September 2022

Research articles

1. 1. Developmental Biology
   2. Stem Cells and Regenerative Medicine

   Airway basal cells show regionally distinct potential to undergo metaplastic differentiation

   Yizhuo Zhou, Ying Yang ... Wellington V Cardoso

   A combination of unbiased scRNA-sequencing and functional approaches in organoid cultures and injury-repair models in vivo reveals major heterogeneity in adult murine airway basal cells, which is established prenatally and conserved in humans.
2. 1. Biochemistry and Chemical Biology
   2. Cell Biology

   Identification of orphan ligand-receptor relationships using a cell-based CRISPRa enrichment screening platform

   Dirk H Siepe, Lukas T Henneberg ... K Christopher Garcia

   A proteo-genomic CRISPR activation screening workflow to accelerate the identification of novel interactions between the secreted and membrane proteome with the potential to define new biological processes and identify future therapeutic targets.
3. 1. Immunology and Inflammation

   A neutrophil–B-cell axis impacts tissue damage control in a mouse model of intraabdominal bacterial infection via Cxcr4

   Riem Gawish, Barbara Maier ... Sylvia Knapp

   LPS pre-exposure is tissue protective during a subsequent lethal E. coli peritonitis by long-term modulation the bone marrow B-cell and neutrophil pool which affects neutrophil tissue damaging properties and therapeutic activation of Cxcr4 promotes tissue damage control during sepsis.
4. 1. Stem Cells and Regenerative Medicine

   In vivo generation of bone marrow from embryonic stem cells in interspecies chimeras

   Bingqiang Wen, Guolun Wang ... Vladimir V Kalinichenko

   Blastocyst complementation of rat embryos with donor mouse embryonic stem cells was used to simultaneously produce multiple, donor-derived hematopoietic and stromal cells in the interspecies bone marrow that exhibited normal gene expression signatures, cell surface markers, and functional characteristics.
5. 1. Evolutionary Biology
   2. Genetics and Genomics

   Protein evidence of unannotated ORFs in Drosophila reveals diversity in the evolution and properties of young proteins

   Eric B Zheng, Li Zhao

   The analysis of mass-spectrometry data for all possible open reading frames reveals protein evidence for evolutionarily young, unannotated proteins with distinct characters.
6. 1. Computational and Systems Biology

   Evolution of cell size control is canalized towards adders or sizers by cell cycle structure and selective pressures

   Felix Proulx-Giraldeau, Jan M Skotheim, Paul François

   An evolutionary algorithm is used to build gene networks implementing cell size control, and suggests multiple ways for evolution to first build sizers and turn them into adders depending on evolutionary constraints.
7. 1. Computational and Systems Biology
   2. Neuroscience

   Olfactory responses of Drosophila are encoded in the organization of projection neurons

   Kiri Choi, Won Kyu Kim, Changbong Hyeon

   The organization of olfactory projection neurons and their synaptic connectivity with third-order neurons studied along with olfactory responses suggest that the labeled-line design is partially at work in the Drosophila olfactory system.
8. 1. Neuroscience
   2. Structural Biology and Molecular Biophysics

   Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis

   Tianzhi Li, Qiqi Cheng ... Cong Ma

   A new binding mode between Rabphilin 3A and SNAP-25 enables efficient SNARE complex assembly via inducing a conformation change in SNAP-25 SNARE motif.
9. 1. Neuroscience

   Putting perception into action with inverse optimal control for continuous psychophysics

   Dominik Straub, Constantin A Rothkopf

   A new method for analyzing continuous psychophysics experiments estimates not only perceptual uncertainty from tracking tasks but also action variability, intrinsic costs, and subjective internal models.
10. 1. Immunology and Inflammation

    Bronchus-associated macrophages efficiently capture and present soluble inhaled antigens and are capable of local Th2 cell activation

    Xin-Zi Tang, Lieselotte S M Kreuk ... Christopher D C Allen

    Lung interstitial macrophages are strategically positioned underneath the bronchial airway epithelium and enriched at airway bifurcations for immunosurveillance of the conducting airway lumen and the initiation of adaptive immune responses.
11. 1. Structural Biology and Molecular Biophysics

    The Candida albicans virulence factor candidalysin polymerizes in solution to form membrane pores and damage epithelial cells

    Charles M Russell, Katherine G Schaefer ... Francisco N Barrera

    The molecular mechanism that candidalysin uses to perforate membranes is unraveled, which opens the door to the rational design of inhibitors against candidiasis.
12. 1. Immunology and Inflammation

    Macrophage innate training induced by IL-4 and IL-13 activation enhances OXPHOS driven anti-mycobacterial responses

    Mimmi LE Lundahl, Morgane Mitermite ... Ed C Lavelle

    Type 2 cytokines induce macrophage innate training, where enhanced pro-inflammatory responses are fuelled by oxidative phosphorylation rather than aerobic glycolysis.
13. 1. Neuroscience

    Functional brain reconfiguration during sustained pain

    Jae-Joong Lee, Sungwoo Lee ... Choong-Wan Woo

    Human neuroimaging with time-evolving network analysis reveals dynamic interactions among multiple functional brain networks in response to sustained pain.
14. 1. Cell Biology

    Phosphoproteomic mapping reveals distinct signaling actions and activation of muscle protein synthesis by Isthmin-1

    Meng Zhao, Niels Banhos Danneskiold-Samsøe ... Katrin J Svensson

    The role of Isthmin-1 in muscle function is defined by using phosphoproteomics to identify distinct and overlapping signaling pathways between Isthmin-1 and insulin.
15. 1. Developmental Biology
    2. Neuroscience

    Plexins promote Hedgehog signaling through their cytoplasmic GAP activity

    Justine M Pinskey, Tyler M Hoard ... Benjamin L Allen

    The plexin family of semaphorin receptors regulate Hedgehog pathway activity via a conserved GAP domain and FYN kinase phosphorylation site in their cytoplasmic domain.
16. 1. Computational and Systems Biology

    Ear pinnae in a neotropical katydid (Orthoptera: Tettigoniidae) function as ultrasound guides for bat detection

    Christian A Pulver, Emine Celiker ... Fernando Montealegre-Z

    Located in the legs, the miniaturized katydid ears exhibit cuticular pinnae to only capture high-ultrasonic bat echolocation calls, but katydid also hear their own calls using alternative ear paths, which suggest that their ears operate in a colossal frequency range.
17. 1. Neuroscience

    Distinct population and single-neuron selectivity for executive and episodic processing in human dorsal posterior cingulate

    Lyndsey Aponik-Gremillion, Yvonne Y Chen ... Brett L Foster

    Invasive recordings from human dorsal posterior cingulate cortex show neural population responses occur for only executive tasks, while single neuron responses occur for specific executive or episodic cognitive tasks.
18. 1. Neuroscience

    Functional gradients in the human lateral prefrontal cortex revealed by a comprehensive coordinate-based meta-analysis

    Majd Abdallah, Gaston E Zanitti ... Demian Wassermann

    A comprehensive meta-analysis of the neuroimaging literature reveals that the lateral prefrontal cortex of humans is mainly organized along its rostrocaudal axis according to a unimodal-to-transmodal pattern of network connectivity and a concrete-to-abstract axis of functional associations.
19. 1. Cell Biology

    De novo apical domain formation inside the Drosophila adult midgut epithelium

    Jia Chen, Daniel St Johnston

    Drosophila enteroblasts form septate junctions, an apical domain and an internal lumen inside the midgut epithelium before they reach the gut surface, thereby maintaining the intestinal barrier as they integrate.
20. 1. Chromosomes and Gene Expression

    The SPARC complex defines RNAPII promoters in Trypanosoma brucei

    Desislava P Staneva, Stefan Bresson ... Robin C Allshire

    The SPARC complex directs accurate RNAPII positioning for transcription initiation and, in some instances, ensures correct transcription directionality in Trypanosoma brucei.
21. 1. Neuroscience

    Time encoding migrates from prefrontal cortex to dorsal striatum during learning of a self-timed response duration task

    Gabriela C Tunes, Eliezyer Fermino de Oliveira ... Marcelo Bussotti Reyes

    Electrophysiological and pharmacological data provide evidence that the medial prefrontal cortex is essential for rats to learn a timing task, becoming unnecessary later, while the dorsal striatum does not play a role initially, taking over when animals become proficient.
22. 1. Neuroscience

    Flexing the principal gradient of the cerebral cortex to suit changing semantic task demands

