We previously showed that severely altering codon content impacts protein expression from RAS family genes or from a GFP reporter in Drosophila melanogaster and humans (Ali et al., 2017; Lampson et al., 2013; Peterson et al., 2020; Sawyer et al., 2020). This prompted us to systematically determine the impact of codon usage on protein expression. To do so, we again used phiC31 integrase for stable site-specific insertion of a single copy of transgenic codon-modified GFP reporters (Supplementary file 1) into the attP40 locus on chromosome 2. This locus is well-established to express transgenes at robust and reproducible levels (Markstein et al., 2008). As we have done previously (Sawyer et al., 2020), we placed all of our transgenic reporters in the same vector backbone, which uses a ubiquitin (ubi-) promoter sequence for robust gene expression (Methods). In this way, we control for the effects of chromatin environment on gene expression and ensure that any differences in protein production are due to differences in coding sequence.

We took two approaches to introduce rare codons into our GFP reporters. In the first approach, we used a random number generator to select positions in the GFP coding sequence to engineer rare codons. This approach keeps the majority of rare codons dispersed throughout the GFP sequence (Figure 1A). We generated 10 reporters using this dispersed codon strategy, and such reporters are designated with a ‘D.’ Given the potential importance of contiguous stretches of rare codons (Chu et al., 2014; Hayes et al., 2002; Kramer and Farabaugh, 2007; Spanjaard and van Duin, 1988), we also employed a second approach in which all rare codons were clustered at either the 5’ or 3’ end of the coding sequence. We generated six reporters using this clustered codon strategy, and all reporters generated this way are designated ‘C5’ or ‘C3.’ In defining rare codons, we referred to the Kazusa codon database (Nakamura et al., 2000). We identified the most used synonymous codon in the Drosophila melanogaster genome as the ‘common’ codon, the least used codon as the ‘rare’ codon, and any additional synonymous codons between the rare and common codon as ‘middle’ codons. We designed reporters to contain a specific percentage of rare codons, leaving the remaining portion of the coding sequence split between common and middle codons (Figure 1A). These reporters were all designed using an identical eGFP amino acid sequence to rule out any effects of amino acid bias on protein production levels (Weber et al., 2020). All reporters are designated with the fluorophore (e.g. GFP) followed by the percentage of rare codons, followed by a ‘D’ or ‘C’ designation (e.g. GFP50D, GFP70C3’).

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As a convenient method to screen how codon substitutions in GFP impact protein expression across the entire animal, we imaged wandering third instar larvae (WL3) for each transgenic reporter on a fluorescent dissection microscope (Methods). These animals contain fully formed organ systems and are translucent to facilitate imaging. We used the GFP0D reporter with no rare codons as a baseline for comparison. Upon examining animals for each reporter, we noticed an apparent ‘all or none’ fluorescence pattern (Figure 1B–Q). To quantitate our observations seen by fluorescent microscope, we measured protein production by western blot (Figure 1R, Figure 1—figure supplement 1). For reporters with dispersed rare codons, 2/2 reporters with up to 50% rare codons display ubiquitous fluorescence throughout the animal, similar to GFP0D. These two reporters (GFP30D and GFP50D) produce animal-wide GFP protein at levels greater than or equal to 50% of GFP0D (Figure 1B–D and R, Figure 1—figure supplement 1A). In contrast, 7/7 reporters with greater than 50% dispersed rare codons display no visible fluorescence in the animal and produce protein at a level less than 0.3% of GFP0D (Figure 1B, E–K and R, Figure 1—figure supplement 1B,C). Similarly, 6/6 reporters with 50% or more clustered rare codons display no fluorescence (Figure 1L–Q). Two of these six clustered codon reporters, GFP50C5’ and GFP60C3’, produce barely detectable protein levels by western blot, between 7–9% that of GFP0D, whereas the other four reporters yield less protein than 1% of GFP0D (Figure 1R, Figure 1—figure supplement 1C,D). To assess whether mRNA abundance correlates with protein abundance for each reporter, we performed quantitative RT-PCR in each transgenic GFP line and normalized the mRNA abundance to that of GFP0D (Figure 1—figure supplement 1S). Overall, these values show a consistent trend between mRNA and protein abundance for each reporter (Figure 1R vs. S), suggesting that inclusion of rare codons impacts both protein and RNA abundance for these reporters.

While it is not surprising that there would be a rare codon threshold beyond which proteins are no longer produced, what is surprising is the magnitude of the differences in protein production (from robust to barely/not detectable) over a narrow range of rare codon usage. A 50% increase in rare codon usage between GFP0D and GFP50D causes a 50% reduction in protein levels. Yet an additional 10% increase in rare codon usage between GFP50D and GFP60Dv1 decreases detectable protein by 99.5% (Figure 1R, Figure 1—figure supplement 1A).

To determine if the steep decline in protein levels between 50 and 60% dispersed rare codons is robust and reproducible, we independently designed two more 60% dispersed rare codon reporters. These reporters, GFP60Dv2 and GFP60Dv3, contain rare codons that differ in sequence/position from GFP60Dv1. All three GFP60D reporters show the same fluorescence pattern (no detectable GFP), and when protein levels are compared to GFP50D by western blot we again observe a decrease in protein levels of at least 99.5%, with a 99.85 decrease on average across all three GFP60D reporters (Figure 1E–G and R, Figure 1—figure supplement 1B). Overall, our results indicate there is a narrowly defined window of rare codon content in which protein production drops from robust to barely detectable/none.

We next put our observed cliff-like decline in protein levels from reporters in context with endogenous Drosophila melanogaster gene sequences. To look at the codon usage of endogenous genes, we turned to the well-established Codon Adaptation Index (CAI) as a metric for measuring codon usage optimality (Sharp and Li, 1986). CAI calculates the degree of optimal codon usage in a transcript based on the usage frequency of that codon in a reference set of genes. Higher CAI scores indicate more common codon usage, where a score of 1 indicates a transcript only uses the most common codons for each amino acid (Nakamura et al., 2000). We plotted the CAI values of each individual transcript in the Drosophila melanogaster genome (Figure 1T, Methods). We similarly computed the CAI values for each of our codon-modified GFP reporters (Figure 1T).

