The kleisin subunit controls the function of C. elegans meiotic cohesins by determining the mode of DNA binding and differential regulation by SCC-2 and WAPL-1

Abstract

The cohesin complex plays essential roles in chromosome segregation, 3D genome organisation, and DNA damage repair through its ability to modify DNA topology. In higher eukaryotes, meiotic chromosome function, and therefore fertility, requires cohesin complexes containing meiosis-specific kleisin subunits: REC8 and RAD21L in mammals and REC-8 and COH-3/4 in C. elegans. How these complexes perform the multiple functions of cohesin during meiosis and whether this involves different modes of DNA binding or dynamic association with chromosomes is poorly understood. Combining time-resolved methods of protein removal with live imaging and exploiting the temporospatial organisation of the C. elegans germline, we show that REC-8 complexes provide sister chromatid cohesion (SCC) and DNA repair, while COH-3/4 complexes control higher-order chromosome structure. High-abundance COH-3/4 complexes associate dynamically with individual chromatids in a manner dependent on cohesin loading (SCC-2) and removal (WAPL-1) factors. In contrast, low-abundance REC-8 complexes associate stably with chromosomes, tethering sister chromatids from S-phase until the meiotic divisions. Our results reveal that kleisin identity determines the function of meiotic cohesin by controlling the mode and regulation of cohesin-DNA association, and are consistent with a model in which SCC and DNA looping are performed by variant cohesin complexes that coexist on chromosomes.

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All data generated or analysed during this study are included in the manuscript and supporting files; source data for all graphs on the manuscript are provided in source data files associated with each figure containing graphs

Article and author information

Author details

  1. Maikel Castellano-Pozo

    MRC London Institute of Medical Sciences, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  2. Georgios Sioutas

    MRC London Institute of Medical Sciences, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  3. Consuelo Barroso

    MRC London Institute of Medical Sciences, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  4. Josh P Prince

    MRC London Institute of Medical Sciences, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0003-0877-7538
  5. Pablo Lopez-Jimenez

    Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0002-6673-5996
  6. Joseph Davy

    MRC London Institute of Medical Sciences, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  7. Angel-Luis Jaso-Tamame

    MRC London Institute of Medical Sciences, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  8. Oliver Crawley

    MRC London Institute of Medical Sciences, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  9. Nan Shao

    MRC London Institute of Medical Sciences, London, United Kingdom
    Competing interests
    The authors declare that no competing interests exist.
  10. Jesus Page

    Department of Biology, Universidad Autónoma de Madrid, Madrid, Spain
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-8381-324X
  11. Enrique Martinez-Perez

    MRC London Institute of Medical Sciences, London, United Kingdom
    For correspondence
    enrique.martinez-perez@imperial.ac.uk
    Competing interests
    The authors declare that no competing interests exist.
    ORCID icon "This ORCID iD identifies the author of this article:" 0000-0001-5813-0383

Funding

Medical Research Council (MC-A652-5PY60)

  • Enrique Martinez-Perez

European Molecular Biology Organization (Postdoctoral Fellowship)

  • Maikel Castellano-Pozo

Fundacion Alfonso Martin Escudero (Postdoctoral Fellowship)

  • Maikel Castellano-Pozo

European Molecular Biology Organization (Scientific exchange grant)

  • Pablo Lopez-Jimenez

Universidad Autonoma de Madrid

  • Jesus Page

The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication.

Copyright

© 2023, Castellano-Pozo et al.

This article is distributed under the terms of the Creative Commons Attribution License permitting unrestricted use and redistribution provided that the original author and source are credited.

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  1. Maikel Castellano-Pozo
  2. Georgios Sioutas
  3. Consuelo Barroso
  4. Josh P Prince
  5. Pablo Lopez-Jimenez
  6. Joseph Davy
  7. Angel-Luis Jaso-Tamame
  8. Oliver Crawley
  9. Nan Shao
  10. Jesus Page
  11. Enrique Martinez-Perez
(2023)
The kleisin subunit controls the function of C. elegans meiotic cohesins by determining the mode of DNA binding and differential regulation by SCC-2 and WAPL-1
eLife 12:e84138.
https://doi.org/10.7554/eLife.84138

Share this article

https://doi.org/10.7554/eLife.84138

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