We selected two nanobody/NanoTag pairs to test in Drosophila, NbVHH05/VHH05-tag and Nb127D01/127D01-tag. NbVHH05 is a 111 amino acid (aa) nanobody that recognizes a 14 aa sequence (VHH05-tag, QADQEAKELARQIS) derived from the human E2 ubiquitin-conjugating enzyme UBC6e (Figure 1A and B), and has a binding constant (K_d) for the VHH05-tag of ~0.15 nM (Ling et al., 2019). Nb127D01 is a 115 aa nanobody that binds to an extracellular portion of the human C-X-C chemokine receptor type 2 (CXCR2) (Bradley et al., 2015). As the extracellular region of CXCR2 is large, we reduced the epitope-binding region to a minimal sequence of 10 aa (127D01-tag, SFEDFWKGED) (Figure 1C and D; Figure 1—figure supplement 1) to obtain a more versatile tag, and determined the K_d of Nb127D01 to this tag to be <50 nM (data not shown). Protein-protein BLAST searches with both NanoTags failed to identify fully homologous sequences in the Drosophila proteome, thus reducing the possibility of spurious cross-reactions with endogenous, untagged proteins.

Figure 1 with 2 supplements see all

Download asset Open asset

To determine whether these NanoTags can be used for live imaging, we visualized both the NanoTags and their corresponding nanobodies concurrently. To do this, we constructed vectors that use the Actin5c promoter to ubiquitously express mCherry proteins equipped with the NanoTags and with different cell compartment localization sequences at the N-terminus: a cell membrane localization protein (murine CD8 gene, NM_009857.1), a mitochondrial outer membrane sequence (TM domain of Homo sapiens CDGSH iron sulfur domain 1, NM_018464.5), and a nuclear localization sequence (histone H2B gene, NM_001032214.2). We also constructed vectors that ubiquitously express GFP-tagged nanobodies (NbVHH05-GFP and Nb127D01-GFP) (Figure 1—figure supplement 2A, B) under the control of the Actin5c promoter.

When expressed alone in Drosophila S2R+ cells, NanoTagged mCherry fusion proteins were observed in the expected subcellular compartments Figure 1—figure supplement 2C, D), indicating that the VHH05- and 127D01-tags do not affect protein localization. When either NbVHH05-GFP or Nb127D01-GFP was expressed in cells, we observed a GFP signal in the nucleus and the cytoplasm, although some S2R+ cells transfected with Nb127D01-GFP contained aggregates (Figure 1—figure supplement 2C, D). Next, we co-transfected S2R+ cells with either mCherry-VHH05 fusion proteins and NbVHH05-GFP or mCherry-127D01 fusion proteins and Nb127D01-GFP. In all cases, the GFP and mCherry signals co-localized - with a distribution indistinguishable from that of mCherry fusions alone (Figure 1—figure supplement 2E, F). We also tested whether mCherry-tagged nanobodies (NbVHH05-mCherry or Nb127D01-mCherry) co-localized with mitochondrial GFP tagged with the VHH05-tag or the 127D01-tag, respectively. As expected, we observed co-localization of NbVHH05-mCherry and mito-GFP-VHH05 when expression vectors for each were co-transfected in S2R+ cells (Figure 1—figure supplement 2G. Similarly, Nb127D01-mCherry and mito-GFP-127D01 co-localized with mitochondria (Figure 1—figure supplement 2H).

We next examined whether the position of the NanoTags affects recognition by the nanobodies. We generated H2B-mCherry, mito-mCherry, and CD8-mCherry with N-terminal, internal, or C-terminal NanoTags. When these vectors were co-transfected with NbVHH05-GFP or Nb127D01-GFP in S2R+ cells, GFP and mCherry co-localized with the expected cellular compartments (Figure 1E–J). Taken together, our data show that both NbVHH05 and Nb127D01 can be used as CBs to visualize and monitor NanoTagged proteins in their native surroundings.

In addition to establishing CB-based detection of NanoTagged proteins in cells, we also explored detection by immunofluorescence. Nanobodies can be detected by either direct or indirect immunofluorescence. Direct immunofluorescence involves the use of fluorophore-conjugated nanobodies to detect the target protein(s). Indirect immunofluorescence involves recognition of the target by the nanobody, followed by detection of the nanobody recognized by a secondary fluorophore-conjugated antibody.

For direct immunofluorescence, we chemically conjugated NbVHH05 and Nb127D01 with fluorophores (NbVHH05-CF555 and Nb127D01-CF647). The success of these conjugations was confirmed by the detection of a fluorescent signal on an SDS-PAGE gel (Figure 2—figure supplement 1). Immunostaining with these fluorophore-conjugated nanobodies directly revealed VHH05- and 127D01-tagged proteins in S2R+ cells (Figure 2A and A’; Figure 2—figure supplement 2A1, A2 ). Direct immunostaining with fluorophore-conjugated nanobodies thus provides a simple and efficient method of detection. The reactivity of neither NbVHH05 nor Nb127D01 was affected by direct chemical conjugation. In addition, we used a fluorescent NbVHH05-AF555 prepared by site-specific Sortase labeling (Figure 2—figure supplement 2A3). For C-terminal Sortase-mediated labeling, the Sortase recognition motif (LPETG) is added to the C-terminus of the nanobody. Next, catalyzed by the Sortase, a fluorophore or biotin is added, using modified oligoglycine peptides such as GGG-fluorophore or GGG-biotin, to the nanobody-LPETG (Cheloha et al., 2019). These chemoenzymatic reactions proceed near-quantitatively and are highly site-specific. Unlike chemical modification, the sortase labeling procedure does not entail the risk of unwanted side reactions that might otherwise affect the physicochemical properties of the final product. Because each enzymatically modified nanobody carries a single substituent (fluorophore or biotin), quantitative comparisons are in principle possible, which would be more challenging when using direct chemical conjugation.