Comparing CAI values between reporters and endogenous genes reveals a striking cliff-like protein expression limit imparted by rare codons. This limit is reflected in the all-or-none expression pattern of our reporters. The lowest CAI of any reporter (GFP50D, Figure 1R, Figure 1—figure supplement 1) with detectable fluorescence resides near the limit of CAI for endogenously expressed genes (only above the CAI of 2% of all expressed genes- Figure 1T, see left boundary of gray rectangle). Below the CAI value of GFP50D, none (0/13) of our reporters are expressed at the level of detectable fluorescence, and only two of these reporters produce protein detectable by western blot at low levels (Figure 1R, Figure 1—figure supplement 1). We note that we find no correlation between mRNA or protein length and CAI in Drosophila (Figure 1—figure supplement 2), in contrast to a previous study comparing frequency of optimal codons to protein length (Duret and Mouchiroud, 1999). Our findings suggest that for endogenous genes in Drosophila, a limit on robust rare codon-derived protein expression is in the range of 50–60% rare codon usage or between CAI scores of 0.61 and 0.57. This limit is experimentally validated by the failure of 13/13 transgenic reporters below this range to produce appreciable levels of protein (Figure 1R, Figure 1—figure supplement 1), and is reflected by the rarity of endogenous genes at or below this range (Figure 1T).

While overall we observe a cliff-like threshold in reporter GFP levels (Figure 1R, Figure 1—figure supplement 1), none of the clustered rare codon reporters reside within our determined CAI-dependent limit to be detected by fluorescence. Given this, we generated another clustered codon reporter (GFP54C3’). In designing GFP54C3’, we clustered all of the rare codons in the 3’ end of the coding sequence and used only the most common or most rare codons for each amino acid. Compared to GFP50D, this design increased rare codon content while maintaining a higher overall CAI score (0.64, Figure 2A). GFP54C3’ falls near the limit of CAI for endogenously expressed Drosophila genes, ranking higher than just 5% of endogenous transcripts. Unlike the all-or-none expression pattern of the reporters shown in Figure 1, GFP54C3’ shows no signal in most of the animal but has a robust GFP fluorescence in two distinct areas of the larva (Figure 2C). This suggests that distinct tissues respond differently to rare codons.

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Given the unique tissue-specific pattern with this reporter, we generated additional reporters to further understand the sequence parameters driving this expression. To assess whether the tissue-specific expression of GFP54C3’ is due to an unknown sequence motif, we generated an entirely different reporter with similar sequence parameters. To do so, we generated a hybrid mCherry/GFP reporter. Both mCherry and GFP are similar in size (708 and 717 nucleotides, respectively). Since the rare codons in GFP54C3’ are clustered towards the 3’ end, we designed mGFP100Dv1, where we redesigned GFP100D by creating a fusion protein linked to a 5’ mCherry sequence (Methods). This mCherry is naturally enriched in common codons, and the fusion to GFP100D creates a reporter with a CAI similar to GFP54C3’ (Figure 2B). mGFP100Dv1 rescues the lack of expression seen with GFP100D in a tissue-specific manner (Figure 2D vs. Figure 1K). Using further altered versions of this hybrid mCherry/GFP reporter (Figure 2—figure supplement 1A, Methods), we ruled out that the tissue-specific rescue of expression is due to an unknown sequence motif in the mCherry or fusion linker (Figure 2—figure supplement 1B–G). We also examined the sequences of all reporters for co-occurring codon pairs known to impact translation fidelity, such as AGG and CGA (Letzring et al., 2010; Spanjaard and van Duin, 1988), and find no predictive pattern in their occurrence between our tissue-specific and non-tissue-specific reporters (Supplementary file 2).

Having established that tissue specificity can be achieved using multiple different coding sequences, we next determined which tissues express rare codon-enriched GFP54C3’ and mGFP100Dv1. In larvae, we dissected out the two bright tissues from mGFP100DV1. In doing so, we noticed that male larvae always have two bright tissues, whereas female larvae always have one. Our dissections revealed this difference to be because mGFP100DV1 expresses brightly in the larval testis (Figure 2E) but is not detectable in larval ovaries (Figure 2F). The other mGFP100DV1 bright tissue, in common to both female and male mGFP100DV1 larvae, is the brain (Figure 2G). Together, our results reveal that, within a narrow range of 50–60% rare codons, a select few tissues are capable of robustly producing protein from rare codon-enriched transcripts (Figure 2H).

We next turned to adult animals, to assess if the distinct testis and brain expression carries forward to this stage. We used a combination of fluorescence microscopy of isolated tissues (Figure 3A–L’) and quantitative western blotting (Figure 3M, Methods). This analysis again revealed that the testis and brain express mGFP100Dv1 with the testis having the strongest relative protein expression (Figure 3E, F and M). Further, we find that other tissues, such as the ovary and male accessory glands, do not express this reporter at a detectable level (Figure 3G, H and M). Conversely, common codon-enriched GFP0D is readily detectible, albeit at different cell type-specific levels in all adult tissues examined (Figure 3A–D and M, Figure 3—figure supplement 1). This suggests that lack of mGFP100Dv1 expression is not due to strong tissue-level differences in promoter strength between tissues.

