Survival of mineral-bound peptides into the Miocene
- Department of Life Sciences and Systems Biology, University of Turin, Italy
- The Globe Institute, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
- The Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Denmark
- Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy of Sciences, China
- McDonald Institute for Archaeological Research, University of Cambridge, United Kingdom
- Department of Geological Sciences, The University of Texas at Austin, United States
Figures
Figure 1
Map showing the location of the fossil eggshell site.
Figure 2
Eggshell specimen (IVPP V26107): photographs (A, B) and CT scan (C).
Figure 3 with 10 supplements
Annotated product ion spectrum of peptide ALDDDDYPKG, m/z 554.749, −10lgP=32.57.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 3—figure supplement 1
Annotated product ion spectrum, ALDDDDYPK, m/z 526.236, –10lgP=24.83.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 3—figure supplement 2
Annotated product ion spectrum, SALDDDDYPKG, m/z 598.266, −10lgP=21.11.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 3—figure supplement 3
Annotated product ion spectrum, DDDDYPKGKH, m/z 397.178, −10lgP=30.65.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 3—figure supplement 4
Annotated product ion spectrum, LDDDDYPKGK, m/z 583.277, −10lgP=30.91.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 3—figure supplement 5
Annotated product ion spectrum, SALDDDDYPK, m/z 569.754, −10lgP=29.94.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 3—figure supplement 6
Annotated product ion spectrum, DDDDYPKGK, m/z=526.734, −10lgP=28.62.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 3—figure supplement 7
Annotated product ion spectrum, LDDDDYPKG, m/z=519.23, −10lgP=16.57.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 3—figure supplement 8
Annotated product ion spectrum, DDDYPKGK, m/z=469.221, −10lgP=27.8.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 3—figure supplement 9
Annotated product ion spectrum, DDDDYPK, m/z=434.174, −10lgP=21.18.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 3—figure supplement 10
Annotated product ion spectrum, DDYPKGK, m/z=411.706, −10lgP=17.39.
Figure created using http://www.interactivepeptidespectralannotator.com/PeptideAnnotator.html (Brademan et al., 2019).
Figure 4
Coverage of struthiocalcin-1 (SCA-1) in Miocene OES specimen IVPP V26107.
Numbers indicate spectrum matches for each position in the sequence.
Author response image 1
Scatterplot of PEAKS peptide score versus precursor mass error.
Tables
Table 1
Peptide sequences identified in sample IVPP V26107.
| Scan | Precursor mass | z | m/z | Peptide sequence | Peptide mass | Error (ppm) | Peptide –10lgP |
|---|---|---|---|---|---|---|---|
| 5401 | 1107.472 | 2 | 555.246 | ALDDDDYPKG | 1107.472 | 5.7 | 27.24 |
| 5268 | 1050.457 | 2 | 526.236 | ALDDDDYPK | 1050.451 | 6.1 | 24.83 |
| 5051 | 1194.517 | 2 | 598.266 | SALDDDDYPKG | 1194.504 | 10.9 | 21.11 |
| 4984 | 1137.494 | 2 | 569.754 | SALDDDDYPK | 1137.483 | 10.1 | 29.94 |
| 4727 | 1137.494 | 2 | 569.755 | SALDDDDYPK | 1137.483 | 9.7 | 16.76 |
| 4565 | 1107.483 | 2 | 554.749 | ALDDDDYPKG | 1107.472 | 9.8 | 32.57 |
| 4565 | 1107.484 | 2 | 554.749 | ALDDDDYPKG | 1107.472 | 10.6 | 28.7 |
| 4443 | 1050.459 | 2 | 526.237 | ALDDDDYPK | 1050.451 | 7.6 | 17.62 |
| 4313 | 1050.461 | 2 | 526.235 | ALDDDDYPK | 1050.451 | 9.9 | 15.13 |
| 4199 | 1050.458 | 2 | 526.236 | ALDDDDYPK | 1050.451 | 7.2 | 16.35 |
| 4037 | 1036.442 | 2 | 519.230 | LDDDDYPKG | 1036.435 | 7.2 | 16.57 |
| 2724 | 1164.539 | 2 | 583.277 | LDDDDYPKGK | 1164.53 | 8.2 | 30.91 |
| 2502 | 866.335 | 2 | 434.176 | DDDDYPK | 866.335 | 6.9 | 18.46 |
| 2176 | 866.335 | 2 | 434.175 | DDDDYPK | 866.335 | 7.1 | 21.18 |
| 2026 | 866.338 | 2 | 434.176 | DDDDYPK | 866.329 | 10.1 | 19.07 |
| 1396 | 1051.454 | 2 | 526.734 | DDDDYPKGK | 1051.446 | 7.8 | 28.62 |
| 1138 | 936.427 | 2 | 469.221 | DDDYPKGK | 936.419 | 8.4 | 27.8 |
| 1046 | 1188.513 | 3 | 397.178 | DDDDYPKGKH | 1188.505 | 7.2 | 30.65 |
| 1042 | 821.398 | 2 | 411.706 | DDYPKGK | 821.392 | 7.6 | 17.39 |
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Survival of mineral-bound peptides into the Miocene
eLife 11:e82849.
https://doi.org/10.7554/eLife.82849