Figure 2 with 3 supplements see all

Download asset Open asset

To test NbVHH05 and Nb127D01 in indirect immunofluorescence, we prepared bacterially purified NbVHH05 and Nb127D01, each fused with an ALFA-tag or HA-tag. We also prepared conditioned media that contained NbVHH05 or Nb127D01 tagged with the Fc portion of human IgG (hIgG) (Figure 2—figure supplement 2B1). Likewise, we could visualize NbVHH05-hIgG and Nb127D01-hIgG using fluorescently labeled anti-hIgG antibodies (Figure 2—figure supplement 2B1). Consistent with a previous report (Götzke et al., 2019), NbVHH05-ALFA and Nb127D01-ALFA can be visualized using anti-ALFA nanobodies conjugated to fluorophores (Figure 2; Figure 2—figure supplement 2B2). We could visualize NbVHH05-HA and Nb127D01-HA using fluorescently labeled anti-HA antibodies (Figure 2—figure supplement 2B3). Next, we determined whether ALFA-, HA-, or hIgG-tagged NbVHH05 and Nb127D01 could be used as the primary reagents for indirect immunostaining. We stained S2R+ cells transfected with mito-GFP-VHH05 or mito-GFP-127D01 vectors with the corresponding primary and secondary antibodies. In both cases, the GFP signal overlapped completely with the fluorescent signal from the secondary antibody (Figure 2B’; Figure 2—figure supplement 2B). NbVHH05 and Nb127D01 were obtained by immunization of an alpaca and llama, respectively (Ling et al., 2019; Bradley et al., 2015). We therefore examined whether they are both recognized by fluorophore-conjugated anti-alpaca IgG 647 which is also reactive with llama-derived nanobodies (or the VHH domain of llama IgG) (Figure 2C). The NanoTagged GFP and 647-fluorophore signals overlapped completely (Figure 2C’; Figure 2—figure supplement 2B4), indicating that commercially available secondary antibodies against llama are compatible with NbVHH05 and Nb127D01 immunostaining. In addition, we carried out indirect immunofluorescence using NbVHH05-biotin prepared by sortase labeling (Cheloha et al., 2019) and obtained similar results (Figure 2—figure supplement 2B5).

The availability of two different NanoTag-nanobody pairs opens the possibility for co-staining or co-detection with CBs. To test this, we generated proteins tagged with both NanoTags (VHH05- and 127D01-tags) and used NbVHH05 and Nb127D01 fused to HA-tag or ALFA-tag with corresponding secondary antibodies for detection. Importantly, these two tagging systems operate orthogonally, as no co-localization signal was observed in cells transfected with 127D01-GFP and with H2B-mCherry-VHH05 or mito-mCherry-VHH05 (VHH05-GFP with H2B-mCherry-127D01 or mito-mCherry-127D01) (Figure 2—figure supplement 3A). To further test co-detection, we inserted VHH05 at the N-terminus of the transcription factor REPTOR and 127D01 at its C-terminus (VHH05-REPTOR-127D01). When S2R+ cells were transfected with VHH05-REPTOR-127D01, the NbVHH05-HA signal (488-fluorophore) and the Nb127D01-ALFA signal (647-fluorophore) completely overlapped (Figure 2D and D’). Our data show that immunostaining using both of the NanoTag-nanobody pairs can be multiplexed.

Because the two nanobodies recognize small linear epitopes, we anticipated that these nanobodies might be useful for immunoblotting under denaturing conditions as already shown for VHH05 (Ling et al., 2019). We performed immunoblotting experiments with cell lysates containing VHH05- or 127D01-tagged H2B-mCherry. Using NbVHH05-ALFA and Nb127D01-ALFA as primary nanobodies, we detected a signal using NbALFA-HRP as the secondary antibody. Using purified nanobodies at high concentrations- produced some non-specific bands. This issue was resolved by reducing the nanobody concentration - (Figure 3—figure supplement 1). Next, we tested whether the two nanobodies could detect by immunoblotting proteins NanoTagged in different positions. NbVHH05 and Nb127D01 recognized proteins with internal, N- and C-terminal NanoTags on immunoblots (Figure 3A). To test whether increasing the number of NanoTags improved the sensitivity of detection, we generated vectors that express secreted GFP with 1x, 2x, or 3xVHH05 or 127D01 NanoTags at the C-terminus (Figure 3B). An N-terminal FLAG-tag was included in all constructs and used as the loading control. An increase in the number of NanoTags improved the sensitivity of detection using culture media that contain secreted GFP proteins (Figure 3B). Tagging of target proteins with more than one copy of a tag thus improves the sensitivity of detection.

Figure 3 with 2 supplements see all

Download asset Open asset

Figure 3—source data 1

Raw data of Western blot for Figure 3.

https://cdn.elifesciences.org/articles/74326/elife-74326-fig3-data1-v2.zip

Download elife-74326-fig3-data1-v2.zip

Next, we tested whether the nanobodies against the VHH05 and 127D01 tags could be used for multiplexed immunoblots by double-tagging the Upd2 cytokine (VHH05-Upd2-127D01). As we needed to detect each nanobody in a specific manner, we tagged one nanobody with the ALFA tag and detected it using NbALFA and the other, with an hIgG. We expressed and prepared nanobody-hIgG from S2 cells and tested different concentrations of conditioned media on immunoblots (Figure 3—figure supplement 2). Very diluted conditioned media still produced a strong signal, even though the nanobody-hIgG was not purified or concentrated. After establishing working concentrations of hIgG-tagged nanobody conditioned media, we performed multiplexed immunostaining using Nb127D01-hIgG and NbVHH05-ALFA, or NbVHH05-hIgG and Nb127D01-ALFA, detected with anti-hIgG and NbALFA, respectively (Figure 3C). Upd2 undergoes fragmentation due to internal furin cleavage sites, which produced different bands on the immunoblot. A combination of hIgG-tagged nanobody and ALFA-tagged nanobody can thus be used for multiplexed immunoblotting (Figure 3C). In addition, we confirmed that a combination of NbVHH05-biotin and Nb127D01-hIgG also worked well for multiplexed immunoblotting (Figure 3C).