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To quantify the impact of rare codons on protein production in each tissue, we compared protein levels of mGFP100Dv1 to GFP0D from three replicate western blots (Figure 3M, Figure 3—figure supplement 1). There is no statistically significant difference in protein levels between GFP0D and mGFP100Dv1 for both testis and brain in adult males, consistent with these tissues having a distinctly higher tolerance for rare codons (Figure 3M). In contrast, both the ovary and accessory gland produce mGFP100Dv1 protein at levels less than or equal to 2% that of GFP0D protein (Figure 3M), consistent with a lower rare codon tolerance in these (and most other) Drosophila tissues (Figure 2H). While we do not observe differences in the ability of the brain to express rare codon-enriched reporters between sexes (Figure 3—figure supplement 2), the testis but not the ovary robustly expresses protein derived from rare codon-enriched reporters (Figure 3M). One possible explanation for the tissue-specific differences in protein expression could be differences in the strength of the ubiquitin promoter in each tissue. However, our fluorescence-based approach suggested this is not the case (Figure 3A–D,). We further assessed this by examining the levels of GFP0D (all common codons) by western blot in each tissue. Interestingly, the ovary expresses GFP0D at similar levels to the testis, yet rare codon-enriched mGFP100Dv1 is expressed 100-fold higher in the testis than in the ovary (Figure 3—figure supplement 1B). This confirms that promoter strength is not a confounding variable. A second confounding variable could be chromatin context. To assess if testis and brain specificity of an mGFP100D reporter still occurs when integrated at a different locus, we integrated a reporter with similar codon content (mGFP100Dv8) into the attP2 locus on chromosome 3, and again observed the same testis and brain specificity of this rare codon-enriched reporter (Figure 3—figure supplement 3). Collectively, these results highlight striking differences between Drosophila tissues, most notably between the male and female gonad, with regards to rare codon-derived protein expression.

Our findings on rare codon-derived protein expression at a whole tissue level, which are most pronounced in the testis, prompted us to further examine which specific cell types in the testis can robustly express rare codon-enriched reporters. Multiple cell types comprise the Drosophila testis, including both somatic and germline cell lineages (Fairchild et al., 2017; Herrera and Bach, 2019; Lim et al., 2015; Yamashita et al., 2005). To determine which specific cell types express protein from rare codon-enriched reporters, we used cell type-specific antibodies and assessed co-localization with rare codon-enriched GFP54C3’. Male germ cells (Vasa positive) and the somatic hub cells (FasIII positive), which comprise the male germline stem cell niche, robustly express GFP54C3’ (Figure 3I–J’), whereas somatic cyst cells that encapsulate the germline cells (Eya positive) do not (Figure 3K and K’). Importantly, somatic cyst cells robustly express common codon-enriched GFP0D (Figure 3L and L’), indicating that the ubiquitin promoter is active in this cell type. Thus, both the male germ cells and the specialized somatic cells of the male germline stem cell niche express protein derived from a rare codon-enriched reporter, while the larger population of somatic cyst cells lack this capability. Together, we find a striking difference between male and female gonads in the expression of protein from rare codon-enriched genes.

Our observed tissue-specific protein expression of rare codon-enriched reporters in the testis and brain could be primarily driven at the protein level, but could also be regulated at the level of mRNA (reviewed in Radhakrishnan and Green, 2016). Rare codons can decrease levels of transcription (Fu et al., 2018; Yang et al., 2021; Zhao et al., 2021; Zhou et al., 2016) or can negatively impact mRNA stability through a growing number of characterized mechanisms and in numerous model systems (Bazzini et al., 2016; Burow et al., 2018; Buschauer et al., 2020; Presnyak et al., 2015; Radhakrishnan et al., 2016; Wu et al., 2019; Zhao et al., 2017). Alternatively, translational repression mechanisms can act independently of mRNA stability, further complicating the relationship between codon optimality and mRNA levels (Freimer et al., 2018).

We next assessed whether reporter protein abundance reflects mRNA levels in different tissues. The highest mRNA level for mGFP100Dv1 is found in the testis (Figure 3N). Further, mRNA levels of mGFP100Dv1 and GFP0D are nearly identical in the testis. This result matches our finding at the protein level in the testis (Figure 3M vs. N). mGFP100Dv1 mRNA is also detected in several other tissues, but at a reduced level relative to GFP0D. Specifically, we detect mGFP100Dv1 mRNA in the ovary (50% of GFP0D levels), brain (30% of GFP0D), and accessory gland (10% of GFP0D, Figure 3N). Among the non-testis tissues examined, only the brain appears able to convert this mRNA into abundant protein. This result is most apparent when plotting the relative translation efficiency (TE) of mGFP100Dv1 in each tissue (Figure 3O). In contrast, the ovary is capable of accumulating mGFP100Dv1 mRNA (Figure 3N), but translation is impaired (Figure 3M and O). Taken together, these results indicate that both mRNA abundance (from the combined effects of transcription/mRNA decay) and translation efficiency of rare codon-enriched reporters are impacted by tissue context. Further, these mRNA results support our finding that, relative to all other tissues examined, gene expression in the testis is less impacted by codon bias.

Mechanistically, each tissue could process the GFP reporter pre-mRNA differently, accounting for different protein abundances. To explore this possibility, we assayed for the presence of four possible alternatively spliced mGFP100Dv1 mRNAs that could be produced from our transgene in each tissue (Figure 3—figure supplement 4A,B). We observe no obvious difference in mRNA processing (Figure 3—figure supplement 4C,D, Supplementary file 3). Hence, mRNA processing does not appear to substantially contribute to tissue-specific differences for our codon-altered reporters. We next sought to put our findings with synthetic reporter mRNA levels and codon content in context with endogenous genes. Many established bioinformatic metrics related to codon usage such as the species-specific tRNA Adaptation Index (stAI), which utilizes tRNA gene copy numbers to predict translation efficiency, reflect variables that do not change within an organism’s tissues (Sabi and Tuller, 2014). And indeed, we do not observe differences in stAI between Drosophila tissues (Figure 4—figure supplement 1). Therefore, we developed a modification to the CAI to create a new codon usage metric that reflects codon usage among highly expressed mRNAs that are enriched in a specific tissue. We term this metric tissue-apparent CAI (taCAI). In developing taCAI, we first defined tissue-specific gene sets from publicly available tissue-specific Drosophila RNAseq datasets (Methods) for each tissue using cutoffs based on relative expression levels (Methods). From these tissue-specific gene sets, we then took the 300 most enriched genes for each of 12 adult tissues and obtained the usage frequency for each codon within that set of over-represented genes. The codon usage frequencies for each coding sequence in the genome were then compared against usage frequencies within the tissue-specific gene set to obtain a spread of taCAI values visualized as violin plots. Low taCAI values indicate that mRNAs expressed in a tissue are enriched for rare codons, while high taCAI values indicate that mRNAs expressed in a tissue are enriched for common codons. We calculated taCAI for individual adult tissues in Drosophila melanogaster for which RNAseq data exists in FlyAtlas2 (Krause et al., 2022; Methods).

