)DakoCat. #: M3512 RRID:AB_2148874IF (1:100)
AntibodyAnti-Myod (Mouse monoclonal)BD-BiosciencesCat. #: 554130 RRID:AB_395255IF (1:500)
AntibodyAnti-Pax7 (Mouse monoclonal)DSHBCat. #: Pax7 RRID:AB_528428IF (1:20)
AntibodyAnti-Myog (Mouse monoclonal)DSHBCat. #: F5D RRID:AB_2146602IF (1:20)
AntibodyAlexa Fluor 633 F(ab')2 Fragment of Goat Anti-Rabbit IgG (H + L) (polyclonal antibody)Life TechnologiesCat. #: A-21072 RRID:AB_2535733IF (1:500)
AntibodyAlexa Fluor 555 F(ab')2 Fragment of Goat Anti-Rabbit IgG (H + L) (polyclonal antibody)Life TechnologiesCat. #: A-21430 RRID:AB_2535851IF (1:500)
AntibodyAlexa Fluor 488 F(ab')2 Fragment of Goat Anti-Rabbit IgG (H + L) (polyclonal antibody)Life TechnologiesCat. #: A-11070 RRID:AB_2534114IF (1:500)
AntibodyAlexa Fluor 633 Goat Anti-Chicken IgG (H + L) (polyclonal antibody)Life TechnologiesCat. #: A-21103 RRID:AB_2535756IF (1:500)
AntibodyAlexa Fluor 488 Goat Anti-Chicken IgG (H + L) (polyclonal antibody)Life TechnologiesCat. #: A-11039 RRID:AB_2534096IF (1:500)
AntibodyAlexa Fluor 633 Goat Anti-Mouse IgG1 (γ1) (polyclonal antibody)Life TechnologiesCat. #: A-21126 RRID:AB_2535768IF (1:500)
AntibodyAlexa Fluor488 AffiniPure Goat Anti-Mouse IgG1 (γ1) (polyclonal antibody)Jackson ImmunoResearchCat. #: 115-545-205 RRID:AB_2338854IF (1:500)
AntibodyCy3-AffiniPure Goat Anti-Mouse IgG1 (γ1) (polyclonal antibody)Jackson ImmunoResearchCat. #: 115-165-205 RRID:AB_2338694IF (1:500)
AntibodyCy3-AffiniPure Goat Anti-Mouse IgG2a (γ2a) (polyclonal antibody)Jackson ImmunoResearchCat. #: 115-165-206 RRID:AB_2338695IF (1:500)
AntibodyDylight 405 Goat Anti-Mouse IgG2a (γ2a) (polyclonal antibody)Jackson ImmunoResearchCat. #: 115-475-206 RRID:AB_2338800IF (1:500)
Commercial assay, kitHoechst 33,342Thermo ScientificCat. #:H3570 
Commercial assay, kitRNAscope Multiplex Fluorescent reagent Kit-V2ACD/Bio-techneCat. #: 323100 
Commercial assay, kitRNAscope H202 & Protease Plus ReagentsACD/Bio-techneCat #: 322330 
Commercial assay, kitOpal 650 Reagent PackPerkinElmerCat. #: FP1496001KT1:1,500 of reconstituted reagent in RNAscope Multiplex TSA Buffer
Commercial assay, kitOpal 570 Reagent PackPerkinElmerCat. #: FP1488001KT1:1,500 of reconstituted reagent in RNAscope Multiplex TSA Buffer
Commercial assay, kitRNAscope Mm-PdgfaAdvanced Cell Diagnostics, IncCat #:411361 
Commercial assay, kitRNAscope Mm-PdgfraAdvanced Cell Diagnostics, IncCat #:480661-C2 
Commercial assay, kitRNAscope Mm-Bmpr1bAdvanced Cell Diagnostics, IncCat #:533941 
Commercial assay, kitRNAscope Mm-Efnb1Advanced Cell Diagnostics, IncCat #:526761 
Commercial assay, kitRNAscope Mm-Bmp4-O1-C3Advanced Cell Diagnostics, IncCat #:527501-C3 
Commercial assay, kitRNAscope Mm-Ephb1-C3Advanced Cell Diagnostics, IncCat #:567571-C3 
Commercial assay, kitRNAscope Mm-Scube1Advanced Cell Diagnostics, IncCat #:488131 
Chemical compound, drugParaformaldehydeElectron Microscopy SciencesCat. #: 15710 
Chemical compound, drugIsopentaneVWRCat. #: 24872.298 
Chemical compound, drugTriton X-100SigmaCat. #: T8787 
Chemical compound, drugTween 20SigmaCat. #: P1379
Chemical compound, drugTrypLEThermoFisherCat #: 12604013
Chemical compound, drugCalcein BlueeBioscienceCat #: 65-0855-39
Chemical compound, drugPropidium IodideThermoFisherCat #: P1304MP
Commercial assay, kitChromium Next GEM Chip G Single Cell Kit, 16 rxns10 X GenomicsCat #: 1000127 
Commercial assay, kitChromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1, 4 rxns10 X GenomicsCat #:1000128 
Commercial assay, kitNextSeq 500/550 High Output Kit v2.5IlluminaCat #: 20024906 
Commercial assay, kitAgilent High Sensitivity DNA KitAgilentCat #:5067–4626 
Commercial assay, kitAgilent High Sensitivity DNA ReagentsAgilentCat #:5067–4627 
Commercial assay, kitQubit dsDNA HS Assay KitLife TechnologiesCat #:Q32854 
Software, algorithmRStudioRstudio  
Software, algorithmAnacondaAnaconda Inc  
Software, algorithmZenZeiss  
Software, algorithmCytoscapeCytoscape Team  
Software, algorithmFijiJohannes Schindelin, Ignacio Arganda-Carreras, Albert Cardona, Mark Longair, Benjamin Schmid, and others  
Software, algorithmPrismGraphPad Software  
Software, algorithmFlowJoFlowJo  