Another key application of antibodies is immunopurification of target proteins. To explore whether NbVHH05 and Nb127D01 can be used for immunopurification, we coated NbALFA resin with ALFA-tagged NbVHH05 or Nb127D01 nanobodies and used the modified resin to recover NanoTagged FLAG-GFP secreted in S2 cell culture media. FLAG-GFP-3xVHH05 and FLAG-GFP-3x127D01 were captured by NbVHH05-ALFA and Nb127D01-ALFA, respectively (Figure 3D and E). Protein A magnetic beads coated with Nb127D01-hIgG also successfully recovered FLAG-GFP-3x127D01 from S2 cell culture media (Figure 3F). We confirmed that the VHH05 and 127D01 systems do not cross-react (Figure 2—figure supplement 3B). NanoTag-based immunopurification with these nanobodies is thus effective.

One of the many possible applications of nanobodies is to express them in cells or in vivo as fusion proteins localized to a particular subcellular location, in order to manipulate the localization of a NanoTagged POI. To test whether the NbVHH05/VHH05-tag and Nb127D01/127D01-tag can be used to alter localization, we constructed secreted GFP expression vectors that - had an N-terminal BiP signal peptide and a C-terminal VHH05- or 127D01-tag (BiP-GFP-VHH05 and BiP-GFP-127D01) (Figure 4A and B). We also constructed NbVHH05 and Nb127D01 with mCherry and KDEL endoplasmic reticulum (ER) retention signal (BiP-Nanobody-mCherry-KDEL), which should result in retention of the fusion protein in the ER (Figure 4A and B). When transfecting only BiP-GFP-VHH05 or BiP-GFP-127D01 into S2R+ cells, we did not observe GFP accumulation within cells, as the GFP proteins were actively secreted into the culture medium (Figure 4C). However, after co-transfecting BiP-NbVHH05-mCherry-KDEL with BiP-GFP-VHH05, or BiP-Nb127D01-mCherry-KDEL with BiP-GFP-127D01, we observed intracellular accumulation of GFP that co-localized with the mCherry signal. This shows that NanoTagged-GFP proteins directed to the secretory pathway can be trapped by an ER NanoTag trap (i.e. nanobodies with an ER retention signal) (Figure 4C). Indeed, cell lysates prepared from cells transfected with ER NanoTag trap showed more GFP signal on immunoblot, compared to controls lacking the ER NanoTag trap (Figure 4D).

Figure 4 with 1 supplement see all

Download asset Open asset

Figure 4—source data 1

Raw data of Western blot for Figure 4D.

https://cdn.elifesciences.org/articles/74326/elife-74326-fig4-data1-v2.zip

Download elife-74326-fig4-data1-v2.zip

We also prepared membrane-tethered nanobodies (membrane NanoTag trap) in order to re-localize cytoplasmic NanoTagged proteins to the membrane (Figure 4—figure supplement 1A, B). When S2R+ cells were co-transfected with CD8-NbVHH05-GFP/mito-mCherry-VHH05 or CD8-Nb127D01-GFP/mito-mCherry-127D01, mCherry co-localized with GFP on the cell membrane (Figure 4—figure supplement 1C). NanoTag traps targeted to particular cellular compartments can thus alter the subcellular distribution of NanoTagged proteins. The ability to do so may facilitate a variety of functional analyses.

The successful use of the NbVHH05/VHH05-tag and Nb127D01/127D01-tag in cells prompted us to test them in vivo. We first constructed a series of UAS vectors with either a cytoplasmic or secreted version of the two nanobodies, tagged with GFP or HA (Figure 5—figure supplement 1A), and tested them in cells. As expected, when transfected together with the pAct-Gal4 plasmid, the cytoplasmic expression vectors led to an expression of nanobodies detectable in S2R+ cell lysates, and the secretory expression vectors resulted in detection of nanobodies predominantly in the culture media (Figure 5—figure supplement 1B, C). Next, we generated transgenic flies carrying these UAS constructs. We did not expect NbVHH05 or Nb127D01 to interact with any fly endogenous proteins for lack of obvious sequence similarity between the Drosophila proteome and the amino acid sequence of the two tags (data not shown). To confirm this experimentally, we ubiquitously expressed NbVHH05 or Nb127D01 in vivo throughout development using tubulin-Gal4. The nanobodies were not toxic to flies; we readily obtained adult tubulin-Gal4, UAS-Nanobody flies and did not observe any detectable developmental defects or abnormalities (data not shown). To further test these constructs, we used fat body-specific Lpp-Gal4 to drive NbVHH05 or Nb127D01 expression. Immunofluorescence showed that GFP- or HA-tagged NbVHH05 or Nb127D01 is expressed at readily detectable levels in fat body cells (Figure 5A–D). We also confirmed that nanobodies with the BiP signal peptide are secreted (Figure 5E and F).

Figure 5 with 2 supplements see all

Download asset Open asset

Figure 5—source data 1

Raw data of Western blot for Figure 5E and F.

https://cdn.elifesciences.org/articles/74326/elife-74326-fig5-data1-v2.zip

Download elife-74326-fig5-data1-v2.zip

To address whether NanoTagged POIs can be detected in vivo, we generated UAS-VHH05-REPTOR-127D01 flies. A previous study has shown that in S2 cells, the transcription factor REPTOR is enriched in the cytoplasm under normal conditions but translocates into the nucleus upon rapamycin treatment (Tiebe et al., 2015). To test whether the changes in REPTOR localization can be detected using the nanobodies, we co-expressed NanoTagged REPTOR along with Nb127D01-GFP or NbVHH05-GFP specifically in adult enterocytes (ECs). In the absence of a NanoTagged POI, Nb127D01-GFP and NbVHH05-GFP were detected in both the cytoplasm and nuclei (Figure 5G1). In contrast, when co-expressed with NanoTagged REPTOR, the nanobody-GFP signals were enriched in the cytoplasm of ECs under normal food conditions (Figure 5H and J). Following rapamycin treatment, a stronger GFP signal was observed in nuclei. Similar changes were absent from ECs that express Nb127D01-GFP or NbVHH05-GFP alone (Figure 5G1). These results confirm that rapamycin treatment leads to translocation of REPTOR into the nucleus in vivo and provide further support for the idea that co-expression of NanoTagged POIs and nanobodies can visualize the subcellular location of a POI (Figure 5H and J).

