Early prediction of in-hospital death of COVID-19 patients: a machine-learning model based on age, blood analyses, and chest x-ray score
- University of Brescia, Italy
Abstract
An early-warning model to predict in-hospital mortality on admission of COVID-19 patients at an emergency department (ED) was developed and validate using a Machine-Learning model. In total, 2782 patients were enrolled between March 2020 and December 2020, including 2106 patients (first wave) and 676 patients (second wave) in the COVID-19 outbreak in Italy. The first-wave patients were divided into two groups with 1474 patients used to train the model, and 632 to validate it. The 676 patients in the second wave were used to test the model. Age, 17 blood analytes and Brescia chest X-ray score were the variables processed using a Random Forests classification algorithm to build and validate the model. ROC analysis was used to assess the model performances. A web-based death-risk calculator was implemented and integrated within the Laboratory Information System of the hospital. The final score was constructed by age (the most powerful predictor), blood analytes (the strongest predictors were lactate dehydrogenase, D-dimer, Neutrophil/Lymphocyte ratio, C-reactive protein, Lymphocyte %, Ferritin std and Monocyte %), and Brescia chest X-ray score. The areas under the receiver operating characteristic curve obtained for the three groups (training, validating and testing) were 0.98, 0.83 and 0.78, respectively. The model predicts in-hospital mortality on the basis of data that can be obtained in a short time, directly at the ED on admission. It functions as a web-based calculator, providing a risk score which is easy to interpret. It can be used in the triage process to support the decision on patient allocation.
Data availability
We are unable to share the dataset as it contains sensitive personal data collected during the pandemic at Spedali Civili di Brescia. We cannot share the full data since are data from patients. Interested researchers should contact the authors for any query related to data sharing and submit a project proposal Once defined the goal of the study, and the data needed authors will submit the potential project of collaboration to the IRB of Spedali Civili di Brescia to receive approval to access a deidentified dataset. Please note that other informations related to patients can be acquired, always following approoval of IRB of Spedali Civili di Brescia, not only the ones studied in the paper.Anyway, following request to the authors, it will be possible to share processed version of the dataset ( e.g. an Excel sheet with numbers used to plot the graphs and charts of the manuscript).All code used to analyse the data can be found on GitHub at https://github.com/biostatUniBS/BS_EWS
Article and author information
Author details
Funding
Stefano Calza (PRIN 20178S4EK9)
- Stefano Calza
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
Ethics
Human subjects: The Institutional review board aprpoved the study with the entry code NP4000.
Copyright
© 2021, Garrafa et al.
This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.
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Early prediction of in-hospital death of COVID-19 patients: a machine-learning model based on age, blood analyses, and chest x-ray score
eLife 10:e70640.
https://doi.org/10.7554/eLife.70640
Further reading
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The two identical motor domains (heads) of dimeric kinesin-1 move in a hand-over-hand process along a microtubule, coordinating their ATPase cycles such that each ATP hydrolysis is tightly coupled to a step and enabling the motor to take many steps without dissociating. The neck linker, a structural element that connects the two heads, has been shown to be essential for head–head coordination; however, which kinetic step(s) in the chemomechanical cycle is ‘gated’ by the neck linker remains unresolved. Here, we employed pre-steady-state kinetics and single-molecule assays to investigate how the neck-linker conformation affects kinesin’s motility cycle. We show that the backward-pointing configuration of the neck linker in the front kinesin head confers higher affinity for microtubule, but does not change ATP binding and dissociation rates. In contrast, the forward-pointing configuration of the neck linker in the rear kinesin head decreases the ATP dissociation rate but has little effect on microtubule dissociation. In combination, these conformation-specific effects of the neck linker favor ATP hydrolysis and dissociation of the rear head prior to microtubule detachment of the front head, thereby providing a kinetic explanation for the coordinated walking mechanism of dimeric kinesin.
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