scRNAseq data generation

Request a detailed protocol

For E10.5 to E12.5 embryos, the cranial region above the forelimb was dissected in ice-cold 3% FBS in PBS and mechanically dissociated with forceps and pipetting. The same procedure was applied at E14.5 but the dissection was refined to the pharyngeal and laryngeal regions. Tissues were then digested in TrypLE (ThermoFisher Cat #: 12604013) during 3 rounds of 5 min incubation (37 °C, 1400 RPM), interspersed with gentle pipetting to further dissociate the tissue. Cells were resuspended in FBS 3%, filtered, and incubated with Calcein Blue (eBioscience, Cat #: 65-0855-39) and Propidium Iodide (ThermoFisher Cat #: P1304MP) to check for viability. Viable cells were sorted on BD FACS Aria III and manually counted using a hemocytometer. RNA integrity was assessed with Agilent Bioanalyzer 2,100 to validate the isolation protocol prior to scRNAseq (RIN >8 was considered acceptable). A total of 4000–13,000 cells were loaded onto 10 X Genomics Chromium microfluidic chip and cDNA libraries were generated following manufacturer’s protocol. Concentrations and fragment sizes were measured using Agilent Bioanalyzer and Invitrogen Qubit. cDNA libraries were sequenced using NextSeq 500 and High Output v2.5 (75 cycles) kits. Genome mapping and count matrix generation were done following 10X Genomics Cell Ranger pipeline.

RNA velocity and driver genes

Request a detailed protocol

RNA velocity analyses were performed using scvelo (Bergen et al., 2020) in Python. This tool allows inferring velocity flow and driver genes using scRNAseq data, with major improvements from previous methods (La Manno et al., 2018). First, unspliced and spliced transcript matrices were generated using velocyto (La Manno et al., 2018) command line function, which outputs unspliced, spliced, and ambiguous matrices as a single loom file. These files were combined with filtered Seurat objects to yield objects with unspliced and spliced matrices, as well as Seurat-generated annotations and cell-embeddings (UMAP, tSNE, PCA). These datasets were then processed following scvelo online guide and documentation. Velocity was calculated based on the dynamical model (using scv.tl.recover_dynamics(adata), and scv.tl.velocity(adata, mode=’dynamical’)) and when outliers were detected, differential kinetics based on top driver genes were calculated and added to the model (using scv.tl.velocity(adata, diff_kinetics = True)). Specific driver genes were identified by determining the top likelihood genes in the selected cluster. The lists of top 100 drivers for each stage are given in Table 1.