Next, we checked whether NanoTagged proteins can be detected in vitro and in vivo using purified nanobodies. We first constructed several vectors that contain NanoTags at the N- or C-terminus of POIs and tested them in S2R+ cells. For secreted proteins, we replaced the endogenous signal peptide with the BiP signal peptide (Figure 5—figure supplement 1D). NanoTagged Akh, Dilp2, Dilp8, Pvf1, and Upd2 were readily detected in the culture media using NbVHH05 or Nb127D01 (Figure 5—figure supplement 1E). REPTOR and two isoforms of REPTOR-BP (REPTOR-BP-B and REPTOR-BP-C) could also be detected using NbVHH05 or Nb127D01 from transfected S2R+ cell lysates (Figure 5—figure supplement 1F, G). In addition to VHH05-REPTOR-127D01 flies, we also generated two additional transgenic flies: UAS-VHH05-REPTOR-BP-C-127D01 and UAS-BiP-VHH05-Upd2-127D01 (a version of Upd2 with the BiP secretion signal and both NanoTags). We used Myo1A^ts to drive UAS-VHH05-REPTOR-BP-C-127D01 and UAS-BiP-VHH05-Upd2-127D01 and were able to detect the signal in adult midguts, using either NbVHH05 or Nb127D01 for detection by immunofluorescence (Figure 5—figure supplement 2). Taken together, these data indicate that both NbVHH05/VHH05-tag and Nb127D01/127D01-tag work well for in vivo imaging and immunostaining.

In many cases, tagging endogenous proteins is preferable to UAS-based overexpression of tagged cDNAs, as UAS/Gal4-mediated expression can exceed physiological levels. Further, while many proteins have been tagged endogenously with GFP (Morin et al., 2001; Sarov et al., 2016; Li-Kroeger et al., 2018), tagging with smaller epitope tags may be preferable to minimize their structural impact. To tag endogenous genes with either VHH05-tag or 127D01-tag, we used a standard CRISPR-Cas9 targeted insertion method to tag fly proteins at their N- or C- terminus via the homology directed repair pathway (Figure 6—figure supplement 1A, B, C). To facilitate this approach, we first designed four universal vectors based on the scarless editing CRISPR knock-in (KI) approach (Lamb et al., 2017). Each vector contains five common features: the encoded NanoTags for N- or C-terminal tagging, 3xP3-dsRed-SV40 for identification of transformants, 5’/3’ terminal repeats for piggyBac transposase recognition sequences, TTAA for piggyBac target sequence, and an EcoRI restriction site for cloning target locus homologous arms by Gibson assembly (Figure 6—figure supplement 1A’). We chose histone H2A variant (H2Av) as an example, because the expected nuclear localization of H2Av should be easily visualized. We cloned the sequence 1 kb upstream and 1 kb downstream of the stop codon (TAA) into the donor vector (Figure 6—figure supplement 2A). A- sgRNA plasmid that targeted a seed sequence near the TAA of H2Av gene was injected into yw; nos-cas9/CyO embryos together with the donor plasmid (Figure 6—figure supplement 1B’). Positive transformants with red fluorescent eyes were outcrossed and successful KI events were confirmed by junction PCR and sequencing (Figure 6—figure supplement 1C’). Subsequently, 3xP3dsRed was excised using piggyBac transposase (Figure 6—figure supplement 2B). After sequence verification (Figure 6—figure supplement 2C), we immunostained midguts from H2Av-3x127D01 and H2Av-3xVHH05-expressing flies using Nb127D01-HA/NbVHH05-HA or NbVHH05-555/NbVHH05-biotin. As shown in Figure 6—figure supplement 1D’ and Figure 6—figure supplement 2D, H2Av tagged with either NanoTag was clearly observed in the nucleus, demonstrating that this tagging method can be used effectively to engineer NanoTagged forms of POIs and study their localization and/or function at physiological expression levels.

In order to further test the sensitivity and the possibility of labeling secreted small peptides by the KI method, we selected Dilp2 as an example. The above results showed that the double NanoTagged Dilp2 can be secreted into S2R+ culture medium and detected by western blot using NbVHH05 or Nb127D01 (Figure 5—figure supplement 1F). Here, we integrated 3x127D01 into the C-terminal of endogenous Dilp2 using KI strategy (Figure 6A, B, C). After confirming the proper integration (Figure 6D), we compared Nb127D01 and Dilp2 antibody stainings in the larval brain, which revealed similar expression patterns (Figure 6E; Park et al., 2014). Since introducing tags to proteins can negatively affect their functions and a previous study has shown that Dilp2 RNAi increases trehalose levels (Broughton et al., 2008), we examined the trehalose level of 127D01-tagged Dilp2 expressing flies. No difference was observed compared to control (Figure 6G), suggesting that that NanoTagged Dilp2 is functional. Furthermore, the change in Dilp2 signal in IPC cells and their neuronal projections in larval brain after starvation was easily detected by Nb127D01 immunostaining (Figure 6G). These results indicate that the CRISPR-mediated tagging of endogenous genes with NanoTags can be used for detecting small peptides with high sensitivity.

Figure 6 with 2 supplements see all

Download asset Open asset

Figure 6—source data 1

Raw data of agarose gel electrophoresis diagram for Figure 6B.

https://cdn.elifesciences.org/articles/74326/elife-74326-fig6-data1-v2.zip

Download elife-74326-fig6-data1-v2.zip

Four types of vectors were used in this study: (1) pAW, that contains the fly actin5C promoter; (2) pWalium10, a UAS/Gal4 vector that contains a UAS promoter and mini-white selection marker (DGRC, 1470); (3) pMT (pMK-33GW, Ram Viswanatha); and (4) pET-26b (Novagen 69862). pAW-HGP-sortase is a pAW vector derivative with N-terminal BiP signal peptide/FLAG-tag, AarI-cloning site for Gibson or HiFi assembly, C-terminal purification tag with Sortase-tag and His-tag, and heat shock promoter-GFP-T2A-PuroR for stable cell line generation. pMT-HGP-v3 is a pMT vector derivative with N-terminal BiP signal peptide/FLAG-tag, AarI-cloning site for Gibson or HiFi assembly, C-terminal purification tag with Avi-tag and His-tag, and heat shock promoter-GFP-T2A-PuroR for stable cell line generation. pET-26b-Nb-GGA is a pET-26b derivative with BsaI Golden Gate Assembly cloning site flanked by pelB signal sequence and C-terminal ALFA- and His-tags.