When visualizing the taCAI distributions for each tissue as a violin plot (Figure 4A), we notice a clear tissue-specific trend. The testis and accessory gland, both parts of the male reproductive system, are highly enriched for mRNAs with abundant rare codon usage relative to all other tissues and to the transcriptome as a whole. We note that the accessory gland does not accumulate rare codon-enriched reporters, and the brain does not have a particularly high taCAI (see Discussion). In the testis, our computational findings with taCAI match our experimental results using rare codon-enriched GFP reporters. Taken together, our results show that among the tissues examined, the Drosophila testis accumulates by far the highest mRNA and protein levels for a rare codon-enriched reporter. This finding is consistent with high levels of mRNA for endogenous testis-expressed, rare codon-enriched genes.

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We next examined human genes for tissue-specific differences in codon usage. We obtained transcriptomic data from the Human Protein Atlas (HPA; Uhlén et al., 2015) project and computed taCAI for each tissue. After correcting for the larger number of distinct human tissues with RNAseq data (37 vs. 12, see Methods and Figure 4—figure supplement 2), the testis again emerged as a unique rare codon-enriched outlier tissue, along with the pancreas (Figure 4B). While available proteomic data for human tissues does not have the same depth as RNAseq data, we took an available human tissue specific proteomic dataset (Jiang et al., 2020) and analyzed the CAI of each tissue. In support of our taCAI analysis, again the testis was the top-ranked tissue (meaning it has a high number of proteins derived from mRNAs with abundant rare codons Figure 4—figure supplement 3). These data argue for a conserved and distinctive regulation of mRNAs from rare codon-enriched genes in the testis.

The abundance of rare codon-enriched mRNAs in the testis suggests that rare codons could limit expression and/or impact function of an mRNA/protein to this tissue. Given this, we next searched for an example where rare codons in a testis-specific gene are critical to the animal. The ‘out-of-testis hypothesis’ (Assis and Bachtrog, 2013; Kaessmann, 2010) posits that the testis provides a permissive environment for evolution of new genes. This is based on observations in numerous organisms that young genes tend to be testis-specific and gradually gain broader tissue function as they age (Betran, 2002; Kondo et al., 2017; Marques et al., 2005). The restriction of young genes to the testis has been largely considered a passive consequence of permissive chromatin states during meiosis in the germ cells (Soumillon et al., 2013). However, the reduced impact of rare codons on mRNA and protein expression in the testis may provide an alternative mechanism for restricting the expression/function of young genes to this tissue.

To examine the codon content of evolutionarily young genes, we analyzed data on young genes arising from duplication events (Zhang et al., 2010). Following a duplication event, there is a parent gene copy that often retains its ancestral function and a child gene copy, which may evolve a new function. We analyzed the codon content of evolutionarily young parent and child gene pairs arising from retrotransposition-mediated duplications identified by Zhang et al., 2010. We analyzed the CAI of 96 such parent-child gene pairs. These data reveal that child genes arising from retrotransposition tend to have lower CAI values than their parent genes (Figure 5A). We found that in roughly half (44/96) of these retrotransposition events, child genes have maximum expression in the testis among all tissues analyzed (N=12 tissues), while their parent genes were highest expressed in a tissue other than testis (Supplementary file 4). These findings imply that rare codons may restrict the expression/function of evolutionarily young genes to the testis.

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In several cases, the difference between parent and child can span nearly the entire range of CAI for expressed genes (with the parent at the common end and child at the rare end, Figure 5A see Figure 1T for the genomic range). From these examples, we chose a candidate gene. The gene encoding the testis-specific ribosomal subunit RpL10Aa has the second largest difference in CAI between parent and child gene of any young retrotransposition-mediated duplication in Drosophila. The ancestral copy of RpL10Aa, RpL10A, is a highly conserved member of the 60 s ribosomal subunit. Within the subgenus Sophophora, approximately 10 million years ago a gene duplication event, likely mediated by retrotransposition (Marygold et al., 2007; Zhang et al., 2010), gave rise to two isoforms in the melanogaster subgroup (Figure 5B). This duplication produced a parent gene copy, RpL10Ab, and a child copy RpL10Aa. We chose to study RpL10Aa because of its young evolutionary age, and because of the striking differences in codon usage and tissue expression pattern between RpL10Aa and the parent gene RpL10Ab. RpL10Aa is highly enriched in rare codons (CAI 0.66) placing it in the rarest 8% of genes organism-wide, is testis-specific in expression pattern (Figure 5C), and is very highly expressed in the testis, ranking in the top 2.5% of all genes expressed in the testis. In contrast, RpL10Ab has very few rare codons (CAI 0.82), ranking it above 95% of genes organism-wide, and is expressed relatively uniformly throughout the body (Figure 5C). That such a drastic reduction in codon bias coupled with high testis-specific gene expression occurred over a short evolutionary period suggests that rare codons may have been positively selected for in RpL10Aa.