    Zhiyao Gao, Li Zheng ... Elizabeth Jefferies

    Gradient decomposition of informational connectivity reveals that the principal gradient with the separation of default mode network from sensory-motor systems represents a hallmark of the retrieval of strong conceptual links.
23. 1. Developmental Biology
    2. Neuroscience

    A dynamic and expandable digital 3D-atlas maker for monitoring the temporal changes in tissue growth during hindbrain morphogenesis

    Matthias Blanc, Giovanni Dalmasso ... Cristina Pujades

    The digital 3D-atlas maker will help ascribing neuronal birthdate and neuronal differentiation times upon gene disruption in zebrafish avatars for human diseases, and will allow the integration of the information generated in other laboratories.
24. 1. Developmental Biology

    Integration of mouse ovary morphogenesis with developmental dynamics of the oviduct, ovarian ligaments, and rete ovarii

    Jennifer McKey, Dilara N Anbarci ... Blanche Capel

    A detailed characterization of mouse ovary development using tissue clearing and 3D lightsheet imaging identifies ovary folding, specification of cortical and medullary domains, and ovary encapsulation as major hallmarks of ovary morphogenesis.
25. 1. Ecology
    2. Evolutionary Biology

    Species clustering, climate effects, and introduced species in 5 million city trees across 63 US cities

    Dakota E McCoy, Benjamin Goulet-Scott ... John Kartesz

    Across 63 large US cities, street tree communities are shaped by climate, clustered by species, and made more similar between cities due to the presence of introduced species.
26. 1. Neuroscience

    Insulin sensitivity in mesolimbic pathways predicts and improves with weight loss in older dieters

    Lena J Tiedemann, Sebastian M Meyhöfer ... Stefanie Brassen

    Insulinergic neuromodulation of hedonic food processing predicts future weight management and improves with weight loss in older overweight dieters.
27. 1. Neuroscience

    Mixed synapses reconcile violations of the size principle in zebrafish spinal cord

    Evdokia Menelaou, Sandeep Kishore, David L McLean

    In the zebrafish spinal cord, mixed synapses contribute to neuronal connectivity and resting excitability, which helps explain violations of the well-known 'size principle' of recruitment.
28. 1. Epidemiology and Global Health

    The effect of calcium supplementation in people under 35 years old: A systematic review and meta-analysis of randomized controlled trials

    Yupeng Liu, Siyu Le ... Shuran Wang

    A systematic review and meta-analysis show that calcium supplementation significantly improves bone mass, implying that preventive calcium supplementation in young people (age ≤35 years) may be a shift in the window of intervention for osteoporosis.
29. 1. Neuroscience

    Conduction velocity along a key white matter tract is associated with autobiographical memory recall ability

    Ian A Clark, Siawoosh Mohammadi ... Eleanor A Maguire

    A new magnetic resonance brain imaging measure reveals that variations in people’s ability to recall their past experiences may be related to the speed at which electrical signals travel along axons in the parahippocampal cingulum bundle.
30. 1. Cell Biology
    2. Genetics and Genomics

    Resurrecting essential amino acid biosynthesis in mammalian cells

    Julie Trolle, Ross M McBee ... Harris H Wang

    Mammalian cells were engineered to synthesize valine, a metabolic capacity that had been lost from the lineage of higher eukaryotes for >500 million years.
31. 1. Epidemiology and Global Health

    Trends in female-selective abortion among Asian diasporas in the United States, United Kingdom, Canada and Australia

    Catherine Meh, Prabhat Jha

    While selective abortion of female fetuses is documented within China and India, it is also significant among Asian diaspora in Canada, US, UK and Australia, particularly among Indian diaspora for second or third births following earlier girl birth(s).
32. 1. Medicine

    Loss of full-length dystrophin expression results in major cell-autonomous abnormalities in proliferating myoblasts

    Maxime RF Gosselin, Virginie Mournetas ... Dariusz C Gorecki

    Loss of full-length dystrophin expression causes significant molecular and functional defects in human and mouse myoblast, thus closing the vicious cycle of DMD pathology.
33. 1. Neuroscience

    Uncertainty-based inference of a common cause for body ownership

    Marie Chancel, H Henrik Ehrsson, Wei Ji Ma

    Behavioral and computational results show that the perception of our body as our own depends on Bayesian probabilistic reasoning that take into account the variations in sensory uncertainty when integrating visual and somatosensory cues.
34. 1. Neuroscience

    Quantitative MRI reveals differences in striatal myelin in children with DLD

    Saloni Krishnan, Gabriel J Cler ... Kate E Watkins

    A new quantitative MRI protocol reveals reduced myelin in the striatum in children with developmental language disorder, shedding light on the brain basis of this disorder.
35. 1. Ecology

    Bumblebees retrieve only the ordinal ranking of foraging options when comparing memories obtained in distinct settings

    Cwyn Solvi, Yonghe Zhou ... Fei Peng

    Unlike humans and starlings which use memories of both absolute and relative information to decide between options in novel contexts, bumblebees rely only on the remembered ordinal ranking of options.
36. 1. Immunology and Inflammation
    2. Microbiology and Infectious Disease

    Post-phagocytosis activation of NLRP3 inflammasome by two novel T6SS effectors

    Hadar Cohen, Noam Baram ... Motti Gerlic

    A horizontally shared type VI secretion system affects the interaction between vibrios and eukaryotic hosts.
37. 1. Biochemistry and Chemical Biology

    Hepatic AMPK signaling dynamic activation in response to REDOX balance are sentinel biomarkers of exercise and antioxidant intervention to improve blood glucose control

    Meiling Wu, Anda Zhao ... Dongyun Shi

    Moderate exercise upregulated compensatory antioxidant capability and reached a high-level redox balance, whereas antioxidant intervention achieved a low-level redox balance by inhibiting oxidative stress for treating diabetes.
38. 1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Early anteroposterior regionalisation of human neural crest is shaped by a pro-mesodermal factor

    Antigoni Gogolou, Celine Souilhol ... Anestis Tsakiridis

    A thorough insight into the previously unrecognised role of a critical developmental regulator known as TBXT in influencing the specification of human trunk neural crest cells, the presumed precursors of the childhood tumour neuroblastoma.
39. 1. Evolutionary Biology
    2. Genetics and Genomics

    Polygenic adaptation after a sudden change in environment

    Laura Katharine Hayward, Guy Sella

    A shift in fitness optimum of a polygenic trait rapidly introduces small frequency differences between alleles with effects aligned with and opposing the shift, which gradually translate into small differences in fixation probability.
40. 1. Microbiology and Infectious Disease

    c-Myc plays a key role in IFN-γ-induced persistence of Chlamydia trachomatis

    Nadine Vollmuth, Lisa Schlicker ... Thomas Rudel

    The central immune modulator interferon-gamma downregulates the proto-oncogene c-Myc to shut down host cell metabolism and interfere with infection of epithelial cells by obligate intracellular pathogenic Chlamydia trachomatis.
41. 1. Cell Biology
    2. Developmental Biology

    Fat2 polarizes the WAVE complex in trans to align cell protrusions for collective migration

    Audrey Miller Williams, Seth Donoughe ... Sally Horne-Badovinac

    Fat2 signals from the trailing edge of each cell to localize WAVE activity to the leading edge of the cell behind, polarizing protrusive regions within cells and across the epithelium.
42. 1. Physics of Living Systems

    Self-organized canals enable long-range directed material transport in bacterial communities

    Ye Li, Shiqi Liu ... Yilin Wu

    Mechanochemical coupling between interfacial force and biosurfactant kinetics can coordinate large-scale material transport in primitive life forms, suggesting a new principle to engineer self-organized microbial communities.
43. 1. Cell Biology
    2. Genetics and Genomics

    Differential requirements for mitochondrial electron transport chain components in the adult murine liver

    Nicholas P Lesner, Xun Wang ... Prashant Mishra

    Animal models reveal that mitochondrial complex I is dispensable for homeostatic functions of the mouse liver.
44. 1. Developmental Biology
    2. Genetics and Genomics

    Fibroblast-derived Hgf controls recruitment and expansion of muscle during morphogenesis of the mammalian diaphragm

    Elizabeth M Sefton, Mirialys Gallardo ... Gabrielle Kardon

    HGF derived from fibroblasts is critical for complete muscularization of the mammalian diaphragm, and loss-of-function experiments reveal that partial muscularization of the diaphragm is not sufficient to cause congenital diaphragmatic hernias.
45. 1. Computational and Systems Biology
    2. Genetics and Genomics