Plasmid DNAs were constructed and amplified using standard protocols. Briefly, plasmids were linearized by restriction enzymes as described by the commercial vendor. PCR fragments were amplified using Phusion polymerase (New England Biolabs [NEB], M0530) or Q5 polymerase (NEB, M0494). Linearized plasmids and PCR fragments were gel purified using QIAquick columns (Qiagen, #28115) and joined using either Gibson assembly (NEB, E2611) or NEBuilder HiFi assembly (NEB, E2621). Reactions were transformed into chemically competent TOP10 E. coli (Invitrogen, C404010), plated and selected on lysogeny broth-agar plates with ampicillin (100 μg/ml) or kanamycin (50 μg/ml). Colony PCR was performed using Takara Taq polymerase (Clontech, TAKR001C). Plasmid DNA was isolated from cultured bacteria using QIAprep Spin Miniprep Kit (Qiagen, 27104). Plasmid sequences were confirmed by Sanger sequencing performed at the Dana-Farber/Harvard Cancer Center DNA Resource Core or Genewiz. Primers sequences are listed in Supplementary file 1.

All UAS constructs were cloned into the pWalium10 (pW10) vector. For pW10-NbVHH05-HA, pW10-Nb127D01-HA, pW10-NbVHH05-GFP, pW10-Nb127D01-GFP, pW10-BiP-NbVHH05-HA, pW10-BiP-Nb127D01-HA, pW10-BiP-NbVHH05-GFP, and pW10-BiP-Nb127D01-GFP, pW10 was first linearized with EcoRI (NEB, R0101) and XbaI (NEB, R0145). NbVHH05 or Nb127D01 was cloned from pHEN6-VHH05 or pHEN6-127D01 (Ploegh lab). GFP was amplified from PXL-IE1-EGFP-nos-Cas9 (Xu et al., 2020). PCR fragments were joined together with the digested pW10 backbone by Gibson assembly. HA and BiP were incorporated by adding overhanging sequences to the primers. For pW10-BiP-VHH05-Akh-127D01, pW10-BiP-VHH05-Dilp2-127D01, pW10-BiP-VHH05-Dilp8-127D01, pW10-BiP-VHH05-Pvf1-127D01, and pW10-BiP-VHH05-Upd2-127D01, pW10 was first digested with EcoRI and XbaI. A BiP-VHH05-127D01 fragment was made by annealing two oligos and ligated into the linearized pW10 to generate the intermediate vector, pW10-BiP-VHH05-BgIII-127D01. Next, PCR fragments of Akh, Dilp2, Dilp8, Pvf1, and Upd2 were amplified from fly cDNAs and inserted into pW10-UAS-BiP-VHH05-127D01 linearized by BglII (NEB, R0144) by Gibson assembly. For pW10-127D01-REPTOR-bp-B-VHH05, pW10-127D01-REPTOR-bp-C-VHH05, and pW10-127D01-REPTOR-VHH05, pW10-BiP-VHH05-127D01 was digested with EcoRI and BglII to remove BiP-VHH05. VHH05-tag was introduced N-terminal of PCR fragments of REPTOR-bp-B, REPTOR-bp-C and REPTOR through overhanging primers. PCR fragments were inserted into pW10-127D01 (EcoRI and BglII digested) by Gibson assembly.

For pAW-NbVHH05-GFP, pAW-Nb127D01-GFP, pAW-NbVHH05-mCherry, pAW-Nb127D01-mCherry, pAW-H2B-mCherry-VHH05, pAW-H2B-mCherry-127D01, pAW-mito-mCherry-VHH05, pAW-mito-mCherry-127D01, pAW-CD8-mCherry-VHH05, pAW-CD8-mCherry-127D01, pAW-VHH05-H2B-mCherry, pAW-127D01-H2B-mCherry, pAW-BiP-NbVHH05-mCherry-KDEL, pAW-BiP-Nb127D01-mCherry-KDEL, pAW-CD8-NbVHH05-GFP, and pAW-CD8-Nb127D01-GFP, pAW was first linearized with NheI (NEB, R3131) and XbaI. NbVHH05-GFP and Nb127D01-GFP were amplified from pW10-NbVHH05-GFP and pW10-Nb127D01-GFP. CD8, mCherry, and CD8-mCherry were cloned from pQUASp-mCD8mCherry (Addgene, #46164). H2B-mCherry was cloned from pBac (3xP3-gTc’v; pUb:lox-mYFP-lox-H2BmCherry) (Addgene, #119064). mito-mCherry was cloned from pcDNA4TO-mito-mCherry-10xGCN4_v4 (Addgene, #60914). BiP-NbVHH05 or BiP-Nb127D01 fragments were cloned from pW10-UAS-BiP-NbVHH05-GFP, pW10-UAS-BiP-Nb127D01-GFP. PCR fragments were joined together with the digested pAW backbone by Gibson assembly. VHH05-, 127D01-tags, or KDEL sequences were incorporated in the N- or C-terminus by adding overhanging sequences to the primers. For pAW-CD8-VHH05-mCherry and pAW-CD8-127D01-mCherry, we first digested pAW-CD8-mCherry-VHH05 with NheI to obtain a linearized pAW-CD8 backbone. mCherry was amplified from pQUASp-mCD8mCherry. VHH05-tag or 127D01-tag was introduced into the N-terminal of PCR fragments with overhanging primer sequences. The NanoTag-mCherry PCR fragment was inserted into pAW-CD8 by Gibson assembly.