To examine the role of rare codon enrichment in a young testis gene, we analyzed protein levels for both an endogenous RpL10Aa sequence (RpL10Aa Endo) and a codon optimized RpL10Aa sequence with all common codons (RpL10Aa Com). We did this by placing both coding sequences behind the same ubiquitin promoter used for our organism-wide GFP reporter screen (Figure 5D). To specifically measure RpL10Aa protein from transgenes and not from the endogenous gene locus, we included an N-terminal 3x-FLAG tag in both the endogenous and codon optimized RpL10Aa sequences. By western blot, we observe that our ectopically expressed transgenes could be detected in not only the testis, but also the ovary, gut, and brain (Figure 5—figure supplement 1). This suggests that the endogenous codon content of RpL10Aa alone is not sufficient to restrict gene expression to the testis when driven by a strong ectopic promoter. In comparing RpL10Aa Endo and Com protein levels, we find that RpL10Aa Com protein is 2-fold higher than RpL10Aa Endo protein in both testis and in ovaries (Figure 5E, Figure 5—figure supplement 2). These measurements establish that codon optimization increases protein abundance from an ectopic RpL10Aa gene, both in the testis (where RpL10Aa is highly expressed) and in the ovary (where RpL10Aa is not normally expressed).

Our previous study of codon-dependent effects of Ras signaling in Drosophila identified profound phenotypic differences of a 2-fold, codon-dependent increase in protein expression (Sawyer et al., 2020). Indeed, while generating stable lines expressing ectopic RpL10Aa, we noticed that RpL10Aa Com animals are much less fertile than RpL10Aa Endo animals. We hypothesized that female fertility but not male fertility is impacted by codon optimization of the RpL10Aa coding sequence, given the tissue specificity of RpL10Aa mRNA expression from its endogenous locus (Figure 5C). To evaluate male and female fertility for RpL10Aa Com and RpL10Aa Endo animals, we performed reciprocal single fly crosses between our transgenic flies and wildtype flies. Throughout the course of our fertility assay, we observed high female lethality in RpL10Aa Com animals (11/30 females, 1/10 males) and low lethality in RpL10Aa Endo animals (2/10 females, 0/10 males). Only crosses where both parents survived the duration of the study were analyzed with respect to fertility. We observe no difference in male fertility between endogenous and codon optimized RpL10Aa animals when crossed to wildtype females (Figure 5F). For the reciprocal cross (wildtype males crossed to transgenic females), we instead observe drastic differences in female fertility between reporters. RpL10Aa Com females produced just 6 viable offspring on average compared with RpL10Aa Endo females, which produced on average 452 viable offspring (Figure 5F). To more closely examine the cause of female infertility in RpL10Aa Com females, we examined adult ovary morphology. While early (pre-stage 8) ovarioles appear normal, stage 8–10 ovarioles of RpL10Aa Com animals contain numerous pyknotic nuclei (Figure 5G–I). Therefore, optimizing codon content of RpL10Aa in an ectopic reporter context specifically disrupts female (not male) fertility, due to disrupted oogenesis (see Discussion).

Our findings suggest that optimizing codons in RpL10Aa increases RpL10Aa protein expression in both testis and ovary, but only the ovary is negatively impacted. This could be partly because (as suggested by RNAseq, Figure 5C), the testis naturally accumulates (and tolerates) a high level of RpL10Aa mRNA, whereas the ovary does not. To assay the relative mRNA abundance of endogenous vs. codon-optimized RpL10Aa in our transgenic animals, we measured the relative amount of endogenous and codon-optimized RpL10Aa mRNA in both testis and ovary. In these experiments, we used primers that simultaneously detect both possible forms of mRNA from endogenous RpL10Aa sequence (both transgenic RpL10Aa Endo mRNA and RpL10Aa mRNA from the endogenous locus). We separately examined RpL10Aa Com mRNA using primers specific to mRNA from this transgene. Whereas the ovary has a lower ratio of endogenous to common RpL10Aa mRNA, the testis has the reverse- a higher level of endogenous to common RpL10Aa mRNA (Figure 5J). We interpret this to reflect the high level of endogenous RpL10Aa already present in the testis. Taken together, our findings illustrate an important role for rare codons in tissue-specific gene expression and imply that rare codons in the normally testis-specific gene RpL10Aa help to preserve fertility.

Redundancy of the genetic code has long been a mystery of the central dogma of molecular biology. Here, we use Drosophila melanogaster to uncover distinct tissue-specific responses to the same genetic code. Our work uncovers fundamental aspects of rare codon biology. We reveal a cliff-like limit on rare codon usage per gene. Near the boundaries of this limit, however, we demonstrate that individual tissues have different tolerances for protein expression from genes enriched in rare codons. Tissues with a high tolerance for rare codons include the brain of both sexes and the testis, but not the ovary. Taking a closer look at the testis, we find the male germ cells and somatic cells of the germline stem cell niche of the testis robustly express protein from rare codon-enriched reporters, while the somatic cyst cells do not. In developing a new metric for tissue-specific codon usage, taCAI, we reveal endogenous genes in the testis of both Drosophila and humans show an abundance of rare codon-enriched mRNAs, suggesting that rare codon-enriched genes play an essential and conserved role in testis biology. In search of a physiologically relevant role for rare codon tolerance in testis biology, we demonstrate that endogenously rare codons may restrict the expression and function of the evolutionarily young gene RpL10Aa to the testis. Codon optimization of an RpL10Aa transgene disrupts female fertility and organism viability. Taken together, our results uncover clear tissue-specific differences in the impact of codon usage bias and partially support a novel role for rare codons in restricting the expression of evolutionarily young genes to the testis.

Here, our sensitive reporter-based approach suggests a strong correlation between CAI and reporter GFP abundance in whole Drosophila (R²=0.82, p<0.0001). We note that previous data from bioinformatic approaches in human tissues are conflicting regarding correlations between CAI and tissue-specific mRNA and protein expression (Plotkin et al., 2004; Sémon et al., 2006). Further, similar bioinformatic studies suggested only weak codon-dependent differences between tissues in Drosophila (Payne et al., 2019). The sensitivity of our reporter and the ability of our expression platform to rapidly test various sequences over a narrow CAI range proved helpful in defining rare codon limits for protein expression, which closely mirror existing mRNA expression data for the testis. Our study reveals a strict limit to rare codon usage on a genomic scale. This limit could reflect the tipping point in rare codon usage before competition between transcription and mRNA decay favors degradation over translation (Buschauer et al., 2020; Radhakrishnan et al., 2016).