    Rapid, Reference-Free human genotype imputation with denoising autoencoders

    Raquel Dias, Doug Evans ... Ali Torkamani

    A new autoencoder-based genotype imputation method shows superior accuracy across human genomes of diverse ancestry and across the allele-frequency spectrum, while delivering significantly faster inference run times relative to standard imputation tools.
46. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    A feed-forward pathway drives LRRK2 kinase membrane recruitment and activation

    Edmundo G Vides, Ayan Adhikari ... Suzanne R Pfeffer

    Parkinson’s disease-associated LRRK2 kinase is recruited to membranes by its Rab GTPase substrates, and LRRK2 is both retained on membranes and further activated there by cooperative interaction with the phosphorylated Rab proteins that it generates.
47. 1. Cell Biology
    2. Chromosomes and Gene Expression

    Wolbachia action in the sperm produces developmentally deferred chromosome segregation defects during the Drosophila mid-blastula transition

    Brandt Warecki, Simon William Abraham Titen ... William Sullivan

    Differentiation of mitotic errors occurring during late embryonic development from the well-characterized first division defects provides new insight into the mechanisms of Wolbachia-induced cytoplasmic incompatibility.
48. 1. Cell Biology

    Consequences of PDGFRα⁺ fibroblast reduction in adult murine hearts

    Jill T Kuwabara, Akitoshi Hara ... Michelle D Tallquist

    Cardiac fibroblast reduction is well-tolerated and demonstrates beneficial outcomes after heart injury.
49. 1. Developmental Biology

    Regulation of Nodal signaling propagation by receptor interactions and positive feedback

    Hannes Preiß, Anna C Kögler ... Patrick Müller

    A subset of the zebrafish Nodal receptor complex is regulated by positive feedback and controls the diffusion and distribution of Nodal signals during germ layer formation.
50. 1. Neuroscience

    Non-rapid eye movement sleep determines resilience to social stress

    Brittany J Bush, Caroline Donnay ... J Christopher Ehlen

    Pre-existing variability in the regulation of non-rapid eye movement sleep predicts resilience to social-defeat stress.
51. 1. Ecology
    2. Evolutionary Biology

    Mountain gorillas maintain strong affiliative biases for maternal siblings despite high male reproductive skew and extensive exposure to paternal kin

    Nicholas M Grebe, Jean Paul Hirwa ... Stacy Rosenbaum

    Mountain gorillas, who live in close-knit social groups with siblings and non-siblings of both sexes throughout their lives, show distinct behavioral biases towards maternal versus paternal kin.
52. 1. Developmental Biology
    2. Genetics and Genomics

    Variable paralog expression underlies phenotype variation

    Raisa Bailon-Zambrano, Juliana Sucharov ... James T Nichols

    Within populations, there is standing variation in paralog expression levels, paralog expression levels are heritable, and variation in paralog expression can modify the phenotypes associated with mutation of one of the paralogs.
53. 1. Evolutionary Biology
    2. Genetics and Genomics

    Fitness effects of CRISPR endonucleases in Drosophila melanogaster populations

    Anna M Langmüller, Jackson Champer ... Philipp W Messer

    In Drosophila, genomic CRISPR/Cas9 expression necessary for CRISPR-based gene drives harbors fitness costs presumably caused by off-target effects, which can be minimized by using a high-fidelity version of Cas9.
54. 1. Epidemiology and Global Health

    SARS-CoV-2 antibody dynamics in blood donors and COVID-19 epidemiology in eight Brazilian state capitals: A serial cross-sectional study

    Carlos A Prete Jr, Lewis F Buss ... Ester C Sabino

    Blood donor serosurveillance in eight of Brazil’s most populous cities reveals extensive variation of SARS-CoV-2 attack rate across cities, age, and sex groups in December 2020 and increased intrinsic severity of the Gamma variant of concern.
55. 1. Neuroscience

    Higher-order unimodal olfactory sensory preconditioning in Drosophila

    Juan Martinez-Cervantes, Prachi Shah ... Isaac Cervantes-Sandoval

    Drosophila flies infer value to olfactory stimuli based on the previously repeatedly presented associative structure between two odorants.
56. 1. Cancer Biology
    2. Computational and Systems Biology

    Proteogenomic analysis of cancer aneuploidy and normal tissues reveals divergent modes of gene regulation across cellular pathways

    Pan Cheng, Xin Zhao ... Teresa Davoli

    RNA-level regulation of gene expression is negatively associated with protein-level regulation across cellular pathways in normal tissues and cancer.
57. 1. Neuroscience

    A dopamine-gated learning circuit underpins reproductive state-dependent odor preference in Drosophila females

    Ariane C Boehm, Anja B Friedrich ... Ilona C Grunwald Kadow

    Behavioral and in vivo imaging analysis in the fly suggests that mating-related sensory experience regulates female odor perception and expression of choice behavior through a dopamine-gated learning circuit.
58. 1. Cell Biology
    2. Stem Cells and Regenerative Medicine

    Contribution of Trp63^CreERT2-labeled cells to alveolar regeneration is independent of tuft cells

    Huachao Huang, Yinshan Fang ... Jianwen Que

    A population of p63⁺ Krt5^- epithelial cells contribute to alveolar regeneration independent of the presence of ectopic tuft cells following viral infection.
59. 1. Epidemiology and Global Health
    2. Medicine

    The ellipse of insignificance, a refined fragility index for ascertaining robustness of results in dichotomous outcome trials

    David Robert Grimes

    An ellipse of insignificant analysis is a robust method for ascertaining the strength of even large dichotomous outcome trials in biomedical science, and is a novel means to detect potentially dubious results and research fraud.
60. 1. Medicine

    FSH-blocking therapeutic for osteoporosis

    Sakshi Gera, Tan-Chun Kuo ... Mone Zaidi

    The first-in-class, humanized, epitope-specific FSH-blocking antibody MS-Hu6 is efficacious, durable, and manufacturable, and is therefore poised for further development towards future testing in human trials for osteoporosis, obesity, dyslipidemia and neurodegeneration.
61. 1. Developmental Biology
    2. Genetics and Genomics

    Diverse ancestry whole-genome sequencing association study identifies TBX5 and PTK7 as susceptibility genes for posterior urethral valves

    Melanie MY Chan, Omid Sadeghi-Alavijeh ... Daniel P Gale

    A genome-wide association study using whole-genome sequencing data identifies the first known susceptibility genes for posterior urethral valves, the most common cause of kidney failure in boys, implicating the transcription factor TBX5 and planar cell polarity gene PTK7.
62. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    H3K9me1/2 methylation limits the lifespan of daf-2 mutants in C. elegans

    Meng Huang, Minjie Hong ... Xuezhu Feng

    The depletion of a new class of putative histone methyltransferases, including MET-2, SET-6, SET-19, SET-20, SET-21, SET-32, and SET-33, induced synergistic lifespan extension in daf-2 animals to an average lifespan nearly three times that of wild-type animals.
63. 1. Computational and Systems Biology

    Network design principle for robust oscillatory behaviors with respect to biological noise

    Lingxia Qiao, Zhi-Bo Zhang ... Lei Zhang

    Searching all two- and three-node gene regulatory network topologies identifies the repressilator with positive autoregulation as the motif capable of accurate oscillation and reveals the long period and high amplitude as two distinct mechanisms for buffering different noise.
64. 1. Cancer Biology
    2. Chromosomes and Gene Expression

    DNA methylome combined with chromosome cluster-oriented analysis provides an early signature for cutaneous melanoma aggressiveness

    Arnaud Carrier, Cécile Desjobert ... Paola B Arimondo

    An original molecular multi-step approach integrating DNA methylome and chromosome-cluster localization cross-analysis identified a DNA methylation signature of patient outcome supporting that the core DNA methylation for a given trait is independent of the physiological context under which it arises.
65. 1. Computational and Systems Biology

    Empirical single-cell tracking and cell-fate simulation reveal dual roles of p53 in tumor suppression

    Ann Rancourt, Sachiko Sato, Masahiko S Satoh

    Single-cell tracking and cell-fate simulation suggest that low levels of p53 suppress aneuploid cell formation in unstressed cells and also reveal the dual fate of cells lacking p53 in the presence of p53-proficient cells.
66. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Kinesin-1, -2, and -3 motors use family-specific mechanochemical strategies to effectively compete with dynein during bidirectional transport