For pAW-HGP-BiP-GFP-VHH05, pAW-HGP-BiP-GFP-2xVHH05, pAW-HGP-BiP-GFP-3xVHH05, pAW-HGP-BiP-GFP-127D01, pAW-BiP-HGP-GFP-2x127D01, pAW-HGP-BiP-GFP-3x127D01, pAW-HGP-sortase was first linearized by AarI (Thermo Scientific ER1581). N-terminal GFP was amplified by PCR. C-terminal GFP with 1x/2x/3x NanoTags were prepared by gene synthesis (Twist Bioscience). PCR fragment and C-terminal GFP with NanoTags were joined together with the linearized pAW-HGP-sortase by NEBuilder HiFi assembly.

For cloning of the inducible Nb127D01-hIgG and NbVHH05-hIgG expression vectors, the pMT-HGP-v3 vector was linearized by both AarI and NsiI-HF (NEB, R3127). hIgG Fc region was amplified from pCER243 (a gift of Aaron Ring at Yale). NbVHH05/Nb127D01 and hIgG PCR fragments were inserted into pMT-HGP-v3 (digested by AarI and Nsil-HF) by NEBuilder HiFi assembly.

For cloning pET-26b-Nb127D01-ALFA-His and pET-26b-NbVHH05-ALFA-His, NbVHH05/Nb127D01 PCR fragments were cloned into pET-26b-Nb-GGA by BsaI-Golden Gate Assembly (NEB, E1601). To make pET-26b-Nb127D01-HA-His and pET-26b-NbVHH05-HA-His, NbVHH05/Nb127D01 PCR fragments with HA tag were cloned into pET-26b (NcoI, NEB, R3193 and XhoI, NEB, R0146 digested) by HiFi assembly.

3x127D01 or 3xVHH05 were cloned from pAW-HGP-BiP-GFP-3x127D01 or pAW-HGP-BiP-GFP-3xVHH05. 3xP3-DsRed was cloned from pScarlessHD-2xHA-DsRed (Addgene, 80822). Then, 3x127D01 or 3xVHH05 and 3xP3-DsRed PCR fragments were inserted into pScarlessHD-2xHA-DsRed backbone (EcoRI digested) by Gibson assembly.

pScarlessHD-C-3x127D01-DsRed and pScarlessHD-C-3xVHH05-DsRed were digested with EcoRI to obtain the pScarlessHD backbone and 3xP3-DsRed fragment. Upstream and downstream sequences of TAA at the C-terminus of the H2Av gene were cloned from yw; nos-Cas9/Cyo flies. Then, 3xP3-DsRed, upstream and downstream fragments were inserted into pScarlessHD backbone by Gibson assembly.

pCFD3 (Addgene, #49410) was digested with BbsI (NEB, R0539). sgRNA oligos following phosphorylation were annealed by T4PNK (NEB, M0201) and inserted into a digested pCFD3 backbone by T4 DNA ligase (NEB, M0202).

Drosophila S2R+ cells (DGRC, 150) were cultured at 25°C in Schneider’s media (Thermo Fisher Scientific, 21720–024) with 10% fetal bovine serum (Sigma, A3912) and 50 U/ml penicillin-streptomycin (Thermo Fisher Scientific, 15070–063). S2R+ cells were transfected using Effectene (Qiagen, 301427) following the manufacturer’s instructions. A total of 200 ng of plasmid DNA per well was transfected in 24-well plates. The culture medium was replaced 24 hr after transfection.

To produce secreted GFP proteins with 1x/2x/3x NanoTags and nanobodies with hIgG, ESF921-adapted S2 cells (Expression Systems, 94–005S) were transfected using PEI transfection methods and cultured in protein-free ESF921 media (Expression Systems, 96–001). For six-well plate transfection, 2.4 µg of DNA and 7.4 µg of PEI were mixed in 300 µl of ESF921 media for 15–30 min and added to 2.7 ml of S2 cell culture (2E6 cells/ml, final cell density). Alternatively, 50 ml suspension cells cultured in 250 ml flask were transfected using the same proportion. On day 2 from transfection, protein expression was induced with 700 µM CuSO4 for 5 days. Cleared conditioned media were collected after centrifugation and directly used for immunostaining, western blot, and immunoprecipitation.

Fly husbandry and crosses were performed under standard conditions at 25°C. Injections were carried in-house. Fly strains used to generate transgenic lines were attP lines: attP40 (y,v; P{nos- phiC31\int.NLS}X; P{CaryP}attP40) and attP2 (y,w; P{nos- phiC31\int.NLS}X; P{CaryP}attP2). Fly strains used to generate KI lines were y,w; nos-Cas9/CyO. For balancing chromosomes, fly stocks y,w; TM3, Sb/TM6,Tb, yw; Gla/CyO, yw; If/CyO; MKRS/TM6,Tb were used. All lines recovered were homozygous viable. Fly stocks are from the Perrimon fly stock unless stated otherwise. Other fly stocks used in this study were Lpp-Gal4 (Hong-Wen Tang) and Myo1A-Gal4, tub-Gal80^ts (Afroditi Petsakou).

• Transgenic flies generated in this study are as follows: yw; UAS-NbVHH05-HA, w + attP2 yw; UAS-NbVHH05-HA, w + attP40 yw; UAS-Nb127D01-HA, w + attP2