We also observe clear differences in the impact of codon usage bias between Drosophila tissues. Importantly, we show how distinct tissues respond differently to the same genetic code. It is well appreciated that tissue-specific chromatin environments play critical roles in transcriptional regulation. Our findings here suggest that tissue-specific tolerances for rare codons play a similar role at the levels of translation and transcription/mRNA decay. There are several likely candidate mechanisms underlying tissue-specific impacts of codon bias, including differences in levels of RNA decay machinery or tRNAs between tissues. Several RNA decay pathways have been linked to rare codon usage and would be of interest to follow up on in our system (Buschauer et al., 2020; Radhakrishnan et al., 2016). Levels of expressed tRNAs have been previously measured in Drosophila at the organismal level (Moriyama and Powell, 1997; White and Tener, 1973), but not at the level of individual tissues. Quantifying tRNA expression is not the same as measuring tRNA activity, however, as tRNAs exhibit rigid folding structure, distinct amino acid carrying status when active, and require post transcriptional modifications. The contribution of tRNA regulation to tissue-specific codon bias regulation can be explored further in the future.

We note that mechanisms, which lead to rare codon mRNA/protein accumulation, may be distinct in different tissues. For example, our taCAI analysis of RNAseq data revealed that the testis but not the brain is enriched for mRNAs with abundant rare codons. Recent work by other groups, however, demonstrates that rare codons have less influence on mRNA stability in central nervous system compared to whole embryo (Burow et al., 2018), supporting our reporter-based finding here that the brain has unique biology pertaining to rare codons. In addition, we find that while the testis is capable of translating GFP0D and mGFP100Dv1 mRNA with relatively equal efficiency, the brain appears to translate mGFP100Dv1 even more efficiently than GFP0D. This suggests that the brain may have specialized translational machinery or tRNA pools better adapted to rare codons than to common ones, whereas the testis is equally adapted to both rare and common codon pools. Further, while the accessory gland did not robustly express our rare codon-enriched reporters, our taCAI analysis suggests this male reproductive tissue is also enriched in mRNAs with abundant rare codons. Now that we have pinpointed interesting differences between the male reproductive tract, brain, and other tissues, these differences can be exploited in future studies to reveal the underlying molecular mechanisms.

Here, we find a conserved enrichment for rare codons in testis-specific genes. Further, we demonstrate a role for rare codons in the evolutionarily young RpL10Aa, a known testis-specific gene. Optimizing the codon sequence of this testis-specific gene causes ectopic high expression in the ovary and results in female infertility. Specifically, the ovary is known to activate a mid-oogenesis apoptotic checkpoint (Pritchett et al., 2009), and our observed phenotypes are consistent with ectopic codon-optimized RpL10Aa disrupting mid-oogenesis. We note that our ubi- promoter is stronger in the soma than the germline (Figure 3—figure supplement 1C), which may suggest a somatic origin of the phenotypes we observe. We speculate that ectopic RpL10Aa protein in the ovary may act as a dominant negative relative to the function of the parent gene RpL10Ab (64% identity between these proteins), which is required for female fertility (Wonglapsuwan et al., 2011). However, an alternative is that the phenotypes we observe are mRNA mediated, rather than protein mediated.

Tissue specificity imparted by rare codons may have functional relevance to the ‘out-of-testis’ hypothesis for new gene evolution (Kaessmann, 2010). This hypothesis proposes that the testis acts as a ‘gene nursery’ to promote evolution and neofunctionalization of young genes through its permissive expression environment and intense selective pressures from both mate and sperm competition. This is based on observations that testes are the fastest evolving tissue and that young genes are often restricted in expression to the testis and gain broader expression patterns as they age.

While the current model is that permissive chromatin states passively allow expression of young genes resulting from retrotransposition events specifically in the testis (Kaessmann, 2010). Here, we suggest that rare codon content may also fulfill this role and could be positively selected for an evolutionarily young genes in Drosophila. Male-biased genes have also been characterized to have rarer codon usage than female-biased genes and non-sex-biased genes in numerous plant species (Darolti et al., 2018; Song et al., 2017; Whittle et al., 2007) and Drosophila (Hambuch and Parsch, 2005), and have rarer codon usage than non-sex-biased genes in zebrafish and stickleback (Yang et al., 2016). Thus, our findings may be widely applicable to sexually reproducing species across phylogenetic kingdoms. Taken together, we highlight codon usage as a critical and under-appreciated aspect of tissue-specific gene regulation that merits greater attention, notably in the fields of evolutionary and developmental biology.

Codon-modified exon sequences for GFP and RpL10Aa were designed according to the codon usage frequencies in the Drosophila melanogaster genome taken from the Kazusa codon usage database (https://www.kazusa.or.jp/codon/). The mCherry sequence used in generating fusion proteins was obtained from NovoPro pADH77. Sequences were subsequently generated through gene synthesis (ThermoFisher Scientific and Invitrogen, Waltham, MA) and cloned into a pBID-Ubi plasmid (modified from Addgene Plasmid #35200) or were directly synthesized into the desired pBID-Ubi plasmid cloning site (Twist Bioscience, South San Francisco, CA). Plasmids were grown in NEB 5-alpha competent E. coli cells (NEB #C2987), transformed according to the manufacturer’s protocol, and purified with a ZymoPure II Plasmid Midipred Kit (Zymo Research). Plasmids were injected into either attP40 (2 L) or attP2 (3 L) flies by Model System Injections (Durham, NC). Full sequences for the synthesized genes are provided in Supplementary file 1. Sequences for the Ubi-p63E promoter and transgenic reporter UTRs are provided in Supplementary file 3.

