Core PCP mutations affect short time mechanical properties but not tissue morphogenesis in the Drosophila pupal wing
Abstract
How morphogenetic movements are robustly coordinated in space and time is a fundamental open question in biology. We study this question using the wing of Drosophila melanogaster, an epithelial tissue that undergoes large-scale tissue flows during pupal stages. Previously, we showed that pupal wing morphogenesis involves both cellular behaviors that allow relaxation of mechanical tissue stress, as well as cellular behaviors that appear to be actively patterned (Etournay et al., 2015). Here, we show that these active cellular behaviors are not guided by the core planar cell polarity (PCP) pathway, a conserved signaling system that guides tissue development in many other contexts. We find no significant phenotype on the cellular dynamics underlying pupal morphogenesis in mutants of core PCP. Furthermore, using laser ablation experiments, coupled with a rheological model to describe the dynamics of the response to laser ablation, we conclude that while core PCP mutations affect the fast timescale response to laser ablation they do not significantly affect overall tissue mechanics. In conclusion, our work shows that cellular dynamics and tissue shape changes during Drosophila pupal wing morphogenesis do not require core PCP as an orientational guiding cue.
Data availability
Source data and code are provided for each figure
Article and author information
Author details
Funding
Max Planck Society
- Romina Piscitello-Gómez
- Franz S Gruber
- Abhijeet Krishna
- Charlie Duclut
- Carl D Modes
- Marko Popović
- Frank Jülicher
- Natalie A Dye
- Suzanne Eaton
Deutsche Forschungsgemeinschaft (EXC-2068-390729961)
- Romina Piscitello-Gómez
- Abhijeet Krishna
- Carl D Modes
- Frank Jülicher
- Natalie A Dye
- Suzanne Eaton
Deutsche Forschungsgemeinschaft (SPP1782)
- Romina Piscitello-Gómez
- Franz S Gruber
- Natalie A Dye
- Suzanne Eaton
Deutsche Krebshilfe (MSNZ-P2 Dresden)
- Natalie A Dye
Austrian Academy of Sciences (DOC Fellowship)
- Franz S Gruber
Agence Nationale de la Recherche (ANR-11-LABX-0071)
- Charlie Duclut
Agence Nationale de la Recherche (ANR-18-IDEX-0001)
- Charlie Duclut
Deutsche Forschungsgemeinschaft (EA4/10-1,EA4/10-2)
- Romina Piscitello-Gómez
- Franz S Gruber
- Natalie A Dye
- Suzanne Eaton
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Copyright
© 2023, Piscitello-Gómez et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
Metrics
-
- 790
- views
-
- 170
- downloads
-
- 1
- citations
Views, downloads and citations are aggregated across all versions of this paper published by eLife.
Download links
Downloads (link to download the article as PDF)
Open citations (links to open the citations from this article in various online reference manager services)
Cite this article (links to download the citations from this article in formats compatible with various reference manager tools)
Further reading
-
- Developmental Biology
Human autonomic neuronal cell models are emerging as tools for modeling diseases such as cardiac arrhythmias. In this systematic review, we compared 33 articles applying 14 different protocols to generate sympathetic neurons and 3 different procedures to produce parasympathetic neurons. All methods involved the differentiation of human pluripotent stem cells, and none employed permanent or reversible cell immortalization. Almost all protocols were reproduced in multiple pluripotent stem cell lines, and over half showed evidence of neural firing capacity. Common limitations in the field are a lack of three-dimensional models and models that include multiple cell types. Sympathetic neuron differentiation protocols largely mirrored embryonic development, with the notable absence of migration, axon extension, and target-specificity cues. Parasympathetic neuron differentiation protocols may be improved by including several embryonic cues promoting cell survival, cell maturation, or ion channel expression. Moreover, additional markers to define parasympathetic neurons in vitro may support the validity of these protocols. Nonetheless, four sympathetic neuron differentiation protocols and one parasympathetic neuron differentiation protocol reported more than two-thirds of cells expressing autonomic neuron markers. Altogether, these protocols promise to open new research avenues of human autonomic neuron development and disease modeling.
-
- Cell Biology
- Developmental Biology
A study in mice reveals key interactions between proteins involved in fibroblast growth factor signaling and how they contribute to distinct stages of eye lens development.