    Allison M Gicking, Tzu-Chen Ma ... William O Hancock

    The motility of three different kinesin-dynein pairs were analyzed and found to be strikingly similar, suggesting that bidirectional transport is not regulated by motor type, but rather by scaffold, adapter, and regulatory proteins.
67. 1. Neuroscience

    Distance estimation from monocular cues in an ethological visuomotor task

    Philip RL Parker, Elliott TT Abe ... Cristopher M Niell

    Mice accurately perform an ethological distance estimation task based on gap jumping and can use monocular cues to estimate distance in addition to binocular cues such as stereopsis.
68. 1. Neuroscience

    Frequency-specific neural signatures of perceptual content and perceptual stability

    Richard Hardstone, Matthew W Flounders ... Biyu J He

    While perceptual content is encoded in non-oscillatory activity in the slow cortical potential (<5 Hz) range, perceptual stability is predominantly influenced by the amplitude fluctuations of alpha (~10 Hz) and beta (~20 Hz) brain oscillations.
69. 1. Neuroscience

    Repressor element 1-silencing transcription factor deficiency yields profound hearing loss through K_v7.4 channel upsurge in auditory neurons and hair cells

    Haiwei Zhang, Hongchen Li ... Ping Lv

    In the cochlea, repressor element 1-silencing transcription factor (REST) regulates the expression levels of K_v7.4 and alterations of REST functions result in progressive hearing loss.
70. 1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Global chromatin mobility induced by a DSB is dictated by chromosomal conformation and defines the HR outcome

    Fabiola García Fernández, Etienne Almayrac ... Emmanuelle Fabre

    A designed system to track homologous recombination (HR) in vivo demonstrates the importance of chromosome organization in the induction of global mobility in response to double-strand breaks and characterizes the role of two types of global motility in HR.
71. 1. Immunology and Inflammation
    2. Medicine

    Bone marrow adipocytes drive the development of tissue invasive Ly6C^high monocytes during obesity

    Parastoo Boroumand, David C Prescott ... Amira Klip

    Diet-induced obesity in mice led to a duration of diet-dependent bone marrow adipocyte whitening, which drove monocyte expansion and skewing towards a tissue-invasive phenotype, likely contributing to ensuing tissue infiltration that accompanies obesity and diabetes.
72. 1. Cancer Biology
    2. Immunology and Inflammation

    Transient cell-in-cell formation underlies tumor relapse and resistance to immunotherapy

    Amit Gutwillig, Nadine Santana-Magal ... Yaron Carmi

    Tumors escape killing by the immune system through generating transient spatial cell-in-cell structures that are impenetrable to cytotoxic compounds including lytic granules and chemotherapy.
73. 1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Group II truncated haemoglobin YjbI prevents reactive oxygen species-induced protein aggregation in Bacillus subtilis

    Takeshi Imai, Ryuta Tobe ... Hisaaki Mihara

    The repair of oxidatively damaged proteins by the newly discovered activity in YjbI is important for the adaptation of Bacillus subtilis to oxidative environments.
74. 1. Neuroscience

    Stability of motor representations after paralysis

    Charles Guan, Tyson Aflalo ... Richard A Andersen

    Neural populations in PPC dynamically represent motor-like and then sensory-like aspects of brain–computer interface finger movements with a representational structure that matches able-bodied individuals.
75. 1. Immunology and Inflammation
    2. Physics of Living Systems

    Progressive enhancement of kinetic proofreading in T cell antigen discrimination from receptor activation to DAG generation

    Derek M Britain, Jason P Town, Orion David Weiner

    A light-controllable ligand is used to probe where in the signaling cascade T cells discriminate self from non-self.
76. 1. Neuroscience

    A partially nested cortical hierarchy of neural states underlies event segmentation in the human brain

    Linda Geerligs, Dora Gözükara ... Umut Güçlü

    The ongoing stream of information that comes in through our senses is segmented into distinct neural states at each level of the cortical hierarchy, which underpins our experience of distinct events.
77. 1. Epidemiology and Global Health

    Linking rattiness, geography and environmental degradation to spillover Leptospira infections in marginalised urban settings: An eco-epidemiological community-based cohort study in Brazil

    Max T Eyre, Fábio N Souza ... Federico Costa

    While flooding events may drive spillover transmission of Leptospira spp. through dispersal of the bacteria at low elevations, at higher elevations environmental risk is more localised and directly driven by the distribution of local rat reservoir populations.
78. 1. Developmental Biology
    2. Neuroscience

    Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons

    Jennifer M Lin, Tyler A Mitchell ... Paolo Emanuele Forni

    In vivo genetic experiments show that aberrant expression of a transcription factor can compromise the functionality of neurons which is important to better understand the potential mechanisms underlying neuropathologies.
79. 1. Neuroscience

    Neural signatures of auditory hypersensitivity following acoustic trauma

    Matthew McGill, Ariel E Hight ... Daniel B Polley

    Chronic two-photon calcium imaging, optogenetics, and operant behavioral approaches were leveraged to show that hyperactivity, hyperresponsivity, and hypersynchronization in auditory cortex neural ensembles can account for auditory hypersensitivity following acoustic trauma.
80. 1. Cancer Biology
    2. Medicine

    Photonic hyperthermia of malignant peripheral nerve sheath tumors at the third near-infrared biowindow

    Yihui Gu, Zhichao Wang ... Qingfeng Li

    In vivo and in vitro experiments confirm the conspicuous antineoplastic efficacy of NIR-III photothermal therapy under the premise of ensuring biosafety.
81. 1. Neuroscience

    7-Dehydrocholesterol-derived oxysterols cause neurogenic defects in Smith-Lemli-Opitz syndrome

    Hideaki Tomita, Kelly M Hines ... Libin Xu

    A 7-dehydrocholesterol-derived oxysterol in SLOS was found to profoundly impact neurogenesis during cortical development by interacting with glucocorticoid receptor, which points to new therapeutic approaches for SLOS by targeting the activities of this oxysterol.
82. 1. Microbiology and Infectious Disease

    Mobilome-driven segregation of the resistome in biological wastewater treatment

    Laura de Nies, Susheel Bhanu Busi ... Paul Wilmes

    Biological wastewater treatment plants are critical reservoirs of antimicrobial resistance (AMR), allowing for early detection and monitoring of resistant pathogens, whilst serving as models for understanding the segregation of mobile genetic elements through AMR.
83. 1. Cell Biology
    2. Immunology and Inflammation

    Microtubules restrict F-actin polymerization to the immune synapse via GEF-H1 to maintain polarity in lymphocytes

    Judith Pineau, Léa Pinon ... Paolo Pierobon

    Microfluidics and dynamic imaging allow systematic characterization of polarization kinetics in B lymphocytes and highlight the role of microtubules as master regulators of actin polymerization at the immune synapse.
84. 1. Cancer Biology
    2. Cell Biology

    GCN2 eIF2 kinase promotes prostate cancer by maintaining amino acid homeostasis

    Ricardo A Cordova, Jagannath Misra ... Kirk A Staschke

    The ISR kinase GCN2 is critical for maintaining tumor amino acid levels to facilitate growth, suggesting a novel therapeutic strategy for the treatment of prostate cancer by inducing starvation for essential amino acids.
85. 1. Immunology and Inflammation
    2. Medicine

    Neutrophil-mediated fibroblast-tumor cell il-6/stat-3 signaling underlies the association between neutrophil-to-lymphocyte ratio dynamics and chemotherapy response in localized pancreatic cancer: A hybrid clinical-preclinical study

    Iago de Castro Silva, Anna Bianchi ... Jashodeep Datta

    A neutrophil-CAF-tumor cell IL-1β/IL-6/STAT-3 signaling axis in the pancreatic tumor microenvironment underlies the association between neutrophil-to-lymphocyte ratio dynamics and pathologic response in patients with pancreatic ductal adenocarcinoma undergoing neoadjuvant therapy.
86. 1. Cell Biology
    2. Chromosomes and Gene Expression

    Rvb1/Rvb2 proteins couple transcription and translation during glucose starvation

    Yang S Chen, Wanfu Hou ... Brian M Zid

    A new mechanism of Rvb1/Rvb2 proteins was found that couples transcription, mRNA granular localization and translation of select genes during glucose starvation stress.
87. 1. Neuroscience

    Shared mechanisms of auditory and non-auditory vocal learning in the songbird brain