All flies were raised at 25 °C on standard media unless noted otherwise (Archon Scientific, Durham, NC). The following stock was obtained from the Bloomington Drosophila Resource Center w¹¹¹⁸ (#3605). The following stocks were generated for this study: GFP30D, GFP60Dv1, GFP60Dv2, GFP60Dv3, GFP70D, GFP80D, GFP90D, GFP100D, GFP50C5’, GFP50C3’, GFP60C3’, GFP70C3’, GFP80C3’, GFP90C3’, GFP54C3’, mGFP100Dv1, mGFP100Dv2, mGFP100Dv3, mGFP100Dv4, mGFP100Dv5, mGFP100Dv6, mGFP100Dv7, mGFP100Dv8, RpL10Aa Endo, RpL10Aa Com.

Protein samples were prepared as in Sawyer et al., 2020. Briefly, tissues or whole animals were homogenized in Laemmli buffer (10 tissues in 50 µl or five animals in 100 µl) on ice, then boiled for 5 min. Samples were separated on 12% sodium dodecyl sulfate polyacrylamide gels by electrophoresis (SDS-PAGE) at 200 V. Proteins were transferred onto nitrocellulose membranes using an iBlot 2 Dry Blotting System (Invitrogen, Waltham, MA) set to 20 V, 6 min. Total protein was quantified using Revert700 Total Protein Stain Kit (LI-COR Biosciences, Lincoln, NE) according to the manufacturer’s protocol for single channel imaging. For normalization, we note that total protein stain was superior to antibodies against single housekeeping genes (Tubulin) due to variable expression of these proteins between tissues. The following antibodies were used: rabbit anti-GFP (1:1000, Invitrogen, cat#A11122), anti-FLAG M2 (1:500, Sigma, St. Louis, MO, cat#F1804), IRDye 800CW (1:10,000, LI-COR Biosciences, anti-rabbit or anti-mouse). Signal was detected using a LI-COR Odyssey CLx and analyzed using Image Studio version 5.2 (LI-COR Biosciences). Original source data for all western blots have been provided.

CAI calculations for transgenic reporters and endogenous Drosophila genes were performed using CAIcal (Puigbò et al., 2008). For CAI calculation of endogenous genes, the standalone version of CAIcal was used with input CDS sequences from the Drosophila melanogaster genome version r 6.22, and the Drosophila melanogaster codon usage table from the Kazusa codon usage database (Nakamura et al., 2000).

Tissue-specific RNA sequencing data was obtained for Drosophila from FlyAtlas2 and for humans from the Human Protein Atlas. We defined genes as being tissue-specific in Drosophila if they were highly expressed in the tissue of interest, and not highly expressed in any other tissue. Specifically, genes had to be among the top 40% of genes expressed in the tissue of interest and excluded from the top 25% of genes expressed in any other tissue analyzed. We chose a thresholding, rather than a fold change, approach to determine tissue specificity. This was because some tissues have a much higher raw expression value than others (the median expression value is more than double for one tissue vs. another tissue in some cases), which confounds fold change approaches. We chose to analyze the 300 most enriched tissue-specific genes per tissue because any value higher than 300 prevented us from examining an equal number of genes in each tissue. Applying the same gene selection filter to human tissues revealed that testis genes were most enriched in rare codons among the 37 tissues analyzed (Figure 4—figure supplement 1), however, the 3-fold larger number of tissues in humans compared to flies (37 vs. 12) prompted us to explore additional, more stringent cutoffs of tissue specificity for the human data. Towards this end, we defined genes as being tissue-specific in human if they were highest expressed in the tissue of interest and had a TPM <1 in all other tissues analyzed (Figure 4B). Codon usage frequency was calculated using the Kazusa Countcodon program (https://www.kazusa.or.jp/codon/countcodon.html). We used the resulting tissue-specific codon frequency table to compute taCAI values for each tissue using the following equation:

where $N$ is the number of genes in the genome and ${CAI}_i$ is the codon adaptation index value calculated for gene $i$ using the tissue-specific codon frequency table for tissue $t$. This calculation returns a genome-sized distribution of values, where the codon usage of each gene is evaluated relative to the codon usage of a pre-defined tissue-specific gene set. The distribution of taCAI values for a given tissue can be visualized as a violin plot and these distributions can be compared between tissues. To additionally visualize how the codon usage of each tissue compares to the codon usage of the entire genome, we computed the genomic taCAI distribution using the following equation:

where $N$ is the number of genes in the genome and ${CAI}_i$ is the codon adaptation index value calculated for gene $i$ using the codon frequency table for the entire genome $g$.

Species-specific tRNA Adaptation Index (stAI) values were calculated for the same Drosophila tissue-specific gene sets used for calculating tissue-apparent CAI (taCAI). This was done using the standalone stAIcalc program (Sabi et al., 2010) with input tRNA gene copy numbers from the genomic tRNA database (http://gtrnadb.ucsc.edu/index.html, Drosophila melanogaster Aug. 2014 BDGP Release 6 /dm6). The standalone stAIcalc application allows for a user defined variable hill climb stringency setting. stAI calculations were performed using both the minimum allowed hill climb stringency setting and the maximum allowed hill climb stringency setting.