Data processing

Request a detailed protocol

scRNAseq datasets were preprocessed using Seurat in R (https://satijalab.org/seurat/) (Butler et al., 2018). Cells with more than 20% of mitochondrial gene fraction were discarded. The number of genes expressed averaged to 4000 in all four datasets. Dimension reduction and UMAP generation were performed following Seurat workflow. Doublets were inferred using DoubletFinder v3 (McGinnis et al., 2019). Cell cycle genes, mitochondrial fraction, number of genes, number of UMI were regressed in all datasets following Seurat dedicated vignette. We noticed that cell cycle regression, although clarifying anatomical diversity, seemed to induce low and high UMI clustering (Figure 4A, Figure 4—figure supplement 1C). For the E10.5 and E11.5 datasets, two replicates were generated from littermates and merged after confirming their similitude. For subsequent datasets (E12.5 and E14.5), no replicates were used. Annotation and subsetting were also performed in Seurat. ‘Myogenic’ and ‘Non-myogenic’ annotations were based on Pdgfa and Pdgfra expression and myogenic genes Myf5, Myod, and Myog. Cells not expressing Pdgfa were annotated as ‘non-myogenic’ unless they express myogenic genes. Cells expressing Pdgfa were annotated as ‘myogenic’. We noticed that at later stages, Pdgfa expression decreases in Myog+ cells. Driver genes of connective tissue at E12.5 and E14.5 were determined using cluster annotations obtained from Leiden-based clustering. Myogenic and non-myogenic scores were generated by aggregating the total expression of all genes in a signature based on the top 10 markers of these compartments (visible on Figure 4C). Each score was then divided by the sum of the two to generate myogenic and non-myogenic signatures. The coexpression score was defined by the product of these signatures. To generate the plots, cells were ordered based on their non-myogenic signature. The ‘transition’ was defined as cells with a coexpression score higher than 0.20.

Gene regulatory network inference

Request a detailed protocol

Gene regulatory networks were inferred using SCENIC (R implementation) (Aibar et al., 2017) and pySCENIC (Python implementation) (Van de Sande et al., 2020). This algorithm allows regrouping of sets of correlated genes into regulons (i.e. a transcription factor and its targets) based on motif binding and co-expression. UMAP and heatmap were generated using regulon AUC matrix (Area Under Curve) which refers to the activity level of each regulon in each cell. We used two cisTarget databases: ‘mm9-500bp-upstream-7species.mc9nr’ (500 bp upstream of TSS) and ‘mm9-tss-centered-10kb-7species.mc9nr’ (10kb ±TSS).

Driver regulons

Request a detailed protocol

Results from SCENIC and scvelo were combined to identify regulons that could be responsible for the transcriptomic induction of driver genes. Similarly to the steps mentioned above, SCENIC lists of regulons were used to infer connections between transcription factors and driver gene. Networks were generated as explained above and annotated with ‘Active regulon’ or ‘driver gene’. The lists of individual driver regulons of each dataset were then combined and the most recurring driver regulons were identified. The code is available at this address: https://github.com/TajbakhshLab/DriverRegulators, (copy archived at swh:1:rev:49db57e7ede9f248de937b7a47eb96b02aa2ce67; Grimaldi, 2021).

Gene set enrichment analysis

Request a detailed protocol

Gene set enrichment analyses were performed on either the top markers (obtained from Seurat function FindAllMarkers) or from driver genes (obtained from scvelo), using Cluego (Bindea et al., 2009). ‘GO Molecular Pathway’, ‘GO Biological Process’ and ‘Reactome pathways’ were used independently to identify common and unique pathways involved in each dataset. In all analyses, an enrichment/depletion two-sided hypergeometric test was performed and p-values were corrected using the Bonferroni step down method.

Mouse strains

Request a detailed protocol

Animals were handled as per European Community guidelines and the ethics committee of the Institut Pasteur (CETEA) approved protocols (APAFIS#6354–20160809 l2028839). The following strains were previously described: Myf5Cre (Haldar et al., 2008), MyodiCre (Kanisicak et al., 2009), Mesp1Cre (Saga et al., 1999), Tg:Wnt1Cre (Danielian et al., 1998), Rosa26TdTom (Ai9; Madisen et al., 2010), Rosa26mTmG (Muzumdar et al., 2007), Myf5nlacZ (Tajbakhsh et al., 1996), PdgfraH2BGFP (Hamilton et al., 2003) and Myf5GFP-P (Kassar-Duchossoy et al., 2004). To generate Myf5Cre/+;Rosa26TdTomato/+;PdgfraH2BGFP/+embryos, Myf5Cre/+ females were crossed with PdgfraH2BGFP/+;Rosa26TdTomato/TdTomato males. Mice were kept on a mixed genetic background C57BL/6JRj and DBA/2JRj (B6D2F1, Janvier Labs). Mouse embryos and fetuses were collected between embryonic day (E) E10.5 and E14.5, with noon on the day of the vaginal plug considered as E0.5.