    James N McGregor, Abigail L Grassler ... Samuel J Sober

    A novel, non-auditory learning paradigm reveals that songbirds can modify their vocal output based on somatosensory signals and that a common set of brain pathways underlies both this form of vocal learning and auditory-guided vocal learning.
88. 1. Developmental Biology

    Murine blastocysts generated by in vitro fertilization show increased Warburg metabolism and altered lactate production

    Seok Hee Lee, Xiaowei Liu ... Paolo F Rinaudo

    In vitro fertilization-conceived murine embryos show evidence of oxidative and metabolic stress with alteration in lactic acid metabolism.
89. 1. Neuroscience

    Balance between breadth and depth in human many-alternative decisions

    Alice Vidal, Salvador Soto-Faraco, Rubén Moreno-Bote

    A novel experimental paradigm investigating how humans manage limited search capacity over many alternatives reveals close-to-optimal behaviours and identifies heuristics used to minimize computation with little impact on performance.
90. 1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission

    Dawn M Wenzel, Douglas R Mackay ... Wesley I Sundquist

    A comprehensive, quantitative, protein-protein interaction screen maps the network of interactions between human MIT domains and ESCRT-III C-terminal tails, and reveals MIT domain-containing protein cofactors for abscission and the 'No Cut' abscission checkpoint.
91. 1. Biochemistry and Chemical Biology
    2. Cancer Biology

    Solute exchange through gap junctions lessens the adverse effects of inactivating mutations in metabolite-handling genes

    Stefania Monterisi, Johanna Michl ... Pawel Swietach

    Imaging studies of connexin-coupled networks of colorectal cancer cells reveal that cells carrying a genetic defect in an essential metabolic pathway can be rescued by diffusive exchange with neighboring wild-type cells via gap junctions, particularly Cx26, thereby evading negative selection.
92. 1. Immunology and Inflammation

    Memory persistence and differentiation into antibody-secreting cells accompanied by positive selection in longitudinal BCR repertoires

    Artem Mikelov, Evgeniia I Alekseeva ... Ivan V Zvyagin

    High degree of clonal persistence and excess of inter-individual convergence are observed in human memory B cell repertoires, along with signatures of both negative and positive selection in most abundant clonal lineages.
93. 1. Computational and Systems Biology
    2. Neuroscience

    Reverse engineering of metacognition

    Matthias Guggenmos

    A model framework and toolbox to quantify metacognitive biases and sources of metacognitive noise in animal and human confidence data.
94. 1. Developmental Biology

    A neurogenic signature involving monoamine Oxidase-A controls human thermogenic adipose tissue development

    Javier Solivan-Rivera, Zinger Yang Loureiro ... Silvia Corvera

    A hybrid mouse/human model using mesenchymal progenitor cells reveals dynamics of human adipose tissue development and mechanisms that may enhance human adipose thermogenic capacity.
95. 1. Neuroscience

    3D optogenetic control of arteriole diameter in vivo

    Philip J O'Herron, David A Hartmann ... Andy Y Shih

    The diameter of individual or multiple arterioles can be rapidly controlled with high spatiotemporal resolution using optogenetics, providing a technique for the direct manipulation of blood flow independently of neural activity or pharmacological agents.
96. 1. Ecology

    Experimental evidence that chronic outgroup conflict reduces reproductive success in a cooperatively breeding fish

    Ines Braga Goncalves, Andrew N Radford

    Outgroup conflict can negatively impact general reproductive behaviour, egg investment, and parental care, resulting in decreased reproductive output even in the absence of physical confrontations.
97. 1. Biochemistry and Chemical Biology
    2. Cell Biology

    Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway

    Tolulope Sokoya, Jan Parolek ... Joost CM Holthuis

    Organellar lipidomics and lipid reporter studies in intact cells reveal how disease-relevant mutations in a sphingolipid biosynthetic enzyme cause a wide-ranging perturbation of lipid distributions and membrane properties along the secretory pathway.
98. 1. Neuroscience

    Learning of probabilistic punishment as a model of anxiety produces changes in action but not punisher encoding in the dmPFC and VTA

    David S Jacobs, Madeleine C Allen ... Bita Moghaddam

    A novel approach to studying anxiety reveals that learned anxiety and diazepam treatment have unique roles on prefrontal cortex and ventral tegmental area activity.
99. 1. Neuroscience

    Robust group- but limited individual-level (longitudinal) reliability and insights into cross-phases response prediction of conditioned fear

    Maren Klingelhöfer-Jens, Mana R Ehlers ... Tina B Lonsdorf

    Reliability and predictability analyses beyond standard measures provide empirically based guidance regarding the design of fear conditioning tasks to assess individual differences and group-level inferences cross-sectionally and longitudinally.
100. 1. Ecology
     2. Plant Biology

     Rapid transgenerational adaptation in response to intercropping reduces competition

     Laura Stefan, Nadine Engbersen, Christian Schöb

     Annual crop communities are able to adapt towards reduced competition and/or increased facilitation in response to their neighboring diversity after only two generations.
101. 1. Epidemiology and Global Health
     2. Microbiology and Infectious Disease

     Effectiveness of rapid SARS-CoV-2 genome sequencing in supporting infection control for hospital-onset COVID-19 infection: Multicentre, prospective study

     Oliver Stirrup, James Blackstone ... Judith Breuer

     Sequencing of viral genomes could be useful for infection prevention and control within hospitals, but did not lead to a reduction in the rate of hospital-acquired SARS-CoV-2 infections over winter 2020/2021 in a large multicentre UK study.
102. 1. Neuroscience

     Neural effects of continuous theta-burst stimulation in macaque parietal neurons

     Maria C Romero, Lara Merken ... Marco Davare

     Electrophysiological recordings in awake behaving monkeys show the first evidence of the effect of continuous theta-burst stimulation, a widely used repetitive transcranial magnetic stimulation protocol, at the level of single neurons.
103. 1. Neuroscience

     Controllability boosts neural and cognitive signatures of changes-of-mind in uncertain environments

     Marion Rouault, Aurélien Weiss ... Valentin Wyart

     Task controllability manipulations reveal that information seeking is associated with reduced confidence and active hypothesis testing, as well as stronger neurophysiological correlates of attention and arousal.
104. 1. Developmental Biology

     Innexin function dictates the spatial relationship between distal somatic cells in the Caenorhabditis elegans gonad without impacting the germline stem cell pool

     Theadora Tolkin, Ariz Mohammad ... David Greenstein

     Somatic gonad architecture is regulated by innexin function, but sheath cell position does not determine germ cell exit from the stem cell pool.
105. 1. Evolutionary Biology

     Isometric spiracular scaling in scarab beetles—implications for diffusive and advective oxygen transport

     Julian M Wagner, C Jaco Klok ... Jon F Harrison

     Diffusion but not advection becomes increasingly challenging for gas transport across insect spiracles as body size increases.
106. 1. Cell Biology
     2. Structural Biology and Molecular Biophysics

     Structure of the HOPS tethering complex, a lysosomal membrane fusion machinery

     Dmitry Shvarev, Jannis Schoppe ... Christian Ungermann

     The structure of the yeast HOPS tethering complex suggests how this large complex may catalyze fusion by tethering Rab-decorated membranes and promoting the assembly of SNAREs.
107. 1. Microbiology and Infectious Disease

     Genetic and chemical validation of Plasmodium falciparum aminopeptidase PfA-M17 as a drug target in the hemoglobin digestion pathway

     Rebecca CS Edgar, Ghizal Siddiqui ... Tania F de Koning-Ward

     Plasmodium falciparum aminopeptidase PfA-M17 is essential to parasite survival and plays a role in hemoglobin digestion, providing a rationale for further development of inhibitors against this enzyme.
108. 1. Medicine

     A global view of the aspiring physician-scientist

     Christopher S Williams, W Kimryn Rathmell ... Mone Zaidi

     Challenges faced by the declining global workforce of young physician-scientists are highlighted, and a unified view on how to revive and reinvigorate this critical pool of dual-trained physicians worldwide is provided.
109. 1. Epidemiology and Global Health
     2. Microbiology and Infectious Disease

     Regional importation and asymmetric within-country spread of SARS-CoV-2 variants of concern in the Netherlands

     Alvin X Han, Eva Kozanli ... Chantal Reusken

     Flight restrictions targeted at countries where SARS-CoV-2 variant-of-concern first emerged have limited effectiveness in deterring their introduction into the Netherlands due to the strength of regional travel importation risks in Europe.
110. 1. Structural Biology and Molecular Biophysics