• yw; UAS-Nb127D01-HA, w+ attP40 yw; UAS-NbVHH05-GFP, w+ attP2

• yw; UAS-NbVHH05-GFP, w+ attP40 yw; UAS-Nb127D01-GFP, w+ attP2

• yw; UAS-Nb127D01-GFP, w+ attP40 yw; UAS-BiP-NbVHH05-HA, w+ attP2

• yw; UAS-BiP-NbVHH05-HA, w+ attP40 yw; UAS-BiP-Nb127D01-HA, w+ attP2

• yw; UAS-BiP-Nb127D01-HA, w+ attP40

• yw; UAS-BiP-NbVHH05-GFP, w+ attP2

• yw; UAS-BiP-NbVHH05-GFP, w+ attP40 yw; UAS-BiP-Nb127D01-GFP, w+ attP2

• yw; UAS-BiP-Nb127D01-GFP, w+ attP40 yw; UAS-BiP-VHH05-Upd2-127D01, w+ attP40

• yw; UAS-VHH05-REPTOR-127D01, w+ attP40

• yw; UAS-VHH05-REPTOR-BP-C-127D01, w+ attP40

• w; H2Av-3xVHH05/TM3, Sb w; H2Av-3x127D01/TM3, Sb

Transgenic flies were generated by phiC31 integration of attB-containing plasmids into either attP40 or attP2 landing sites. Briefly, plasmid DNA was purified on QIAquick columns and eluted in injection buffer (100 μM NaPO₄, 5 mM KCl) at a concentration of 200–400 ng/μl. Plasmid DNA was injected into ∼100 fertilized embryos (y,v nos-phiC31int; attP40 or y,w nos-phiC31int; attP2) through microinjection handle (NARISHIGE, Japan) with pressure control (FemtoJet, Eppendorf). The progeny was outcrossed to screen for transgenic founder progeny and the UAS insertions were isolated by screening for white+ eye color.

To generate KI flies, donor and sgRNA plasmids were mixed together and were injected into ∼100 fertilized embryos (y,w; nos-Cas9/CyO). Transformed lines were isolated using a DsRed marker. To remove the DsRed cassette, transformed lines were crossed to a line expressing PBac transposase (BL #8285). Resulting lines were sequenced to confirm the insertion of 3x127D01-tag or 3xVHH05-tag to the C-terminal of the H2Av or Dilp2 gene.

Genomic DNA was extracted from the DsRed2-positive G1 adult animals by standard sodium dodecyl sulfate (SDS) lysis-phenol buffer after incubation with proteinase K (Roche, 3115879001), followed by RNase A (Thermo Fisher Scientific, EN0531) treatment and purification. The 5’- and 3’-end junction fragments at the integration event were cloned separately and sequenced. PCR conditions included 2 min of denaturation at 95°C; 30 cycles of 1 min at 95°C, 30 s at 55°C and 1 min at 72°C; followed by a final extension at 72°C for 10 min by using Takara Taq polymerase (Clontech, #TAKR001). To confirm the removal of the DsRed cassette, the genomic DNA was extracted by the same method and PCR conditions included 2 min of denaturation at 95°C; 30 cycles of 1 min at 95°C, 30 s at 55°C and 30 s at 72°C; followed by a final extension at 72°C for 10 min. PCR products were subcloned into the pJET-1.2 vector (Fermentas, #K1231) and sequenced. Primer sequences are listed in the Supplementary file 1.

For rapamycin treatment, rapamycin (LC Laboratories, R-5000) was dissolved in DMSO and mixed into the media when preparing food vials. The rapamycin dose was 50 µM.

Nanobody expression vectors with C-terminal ALFA-tag or HA-tag for bacterial expression and purification were cloned into pET-26b-Nb-GGA or pET-26b by Golden-Gate assembly or Gibson cloning, respectively. After transformation with BL21 (DE3) competent cells (NEB, C25271), overnight cultures were inoculated in LK media and incubated at 37°C until the OD600 approached ~0.6. Then, 0.1 mM IPTG was added and cultures were placed at 15°C for overnight. After centrifugation, the cell pellet was collected and resuspended in B-PER II Bacterial Protein Extraction Reagent (Thermo Scientific, 78260). After 30 min incubation with rotation, an additional 19× volume of TBS was added and further incubated with rotation. Cleared supernatants were collected after centrifugation at 20,000g and 4 °C for 15 min and used for His-tag purification. Dialyzed His-tag purified nanobody fractions were diluted at 0.2 mg/ml concentration, which were directly used for immunostainings and western blots. To generate fluorescent-labeled nanobodies, Mix-n-Stain CF 555 Antibody Labeling Kit and Mix-n-Stain CF 647 Antibody Labeling Kit (Sigma-Aldrich, MX555S100 and MX647S100) were used according to the manufacturer’s protocol.

Nanobodies equipped with an LPETGG motif and a His-tag at their C-terminus were functionalized with triglycine containing probes using Sortase 5 M as previously described (Cheloha et al., 2019). Briefly, LPETGG motif and His-tag at the C-terminus of nanobodies were replaced with fluorophore or biotin contained in triglycine probes by sortagging reaction. Ni-NTA resin (EMD Millipore, 70691–3) was used to remove unreacted His-tagged nanobodies and His-tagged Sortase 5 M enzyme, and the flow-through (sortase-based labeled nanobodies) was collected. The flow-through was applied to PD-10 column (GE Healthcare, GE17-0851-01) to remove excess triglycine probes.

Drosophila fat body and midguts from adult females were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) at room temperature for 1 hr, incubated for 1 hr in blocking buffer (5% normal donkey serum, 0.3% Triton X-100, 0.1% bovine serum albumin [BSA] in PBS), and stained with primary antibodies overnight at 4°C in PBST (0.3% Triton X-100, 0.1% BSA in PBS). S2R+ cells were fixed in 4% paraformaldehyde in PBS at room temperature for 30 min, incubated for 1 hr in blocking buffer, and stained with primary antibodies for 2–3 hr at room temperature in PBST. The primary antibodies and their dilutions used are: NbVHH05-ALFA (1:500), NbVHH05-HA (1:500), NbVHH05-hIgG (1:20, S2 cell culture media), NbVHH05-555 (1:500), Nb127D01-ALFA (1:500), Nb127D01-HA (1:500), Nb127D01-hIgG (1:20, S2 cell culture media), Nb127D01-647 (1:500), mouse anti-GFP (Invitrogen, A11120; 1:300), rat anti-HA (Sigma-Aldrich, 3F10; 1:1000), rabbit anti-Dilp2 (0.5 µg/ml) (Park et al., 2014 ). After primary antibody incubation, the fat body and midguts or S2R+ cells were washed three times with PBST, stained with 4′,6-diamidino-2-phenylindole (DAPI) (1:2000 dilution) and Alexa Fluor-conjugated donkey-anti-mouse, donkey-anti-rabbit and mouse-anti-hIgG secondary antibodies (Molecular Probes, 1:1000), or NbALFA-Atto647 (NanoTag Biotechnologies, N1502-At647N-L; 1:500) Goat Anti-Alpaca IgG-647 (Jackson ImmunoResearch, 128-605-230; 1:500) in PBST at 22°C for 2 hr, washed three times with PBST, and mounted in Vectashield medium.