Immunofluorescence

Request a detailed protocol

Collected embryonic and adult tissues were fixed 2.5 h in 4% paraformaldehyde (Electron Microscopy Sciences, Cat #:15710) in PBS with 0.2–0.5% Triton X-100 (according to their stage) at 4 °C and washed overnight at 4 °C in PBS. In preparation for cryosectioning, embryos were equilibrated in 30% sucrose in PBS overnight at 4 °C and embedded in OCT. Cryosections (16–20 µm) were left to dry at RT for 30 min and washed in PBS. For Foxp2 immunostaining (Santa Cruz Cat. #: SC-517261), embryos were first equilibrated in 15% sucrose overnight, then in a 15% sucrose/7.5% gelatin solution at 37 °C the next day and embedded in the same solution the following day. Blocks were then kept at 4 °C in a humid environment and trimmed, before being submerged in liquid nitrogen-cooled isopentane at –60 °C to freeze. After cryosectioning, slides were washed twice for 15 min each at 37 °C inPBS to remove the gelatin. The primary antibodies used in this study are chicken polyclonal anti-β-gal (Abcam, Cat #: ab9361, dilution 1:1000), mouse monoclonal IgG1 anti-Myod (BD Biosciences, Cat# 554130, dilution 1:100), mouse monoclonal IgG1 anti-Pax7 (DSHB, Cat. #: AB_528428, dilution 1:20), rabbit anti-mouse Sox9 (Millipore, Cat. #: AB5535, dilution 1/2000), rabbit polyclonal anti-Tomato (Clontech Cat. #: 632496, dilution 1:400) and chicken polyclonal anti-GFP (Abcam Cat. #: 13970, dilution 1:1000). Images were acquired using Zeiss LSM780 or LSM700 confocal microscopes and processed using ZEN software (Carl Zeiss). Control and mutant embryos were selected randomly, quantifications were performed blindly by hiding the discriminating channels. Quantifications were performed using Fiji (https://imagej.net/software/fiji/). Barplots, dotplots and boxplots were generated using Seaborn (https://seaborn.pydata.org; https://seaborn.pydata.org/) or Prism (https://www.graphpad.com/scientific-software/prism/). For the Myf5Cre/+;Rosa26TdTomato/+;PdgfraH2BGFP/+ embryos, 4 regions were manually defined across the medio-lateral axis. For each region, the absolute number of double-positive cells within the defined area was divided by the total number of GFP+ cells which was determined first, and blindly (with the TOMchannel disabled). For the MyodiCre lineage-tracing experiment, the absolute number of double positive cells were counted and divided by the area of the muscle given by the TOM channel. These ‘number of cells/area’ scores were then corrected based on the size of the image in microns, and adjusted to match an area of 100 μm2 of muscle. To quantify the intensity of Foxp2 immunostaining, we first generated ROIs of the Myf5-derived cells based on the TOM channels as previously mentioned and extracted the mean pixel value. All images were acquired using the exact same settings for a given embryo.

RNAscope in situ hybridization

Request a detailed protocol

Embryos for in situ hybridization were fixed overnight in 4% PFA. Embryos were equilibrated in 30% sucrose in PBS and sectioned as described for immunofluorescence. RNAscope probes Mm-Pdgfa (411361), Mm-Pdgfra (480661-C2), Mm-Bmpr1b (533941), Mm-Efnb1 (526761), Mm-Bmp4-O1-C3 (527501-C3), Mm-Ephb1-C3 (567571-C3) and Mm-Scube1 (488131) were purchased from Advanced Cell Diagnostics, Inc. In situ hybridization was performed using the RNAscope Multiplex Fluorescent Reagent Kit V2 as described previously (Comai et al., 2019). Quantifications were performed using Fiji (https://imagej.net/software/fiji/). 2 ROIs were first defined visually using the GFP channel: ‘myogenic’ and ‘non-myogenic’. The channels containing the RNAscope signals were then thresholded to obtain binary images, and measurement of the ‘Area%’ was performed for each ROI. For each probe, we generated a ratio of myogenic to non-myogenic signal. The ratio of each receptor was then substracted from the ratio of each corresponding ligand.