     Mars, a molecule archive suite for reproducible analysis and reporting of single-molecule properties from bioimages

     Nadia M Huisjes, Thomas M Retzer ... Karl E Duderstadt

     Mars provides a software platform for the development of complex single-molecule analysis workflows enabling the discovery and reporting of new biological phenomena with an open data format accessible to everyone.
111. 1. Computational and Systems Biology
     2. Evolutionary Biology

     Eco-evolutionary dynamics of clonal multicellular life cycles

     Vanessa Ress, Arne Traulsen, Yuriy Pichugin

     The evolution of clonal multicellular life cycles, whose growth is constrained by competition, may lead to coexistence or multistability between several life cycles while evolutionarily stable strategies can be inferred from the analysis of a model with unconstrained growth.
112. 1. Cell Biology
     2. Computational and Systems Biology

     A unified view of low complexity regions (LCRs) across species

     Byron Lee, Nima Jaberi-Lashkari, Eliezer Calo

     Disparate functions of low complexity regions of proteins can be understood through a global view of their sequences, features, and relationships across different organisms and biological contexts.
113. 1. Cancer Biology

     BRCA2 BRC missense variants disrupt RAD51-dependent DNA repair

     Judit Jimenez-Sainz, Joshua Mathew ... Ryan B Jensen

     Novel variants in the BRC repeat region of BRCA2 were identified and comprehensively characterized as either deleterious or benign.
114. 1. Neuroscience

     The role of anterior insular cortex inputs to dorsolateral striatum in binge alcohol drinking

     David L Haggerty, Braulio Munoz ... Brady K Atwood

     A novel brain circuit that determines how much alcohol a mouse binge drinks by complex control of drinking behavior mechanics is surveyed.
115. 1. Cell Biology
     2. Developmental Biology

     Live-cell imaging in human colonic monolayers reveals ERK waves limit the stem cell compartment to maintain epithelial homeostasis

     Kelvin W Pond, Julia M Morris ... Andrew L Paek

     Human colonic organoid monolayers self organize into regularly spaced stem and differentiated cell compartments, which are maintained by waves of ERK activity originating from extruded/dying cells.
116. 1. Neuroscience

     Neural synchronization is strongest to the spectral flux of slow music and depends on familiarity and beat salience

     Kristin Weineck, Olivia Xin Wen, Molly J Henry

     Two different analysis approaches for measuring neural synchronization to natural music revealed strongest synchronization to musical spectral flux as opposed to the more commonly used amplitude envelope.
117. 1. Biochemistry and Chemical Biology

     DNA passes through cohesin’s hinge as well as its Smc3–kleisin interface

     James E Collier, Kim A Nasmyth

     By identifying cohesin's DNA entry gate(s) and through understanding the corresponding topology between the two the mechanism behind cohesin's activity is slowly deciphered and will aid in understanding how the SMC family at large functions.
118. 1. Cell Biology

     The number of cytokinesis nodes in mitotic fission yeast scales with cell size

     Wasim A Sayyad, Thomas D Pollard

     Fission yeasts cells have about 190 cytokinesis nodes in a broad band at the equator which scales with cell size and most but not all nodes condense into the contractile ring.
119. 1. Developmental Biology
     2. Neuroscience

     Differentiation signals from glia are fine-tuned to set neuronal numbers during development

     Anadika R Prasad, Inês Lago-Baldaia ... Vilaiwan M Fernandes

     Extrinsic signals establish an invariant and stereotyped pattern of neuronal differentiation and programmed cell death in the Drosophila visual system.
120. 1. Neuroscience

     Time-resolved parameterization of aperiodic and periodic brain activity

     Luc Edward Wilson, Jason da Silva Castanheira, Sylvain Baillet

     The new method and reported findings address a growing interest in neuroscience for research tools that can reliably decompose brain activity at the mesoscopic scale into interpretable components.
121. 1. Structural Biology and Molecular Biophysics

     Unfolding and identification of membrane proteins in situ

     Nicola Galvanetto, Zhongjie Ye ... Vincent Torre

     The isolation of the cell membrane of single cells coupled with AFM enables the study and identification of native membrane proteins in a membrane fragment.
122. 1. Developmental Biology
     2. Stem Cells and Regenerative Medicine

     The C. elegans gonadal sheath Sh1 cells extend asymmetrically over a differentiating germ cell population in the proliferative zone

     Xin Li, Noor Singh ... Kacy Lynn Gordon

     As Caenorhabditis elegans adult hermaphrodite germ cells leave the stem cell niche, they associate with a pair of somatic gonad cells that were thought to be symmetrical, but that actually take on dramatically different positions in the organ.
123. 1. Microbiology and Infectious Disease

     The acid ceramidase/ceramide axis controls parasitemia in Plasmodium yoelii-infected mice by regulating erythropoiesis

     Anne Günther, Matthias Hose ... Wiebke Hansen

     Host cell acid ceramidase activity contributes to the regulation of erythrocyte maturation and thereby affects the frequency of target cells for rodent Plasmodium yoelii parasites.
124. 1. Neuroscience

     Foveal vision anticipates defining features of eye movement targets

     Lisa M Kroell, Martin Rolfs

     Before a saccadic eye movement to a location in the visual periphery, human observers' foveal vision becomes more sensitive to the features defining the eye movement target, anticipating information that will soon be fixated in a retinotopic reference frame.
125. 1. Computational and Systems Biology
     2. Neuroscience

     Automatically tracking feeding behavior in populations of foraging C. elegans

     Elsa Bonnard, Jun Liu ... Monika Scholz

     A new tool enables measuring feeding and locomotion simultaneously which will enable insights into environmental, developmental, neuronal, and genetic factors underlying behavioral regulation.
126. 1. Biochemistry and Chemical Biology
     2. Structural Biology and Molecular Biophysics

     Redox-controlled reorganization and flavin strain within the ribonucleotide reductase R2b–NrdI complex monitored by serial femtosecond crystallography

     Juliane John, Oskar Aurelius ... Martin Högbom

     The flavoprotein NrdI in class Ib ribonucleotide reductase controls superoxide generation and metal site oxidation by tuning the redox properties of its flavin cofactor via steric strain induced by the formation of the R2b–NrdI protein complex.
127. 1. Cancer Biology

     Arbidol inhibits human esophageal squamous cell carcinoma growth in vitro and in vivo through suppressing ataxia telangiectasia and Rad3-related protein kinase

     Ning Yang, Xuebo Lu ... Kangdong Liu

     Arbidol is a ATR inhibitor to reduce the phosphorylation and activation of MCM2 at Ser108, and innhibits the cell proliferation of ESCC in vitro and in vivo through the DNA replication and cell cycle pathway.
128. 1. Evolutionary Biology
     2. Genetics and Genomics

     GENESPACE tracks regions of interest and gene copy number variation across multiple genomes

     John T Lovell, Avinash Sreedasyam ... Jeremy Schmutz

     GENESPACE lets users track related chromosomal sequences across multiple reference genomes.
129. 1. Developmental Biology

     High hedgehog signaling is transduced by a multikinase-dependent switch controlling the apico-basal distribution of the GPCR smoothened

     Marina Gonçalves Antunes, Matthieu Sanial ... Isabelle Becam

     Hedgehog leads to Smoothened accumulation by favoring its recycling and high Hedgehog levels promote a basolateral enrichment of Smoothened via its phosphorylation by the protein kinase Fused.
130. 1. Stem Cells and Regenerative Medicine

     Injury-induced pulmonary tuft cells are heterogenous, arise independent of key Type 2 cytokines, and are dispensable for dysplastic repair

     Justinn Barr, Maria Elena Gentile ... Andrew E Vaughan

     Influenza-induced pulmonary tuft cells are a heterogenous population and emerge independently of Type 2 and interferon signaling, and do not impact dysplastic epithelial regeneration.
131. 1. Cancer Biology
     2. Immunology and Inflammation

     Cancer genome and tumor microenvironment: Reciprocal crosstalk shapes lung cancer plasticity

     Siavash Mansouri, Daniel Heylmann ... Rajkumar Savai

     The crosstalk between cancer cell genomic features and the tumor microenvironment (TME) to reveal the impact of genetic alterations on the TME phenotype.
132. 1. Neuroscience