For tissue-specific measurements of mGFP100Dv1 mRNA, animals heterozygous for both mGFP100Dv1 and GFP0D were aged 3–7 days at 25 °C on standard fly medium supplemented with wet yeast (Archon Scientific, Durham, NC). For tissue-specific measurements of RpL10Aa-Endo mRNA, animals heterozygous for both RpL10Aa-Endo and RpL10Aa-Com were aged 3–7 days at 25 °C on standard fly medium supplemented with wet yeast. For whole larval measurements of GFP reporter mRNA, WL3 larvae heterozygous for the indicated reporter and GFP0D were raised at 25 °C on standard fly medium. Where applicable, dissections were performed in RNase-free phosphate buffered saline and completed in under 2 hr. After dissection, tissues were immediately homogenized in TRIzol reagent (N=10 animals per tissue per replicate, 500 µl TRIzol reagent) and snap frozen in liquid nitrogen before storage at –80 °C. For whole larval samples, five WL3 larvae were homogenized in 500 µl TRIzol reagent. RNA was purified according to the manufacturer’s protocol, using glycogen as a carrier and resuspending in molecular grade water. RNA was then treated with DNase I at room temp for 15 min before terminating the reaction by adding 2.5 mM EDTA and incubating at 65 °C for 10 min then storing at –80 °C. Quantification of RNA was performed on either a NanoDrop spectrophotometer or Qubit 3 fluorometer and samples were diluted to match the concentration of the lowest concentration sample. Equal amounts of RNA for all samples directly compared to one another were simultaneously transcribed into cDNA using iScript cDNA synthesis kit (BIO-RAD, Hercules, CA, cat#170–8891) according to the manufacturer’s protocol within 7 days of the initial dissections to preserve sample quality. No Reverse Transcriptase (NRT) controls were also run simultaneously for each sample to control for genomic DNA contamination. Quantitative Real-Time PCR (qRT-PCR) was run simultaneously on all samples compared to one another, corresponding NRT controls, and No Template Controls (NTC) for each primer pair using Luna Universal qCPR Master Mix (NEB, Ipswich, MA, #M3003) following the manufacturer’s protocol (1 µl cDNA per 10 µl reaction). A CFX384 Touch Real-Time PCR Detection System (BIO-RAD) was used for cDNA amplification and detection of FAM/SYBR Green fluorescence. qRT-PCR run data was analyzed using BIO-RAD CFX Manager software. Primer sequences are provided in Supplementary file 5. Primer binding efficiencies were analyzed over a wide range of genomic DNA template concentrations. We note that primers against 0D, 30D, 50D, 60Dv1, 70D, 100D, 50C5’, and mGFP100Dv1 had near identical binding efficiencies, whereas primers against 60Dv2, 60Dv3, 80D, 90D, 50C3’, 60C3’, 70C3’, 80C3’, and 90C3’ had slightly higher binding efficiencies. Amplification curves for all samples had characteristic S-shapes and each qPCR primer pair yielded a single melting peak. All cDNA samples reached threshold several cycles earlier than NRT and NTC controls, except whole larval 80D, which was within one cycle of the detection limit. For detection of tissue-specific mGFP100Dv1 mRNA levels, three technical replicates for all samples yielded very consistent CT values. A single testis sample, however, yielded an outlier delta CT value (mGFP100Dv1 minus GFP0D, Tukey’s multiple comparisons, p<0.0001), and was removed from the analysis after bleach-agarose electrophoresis revealed that this sample lacked detectable RNA. For tissue-specific detection of RpL10Aa-Endo and RpL10Aa-Com, and for whole larvae detection of GFP reporters, two technical replicates yielded consistent CT values. Relative transcript abundance was calculated using the 2^∆CT method to internally normalize expression of the indicated GFP reporters or RpL10Aa-Endo against the expression of the respective codon optimized transgene. Graphs were generated using GraphPad Prism 9.2.0.

Mean relative translation efficiency was calculated by dividing the average protein abundance (normalized to GFP0D) by the average mRNA abundance (normalized to GFP0D) and converting to a percentage. Only average values were used in the calculation, because protein and mRNA data were not obtained from paired samples.

Whole larval screening and image acquisition was on either a Zeiss SteREO Discovery.V12 (Zeiss Achromat S 0.63 x FWD 107 mm objective) and Zeiss AxioCam ICc 5 camera (images in Figure 1), or on a Leica MZ10 F stereoscope (Leica Plan APO 1.0 x objective #10450028) and Zeiss AxioCam MRc r2.1 camera (images in Figure 2). For larval screening, we assayed live larvae (a minimum of 50 larvae per line were examined in the screen). To immobilize larvae for imaging, WL3 larvae were anesthetized using di-ethyl ether for 3.5 min as described in Kakanj et al., 2020. Larval and adult tissues were prepared for imaging by dissecting in 1 x PBS, fixing in 1 x PBS, 3.7% paraformaldehyde, 0.3% Triton-X for 30 min, and staining with Hoechst 33,342 (1:1500, Life Technologies, Carlsbad, CA, #c10339). Tissues were mounted in Vectashield (Vector Laboratories Inc, Burlingame, CA). Tissue images were acquired on an upright Zeiss AxioImager M.2 without Apotome processing and with the Apotome unit positioned out of the light path (Zeiss 10 x NA 0.3 EC Plan-Neofluar objective) using a Zeiss Axiocam 503 mono version 1.1. Imaging conditions were identical between tissues to be visually compared (noted in the figure legends). Image processing was performed in FIJI (formatting) and Photoshop (applying levels adjustment layer uniformly across panels to be visually compared).

3’ Rapid Amplification of cDNA Ends (3’ RACE) was performed using the Roche 5’/3’ RACE kit, 2^nd generation (Roche Holding AG, Basel, Switzerland, cat# 03353621001) following the manufacturer’s protocol. RNA from whole WL3 larvae expressing GFP0D was used as a template. The template-specific SP5 primer sequence used was 5’-CCACAAGCTGGAGTACAACTACAACAGC-3’. Products from the 3’ RACE reaction were sequenced by Eurofins Genomics (Eurofins Genomics, Louisville, KY) using the following primer 5’-CGATAACCACTACCTGAGCACCC-3’.

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

The following kindly provided reagents used in this study: Bloomington Drosophila Stock Center, Developmental Studies Hybridoma Bank. David MacAlpine (Duke) provided valuable technical guidance for RT-PCR. Christopher Nicchitta (Duke) and William Marzluff (UNC Chapel Hill) provided valuable technical guidance for determining transgenic UTRs. Braden Tierney (Cornell) provided technical support in downloading FlyAtlas2 data. We thank Daniel Lew and Zhao Zhang (Duke) for comments on the manuscript. This project was supported by ACS grant RSG-128945 to DF, an NSF GRFP grant to SA, NIH grants R01CA94184 and P01CA203657 to CMC, and NIHG grants R35 GM140844 and R01 HL111527 to AL.

© 2022, Allen et al.

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