Data availability

scRNAseq datasets are available in open access on DRYAD at the following address: https://datadryad.org/stash/dataset/doi:10.5061/dryad.gf1vhhmrs?. The code that was used to generate the driver regulators is available at this address: https://github.com/TajbakhshLab/DriverRegulators, (copy archived at swh:1:rev:49db57e7ede9f248de937b7a47eb96b02aa2ce67). Source data files have been provided for Figure 3J, Figure 4H, Figure 5F, Figure 5J, Figure 5-figure supplement 1E, Figure 7E and Figure 7G.

The following data sets were generated

References

    1. Cossu G
    2. Angelis LD
    3. Borello U
    4. Berarducci B
    5. Buffa V
    6. Sonnino C
    7. Coletta M
    8. Vivarelli E
    9. Bouche M
    10. Lattanzi L
    11. Tosoni D
    12. Donna SD
    13. Berghella L
    14. Salvatori G
    15. Murphy P
    16. Angelis M
    17. Molinaro M
    (2000)
    Determination, diversification and multipotency of mammalian myogenic cells
    Int J Dev Biology 44:699–706.
    1. Shao X
    2. Wei X
    (2018)
    FOXP1 enhances fibrosis via activating Wnt/β-catenin signaling pathway in endometriosis
    American Journal of Translational Research 10:3610–3618.

Article and author information

Author details

  1. Alexandre Grimaldi

    1. Stem Cells and Development Unit, Institut Pasteur, Paris, France
    2. UMR CNRS 3738, Institut Pasteur, Paris, France
    3. Sorbonne Universités, Complexité du Vivant, F-75005, Paris, France
    Contribution
    Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Software, Supervision, Validation, Visualization, Writing – original draft, Writing – review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-5978-2057

  2. Glenda Comai

    1. Stem Cells and Development Unit, Institut Pasteur, Paris, France
    2. UMR CNRS 3738, Institut Pasteur, Paris, France
    Contribution
    Conceptualization, Formal analysis, Investigation, Validation, Visualization, Writing – review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-3244-3378

  3. Sebastien Mella

    1. Cytometry and Biomarkers UTechS, Institut Pasteur, Paris, France
    2. Bioinformatics and Biostatistics Hub, Institut Pasteur, Paris, France
    Contribution
    Data curation, Formal analysis, Software, Writing – review and editing
    Competing interests
    No competing interests declared
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-8679-5718

  4. Shahragim Tajbakhsh

    1. Stem Cells and Development Unit, Institut Pasteur, Paris, France
    2. UMR CNRS 3738, Institut Pasteur, Paris, France
    Contribution
    Conceptualization, Funding acquisition, Project administration, Resources, Supervision, Writing – review and editing
    For correspondence
    shahragim.tajbakhsh@pasteur.fr
    Competing interests
    Reviewing editor, eLife
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-1809-7202

Funding

Association Française contre les Myopathies (20510)

  • Alexandre Grimaldi

Fondation pour la Recherche Médicale (FDT201904008277)

  • Alexandre Grimaldi

Agence Nationale de la Recherche (ANR-10-LABX-73)

  • Shahragim Tajbakhsh

Centre National de la Recherche Scientifique

  • Glenda Comai

Agence Nationale de la Recherche (ANR-19-CE13-0008-01)

  • Shahragim Tajbakhsh

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Acknowledgements

We acknowledge funding support from the Institut Pasteur, Association Française contre le Myopathies, Agence Nationale de la Recherche (Laboratoire d’Excellence Revive, Investissement d’Avenir; ANR-10-LABX-73) and MyoHead (ANR-19-CE13-0008-01), Association Française contre les Myopathies (Grant #20510), Fondation pour la Recherche Médicale (Grant # FDT201904008277), and the Centre National de la Recherche Scientifique. We gratefully acknowledge the UtechS Photonic BioImaging, C2RT, Institut Pasteur, supported by the French National Research Agency (France BioImaging; ANR-10–INSB–04; Investments for the Future).

Ethics

Animals were handled as per European Community guidelines and the ethics committee of the Institut Pasteur (CETEA) approved protocols (APAFIS#6354-20160809 l2028839).

Copyright

© 2022, Grimaldi et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

Metrics

  • 3,354
    views
  • 472
    downloads
  • 14
    citations

Views, downloads and citations are aggregated across all versions of this paper published by eLife.

Download links

A two-part list of links to download the article, or parts of the article, in various formats.