All images of the posterior midgut or S2R+ cells that are presented in this study are confocal images captured with a Nikon Ti2 Spinning Disk confocal microscope. Z-stacks of 5–20 images covering one layer of the epithelium from the apical to the basal side were obtained, adjusted, and assembled using NIH Fiji (ImageJ), and shown as a maximum projection.

Cultured cells were harvested 3 days after transfection. For fly, six to eight larval fat bodies or three female midguts per group were dissected in PBS, placed in 50 μl lysis buffer (Pierce, #87788) with 2× protease and phosphatase inhibitor cocktail (Pierce, #78440) and 2 mM trypsin inhibitor benzamidine (Sigma-Aldrich, #434760), and homogenized using Kimble Kontes pellet pestles (Millipore, Z359947). Protein lysates were incubated in 2xSDS sample buffer (Thermo Scientific, #39001) containing 5% 2-mercaptoethanol at 100°C for 10 min, ran on a 4–20% polyacrylamide gel (Bio-Rad, #4561096), and transferred to an Immobilon-P polyvinylidene fluoride (PVDF) membrane (Millipore, IPVH00010). The membrane was blocked by 5% BSA or 5% skim milk in 1× Tris-buffered saline (TBS) containing 0.1% Tween-20 (TBST) at room temperature for 30 min. The following primary antibodies were used: anti-tubulin (Sigma, T5168, 1:10,000), rabbit anti-GFP (Molecular Probes, A-6455; 1:10,000) and anti-FLAG M1 (Sigma, F3040, 1:5000), rat anti-HA (Sigma-Aldrich, 3F10; 1:10,000), NbVHH05 (0.2 mg/ml, 1:5000, or 1:100~1:100,000 used in concentration gradient test), Nb127D01 (0.2 mg/ml, 1:5000, or 1:100~1:100,000 used in concentration gradient test), NbVHH05-hIgG (1:100, S2 cell culture media), Nb127D01-hIgG (1:100, S2 cell culture media) in blocking solution. After washing with TBST, signals were detected with enhanced chemiluminescence (ECL) reagents (Amersham, RPN2209; Pierce, #34095) or fluorescent secondary antibodies information. Western blot images were acquired by Bio-Rad ChemiDoc MP or X-ray film exposure.

For immunoprecipitation using ALFA-tagged nanobodies (NbVHH05-ALFA and Nb127D01-ALFA), His-tag purified nanobodies from bacteria were incubated with ALFA Selector ST resin (Nanotag Biotechnologies, N1511) at room temperature for 1 hr. The resin was washed with Pierce IP lysis buffer (Thermo Scientific, 87787) (3×) and incubated with S2 cell culture media containing secreted GFP proteins with 3xVHH05-tag and 3x127D01 tag at 4°C for 1 hr. After washing in IP lysis buffer (4×), proteins were eluted in 2× sample buffer.

For immunoprecipitation using hIgG-formatted nanobody (Nb127D01-hIgG), Protein A magnetic beads (Bio-Rad, 1614013) were incubated with S2 cell culture media containing Nb127D01-hIgG at room temperature for 1 hr. After washing in IP lysis buffer (3×), beads were incubated with the conditioned media containing secreted GFP proteins 3x127D01 tag at 4°C for 1 hr. After washing in IP lysis buffer (4×), proteins were eluted in 2× sample buffer.

GFP fused at its C-terminus with a peptide corresponding to the extracellular portion of human CXCR2 (full-length, Figure 1—figure supplement 1), produced through sortagging, was immobilized on Nunc 96-well Maxisorp flat bottom plates (100 ng/well). After immobilization, wells were blocked using a solution of BSA in PBS (5% w/v). Nb127D01 conjugated with biotin (20 nM) was mixed with peptides corresponding to full-length CXCR2 extracellular domain or two fragments at varying concentrations. These solutions were then added to plates with immobilized GFP-CXCR2 and incubated for 1 hr. These solutions were discarded from the plates, the plates were washed with PBS containing 0.05% Tween-20, and the amount of Nb127D01-biotin bound to the plate was quantified through the addition of streptavidin-HRP (Thermo Fisher, N100), washing, and the addition of tetramethylbenzidine-containing solution (Thermo Fisher, N301).

Whole-body trehalose levels were measured from five to six groups (each group has four female flies). Fly samples were homogenized with 300 µl TBST buffer (5 mM Tris-HCl [pH 6.6], 137 mM NaCl, 2.7 mM KCl, 0.1% TritonX-100), heated at 75°C for 10 min, and centrifuged at 3000 g for 1 min. Ten µl of supernatant was added to 100 µl glucose assay reagent (Megazyme; K-GLUC) with or without trehalose (1:500; Megazyme; E-TREH) at 37°C for 30 min. The absorbance at 340 nm was measured on a Molecular Devices SpectraMax Paradigm plate reader. The trehalose concentration in the sample was determined by subtracting the glucose concentration from the total sugar concentration.

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

We thank the assistance provided by the Microscopy Resources on the North Quad (MicRoN) core at Harvard Medical School, Christians Villalta for help with the generation of transgenic flies and Hong-Wen Tang for help with the Western blots. We thank Seung K Kim for providing the anti-Dilp2 antibody. This work was supported by NIH NIGMS P41 GM132087. AK is supported by Postdoctoral Fellowship Program (Nurturing Next-generation Researchers) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2021R1A6A3A14039622). JSSL is supported by a Croucher fellowship for Postdoctoral Research from the Croucher Foundation. NP is an investigator of Howard Hughes Medical Institute.

© 2022, Xu et al.

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.