     Low and high frequency intracranial neural signals match in the human associative cortex

     Corentin Jacques, Jacques Jonas ... Bruno Rossion

     Category-selective intracerebral neurophysiological activity in low- and high-frequency bands show unprecedented corresponding spatial, functional, and timing properties in the human brain.
133. 1. Microbiology and Infectious Disease

     Distinct regions of H. pylori’s bactofilin CcmA regulate protein–protein interactions to control helical cell shape

     Sophie R Sichel, Benjamin P Bratton, Nina R Salama

     The helical cell shape of Helicobacter pylori depends on the polymerizing cytoskeletal protein CcmA’s recruitment to the cell envelope by Csd5 and CcmA’s indirect stabilization of a periplasmic cell wall hydrolase via interactions with the transmembrane protein Csd7.
134. 1. Chromosomes and Gene Expression
     2. Structural Biology and Molecular Biophysics

     Fip1 is a multivalent interaction scaffold for processing factors in human mRNA 3′ end biogenesis

     Lena Maria Muckenfuss, Anabel Carmen Migenda Herranz ... Martin Jinek

     Molecular details of the interactions of human Fip1 with CPSF30 and CstF77 unravel key aspects of mRNA polyadenylation and its regulation.
135. 1. Neuroscience

     Evidence accumulation, not ‘self-control’, explains dorsolateral prefrontal activation during normative choice

     Cendri A Hutcherson, Anita Tusche

     A computational model of decision making suggests that the dorsolateral prefrontal cortex's role in self-control is more associated with evidence accumulation processes than with inhibition or modulation of value.
136. 1. Genetics and Genomics

     Integrative analysis of metabolite GWAS illuminates the molecular basis of pleiotropy and genetic correlation

     Courtney J Smith, Nasa Sinnott-Armstrong ... Jonathan K Pritchard

     Genetic variants acting between biologically related metabolite pairs often reflect biology local to the traits and have effect directions contrasting those of upstream or downstream variants, which can result in striking, biologically interpretable heterogeneity in genetic correlation across the genome.
137. 1. Cell Biology

     Regulation of pulmonary surfactant by the adhesion GPCR GPR116/ADGRF5 requires a tethered agonist-mediated activation mechanism

     James P Bridges, Caterina Safina ... Marie-Gabrielle Ludwig

     Mutagenesis and activity assays in vitro and in vivo identify residues in the tethered agonist of GPR116, as well as those within the extracellular loops, that are critical for mediating the response to the tethered agonist.
138. 1. Microbiology and Infectious Disease

     LINE-1 retrotransposons facilitate horizontal gene transfer into poxviruses

     M Julhasur Rahman, Sherry L Haller ... Stefan Rothenburg

     A new experimental system of horizontal gene transfer demonstrates that host retrotransposons facilitate the transfer of host genes into poxviruses.
139. 1. Neuroscience
     2. Structural Biology and Molecular Biophysics

     Fuzzy supertertiary interactions within PSD-95 enable ligand binding

     George L Hamilton, Nabanita Saikia ... Mark E Bowen

     A combination of single molecule fluorescence with DMD simulations and disulfide mapping resolved the multistate structural landscape of the PSG supramodule from PSD-95, which explains how interdomain interactions within PSD-95 enable PDZ3 binding of the critical synaptic adhesion protein neuroligin.
140. 1. Genetics and Genomics
     2. Stem Cells and Regenerative Medicine

     Highly efficient generation of isogenic pluripotent stem cell models using prime editing

     Hanqin Li, Oriol Busquets ... Frank Soldner

     A highly efficient platform to engineer designer mutations in human stem cells using RNA-based delivery of prime editing components.
141. 1. Computational and Systems Biology
     2. Plant Biology

     Computational modeling and quantitative physiology reveal central parameters for brassinosteroid-regulated early cell physiological processes linked to elongation growth of the Arabidopsis root

     Ruth Großeholz, Friederike Wanke ... Klaus Harter

     The recurring application of computational modelling combined with wet lab experiments identify and predict central regulatory parameters and factors for early cell physiological reactions linked to differential brassinosteroid-modified elongation growth in the root tip of Arabidopsis thaliana.
142. 1. Neuroscience

     Altered regulation of Ia afferent input during voluntary contraction in humans with spinal cord injury

     Bing Chen, Monica A Perez

     Physiological analysis reveals that during voluntary contraction Ia afferent input have a lesser facilitatory effect on motor neurons in humans with chronic incomplete spinal cord injury compared with control subjects.
143. 1. Cancer Biology

     Genetically manipulating endogenous Kras levels and oncogenic mutations in vivo influences tissue patterning of murine tumorigenesis

     Özgün Le Roux, Nicole LK Pershing ... Christopher M Counter

     Altering the level of different oncogenic mutants of Kras induces unique tumor patterns in mice, suggesting that tissue-specific responses mold the sensitivity of normal tissues to different oncogenic RAS mutations.
144. 1. Immunology and Inflammation

     Epigenetic remodeling by vitamin C potentiates plasma cell differentiation

     Heng-Yi Chen, Ana Almonte-Loya ... Chan-Wang Jerry Lio

     Vitamin C supports the differentiation of antibody-secreting cells by remodeling the DNA methylome to enhance STAT3 DNA binding.
145. 1. Neuroscience

     Impact of blindness onset on the representation of sound categories in occipital and temporal cortices

     Stefania Mattioni, Mohamed Rezk ... Olivier Collignon

     Early and late visual deprivation trigger a redeployment mechanism that reallocate part of the processing typically tagging the preserved senses (i.e. the temporal cortex for auditory stimulation) to the occipital cortex deprived of its most salient visual input.
146. 1. Evolutionary Biology
     2. Microbiology and Infectious Disease

     Poxviruses capture host genes by LINE-1 retrotransposition

     Sarah M Fixsen, Kelsey R Cone ... Nels C Elde

     Active selfish genetic elements in infected cells aid virus adaptation by catalyzing the transfer of host genes to virus genomes.
147. 1. Cell Biology
     2. Ecology

     Ecological lipidology

     Laura Christin Trautenberg, Marko Brankatschk ... Klaus Reinhardt

     Effects of dietary lipids on organismal function and reproductive fitness are persistent and may depend on lipid identity, cause the evolution of lipid-based diet choice, alter food webs, change species responses to environmental change, and affect human nutrition.
148. 1. Biochemistry and Chemical Biology
     2. Chromosomes and Gene Expression

     A specific role for importin-5 and NASP in the import and nuclear hand-off of monomeric H3

     Alonso Javier Pardal, Andrew James Bowman

     Histone H3 can translocate to the nucleus as a monomer through a pathway governed by importin-5 and transfers to the histone chaperone NASP, having implications in the folding of H3-H4 dimers and, therefore, the kinetics of genome packaging during replication.
149. 1. Neuroscience
     2. Structural Biology and Molecular Biophysics

     Proton-transporting heliorhodopsins from marine giant viruses

     Shoko Hososhima, Ritsu Mizutori ... Hideki Kandori

     A viral heliorhodopsin from Emiliania huxleyi virus 202 (V2HeR3) is a light-activated proton transporter, which has the potential to depolarize the host cells by light, possibly to overcome the host defense mechanisms or to prevent superinfection.
150. 1. Neuroscience
     2. Structural Biology and Molecular Biophysics

     Structural model of microtubule dynamics inhibition by kinesin-4 from the crystal structure of KLP-12 –tubulin complex

     Shinya Taguchi, Juri Nakano ... Ryo Nitta

     Structural, functional, and genetic analyses reveal how kinesin-4 motor KLP-12 precisely modulates the curvature of the microtubule plus-end to inhibit the microtubule dynamics for achieving the proper length control of axons.
151. 1. Biochemistry and Chemical Biology
     2. Physics of Living Systems

     Recovering mixtures of fast-diffusing states from short single-particle trajectories

     Alec Heckert, Liza Dahal ... Xavier Darzacq

     Tracking proteins in live cells is challenging due to technical limitations and biological complexity, but approaches based in Bayesian nonparametrics stand a decent chance at recovering a target protein's dynamic profile from noisy data.
152. 1. Cell Biology
     2. Immunology and Inflammation

     A crosstalk between hepcidin and IRE/IRP pathways controls ferroportin expression and determines serum iron levels in mice

     Edouard Charlebois, Carine Fillebeen ... Kostas Pantopoulos

     Under systemic iron overload, translational derepression of ferroportin mRNA via the IRE/IRP system antagonizes hepcidin-mediated ferroportin degradation to coordinately control serum iron.
153. 1. Neuroscience