Downloads (link to download the article as PDF)

Open citations (links to open the citations from this article in various online reference manager services)

Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)

  1. Alexandre Grimaldi
  2. Glenda Comai
  3. Sebastien Mella
  4. Shahragim Tajbakhsh
(2022)
Identification of bipotent progenitors that give rise to myogenic and connective tissues in mouse
eLife 11:e70235.
https://doi.org/10.7554/eLife.70235

Share this article

https://doi.org/10.7554/eLife.70235

Categories and tags

Research organism

Further reading

    1. Developmental Biology

    Extramacrochaetae regulates Notch signaling in the Drosophila eye through non-apoptotic caspase activity

    Sudershana Nair, Nicholas E Baker
    Research Article

    Many cell fate decisions are determined transcriptionally. Accordingly, some fate specification is prevented by Inhibitor of DNA-binding (Id) proteins that interfere with DNA binding by master regulatory transcription factors. We show that the Drosophila Id protein Extra macrochaetae (Emc) also affects developmental decisions by regulating caspase activity. Emc, which prevents proneural bHLH transcription factors from specifying neural cell fate, also prevents homodimerization of another bHLH protein, Daughterless (Da), and thereby maintains expression of the Death-Associated Inhibitor of Apoptosis (diap1) gene. Accordingly, we found that multiple effects of emc mutations on cell growth and on eye development were all caused by activation of caspases. These effects included acceleration of the morphogenetic furrow, failure of R7 photoreceptor cell specification, and delayed differentiation of non-neuronal cone cells. Within emc mutant clones, Notch signaling was elevated in the morphogenetic furrow, increasing morphogenetic furrow speed. This was associated with caspase-dependent increase in levels of Delta protein, the transmembrane ligand for Notch. Posterior to the morphogenetic furrow, elevated Delta cis-inhibited Notch signaling that was required for R7 specification and cone cell differentiation. Growth inhibition of emc mutant clones in wing imaginal discs also depended on caspases. Thus, emc mutations reveal the importance of restraining caspase activity even in non-apoptotic cells to prevent abnormal development, in the Drosophila eye through effects on Notch signaling.

    1. Developmental Biology

    Motor neurons are dispensable for the assembly of a sensorimotor circuit for gaze stabilization

    Dena Goldblatt, Basak Rosti ... David Schoppik
    Research Article

    Sensorimotor reflex circuits engage distinct neuronal subtypes, defined by precise connectivity, to transform sensation into compensatory behavior. Whether and how motor neuron populations specify the subtype fate and/or sensory connectivity of their pre-motor partners remains controversial. Here, we discovered that motor neurons are dispensable for proper connectivity in the vestibular reflex circuit that stabilizes gaze. We first measured activity following vestibular sensation in pre-motor projection neurons after constitutive loss of their extraocular motor neuron partners. We observed normal responses and topography indicative of unchanged functional connectivity between sensory neurons and projection neurons. Next, we show that projection neurons remain anatomically and molecularly poised to connect appropriately with their downstream partners. Lastly, we show that the transcriptional signatures that typify projection neurons develop independently of motor partners. Our findings comprehensively overturn a long-standing model: that connectivity in the circuit for gaze stabilization is retrogradely determined by motor partner-derived signals. By defining the contribution of motor neurons to specification of an archetypal sensorimotor circuit, our work speaks to comparable processes in the spinal cord and advances our understanding of principles of neural development.

    1. Developmental Biology

    Reorganization of the flagellum scaffolding induces a sperm standstill during fertilization

    Martina Jabloñski, Guillermina M Luque ... Mariano G Buffone
    Research Article

    Mammalian sperm delve into the female reproductive tract to fertilize the female gamete. The available information about how sperm regulate their motility during the final journey to the fertilization site is extremely limited. In this work, we investigated the structural and functional changes in the sperm flagellum after acrosomal exocytosis (AE) and during the interaction with the eggs. The evidence demonstrates that the double helix actin network surrounding the mitochondrial sheath of the midpiece undergoes structural changes prior to the motility cessation. This structural modification is accompanied by a decrease in diameter of the midpiece and is driven by intracellular calcium changes that occur concomitant with a reorganization of the actin helicoidal cortex. Midpiece contraction occurs in a subset of cells that undergo AE, and live-cell imaging during in vitro fertilization showed that the midpiece contraction is required for motility cessation after fusion is initiated. These findings provide the first evidence of the F-actin network’s role in regulating sperm motility, adapting its function to meet specific cellular requirements during fertilization, and highlighting the broader significance of understanding sperm motility.