     Machine learning-assisted fluoroscopy of bladder function in awake mice

     Helene De Bruyn, Nikky Corthout ... Thomas Voets

     A new combined approach using X-ray fluoroscopy and machine learning provides detailed new insights into bladder function in awake, unrestrained mice and unveils important limitations of current approaches to study bladder function.
154. 1. Neuroscience

     Molecular characteristics and laminar distribution of prefrontal neurons projecting to the mesolimbic system

     Ákos Babiczky, Ferenc Matyas

     Molecular, laminar, and regional characterization in combination with classical and conditional tracing techniques reveal that medial prefrontal cortical neurons innervating the nucleus accumbens and the ventral tegmental area form distinct populations.
155. 1. Biochemistry and Chemical Biology
     2. Structural Biology and Molecular Biophysics

     Structure of Geobacter OmcZ filaments suggests extracellular cytochrome polymers evolved independently multiple times

     Fengbin Wang, Chi Ho Chan ... Daniel R Bond

     A cryo-EM structure of the bacterial OmcZ cytochrome filament provides evidence for the possibility that cytochrome polymers had multiple origins in bacteria.
156. 1. Biochemistry and Chemical Biology
     2. Cell Biology

     Multiple ciliary localization signals control INPP5E ciliary targeting

     Dario Cilleros-Rodriguez, Raquel Martin-Morales ... Francesc R Garcia-Gonzalo

     Four conserved ciliary localization signals in the Joubert syndrome-associated INPP5E phosphoinositide phosphatase control its ciliary accumulation.
157. 1. Neuroscience

     Amygdala-cortical collaboration in reward learning and decision making

     Kate M Wassum

     A review of a neuronal circuit architecture that helps us to learn about rewarding events and then to use that information to support adaptive decision making.
158. 1. Biochemistry and Chemical Biology
     2. Cell Biology

     Distinct architectural requirements for the parS centromeric sequence of the pSM19035 plasmid partition machinery

     Andrea Volante, Juan Carlos Alonso, Kiyoshi Mizuuchi

     Unique sequence feature requirements for the plasmid centromere pSM19035-parS for ParA_pSM-ATPase activation byribbon-helix-helix-ParB_pSM, a non-CTPase centromere-binding protein, evince complex interaction gymnastics among the three reaction components necessary for plasmid partition, which is distinct from ParABS-systems involving helix-turn-helix-ParB-CTPases.
159. 1. Neuroscience

     Deep learning-based feature extraction for prediction and interpretation of sharp-wave ripples in the rodent hippocampus

     Andrea Navas-Olive, Rodrigo Amaducci ... Liset M de la Prida

     A new method is described to identify sharp-wave ripples from the rodent hippocampus with deep learning techniques, which may help to identify and characterize previously undetected physiological events.
160. 1. Microbiology and Infectious Disease

     Hemocyte differentiation to the megacyte lineage enhances mosquito immunity against Plasmodium

     Ana Beatriz F Barletta, Banhisikha Saha ... Carolina Barillas-Mury

     Toll signaling modulates hemocyte differentiation into the megacyte lineage and their recruitment to the midgut greatly enhances mosquito immunity against Plasmodium.
161. 1. Medicine
     2. Neuroscience

     Stage-dependent differential influence of metabolic and structural networks on memory across Alzheimer’s disease continuum

     Kok Pin Ng, Xing Qian ... Alzheimer's Disease Neuroimaging Initiative

     The early influence of the breakdown of both hippocampal structural network and angular gyrus-seeded default mode metabolism network on memory performance underscore the importance of early intervention in preclinical Alzheimer’s disease.
162. 1. Cell Biology
     2. Neuroscience

     Keratinocyte PIEZO1 modulates cutaneous mechanosensation

     Alexander R Mikesell, Olena Isaeva ... Cheryl L Stucky

     Epidermal PIEZO1 is a key keratinocyte mechanotransducer which mediates normal behavioral and sensory neuron responses to cutaneous mechanical stimulation.
163. 1. Biochemistry and Chemical Biology
     2. Cancer Biology

     Crosstalk between AML and stromal cells triggers acetate secretion through the metabolic rewiring of stromal cells

     Nuria Vilaplana-Lopera, Vincent Cuminetti ... Paloma Garcia

     Interplay between AML and stromal cells can initiate a mechanism involving gap junctions where ROS is transferred from cancer cells to stromal cells which then produce acetate which can in return be absorbed/utilised by the cancer cells.
164. 1. Genetics and Genomics

     Data-driven, participatory characterization of farmer varieties discloses teff breeding potential under current and future climates

     Aemiro Bezabih Woldeyohannes, Sessen Daniel Iohannes ... Matteo Dell'Acqua

     A genomic, climatic, agronomic, and participatory characterization of teff agrobiodiversity shows opportunities and challenges of teff improvement in the face of climate change.
165. 1. Microbiology and Infectious Disease

     Vaccination decreases the risk of influenza A virus reassortment but not genetic variation in pigs

     Chong Li, Marie R Culhane ... Montserrat Torremorell

     Vaccination has the potential to decrease swine influenza diversification by restricting influenza virus co-infections and reassortment events in pigs.
166. 1. Cell Biology

     Viscoelastic properties of suspended cells measured with shear flow deformation cytometry

     Richard Gerum, Elham Mirzahossein ... Ben Fabry

     Under fluid shear, living cells deform elliptically, align in flow direction, and rotate, from which their frequency-dependent viscoelastic properties can be inferred.
167. 1. Medicine

     Brain atlas for glycoprotein hormone receptors at single-transcript level

     Vitaly Ryu, Anisa Gumerova ... Mone Zaidi

     The most comprehensive neuroanatomical atlas on the expression of three glycoprotein hormone receptors, namely, TSHRs, LHCGRs, and FSHRs, was mapped using RNAscope, a technology that allows the detection of mRNA at single-transcript level.
168. 1. Cell Biology

     Ait1 regulates TORC1 signaling and localization in budding yeast

     Ryan L Wallace, Eric Lu ... Andrew P Capaldi

     The budding yeast gained a novel regulator of TORC1 signaling, called Ait1, 150–200 million years ago, around the same time they lost functional Rheb and Tsc1/2.
169. 1. Evolutionary Biology
     2. Genetics and Genomics

     Most cancers carry a substantial deleterious load due to Hill-Robertson interference

     Susanne Tilk, Svyatoslav Tkachenko ... Christopher D McFarland

     The absence of negative selection observed in most cancer genomes can be explained by the intrinsic genome-wide linkage in somatic evolution and creates a substantial proteotoxic load.
170. 1. Biochemistry and Chemical Biology

     Specific binding of Hsp27 and phosphorylated Tau mitigates abnormal Tau aggregation-induced pathology

     Shengnan Zhang, Yi Zhu ... Dan Li

     Structural basis and molecular mechanism of how molecular chaperone Hsp27 specifically captures phosphorylated Tau and prevents it from abnormal aggregation.
171. 1. Biochemistry and Chemical Biology
     2. Evolutionary Biology

     Comprehensive phylogenetic analysis of the ribonucleotide reductase family reveals an ancestral clade

     Audrey A Burnim, Matthew A Spence ... Nozomi Ando

     A large-scale phylogenetic inference of the ribonucleotide reductase family reveals a new distinct clade with implications on how nature adapted to environmental changes.
172. 1. Cell Biology
     2. Neuroscience

     Chronic neurotransmission increases the susceptibility of lateral-line hair cells to ototoxic insults

     Daria Lukasz, Alisha Beirl, Katie Kindt

     In vivo imaging reveals that the synaptic vesicle cycle is a significant contributor to oxidative stress in sensory hair cells, rendering them susceptible to insults.
173. 1. Plant Biology

     Uncovering natural variation in root system architecture and growth dynamics using a robotics-assisted phenomics platform

     Therese LaRue, Heike Lindner ... José R Dinneny

     Advances in robotics-assisted plant handling and automated image analysis enables the characterization of root growth dynamics for Arabidopsis accessions at late stages of development and the association of these traits with genetic variation and climatic variables.
174. 1. Cell Biology

     The skeletal muscle circadian clock regulates titin splicing through RBM20

     Lance A Riley, Xiping Zhang ... Karyn A Esser

     The muscle circadian clock regulates RNA-binding motif 20 (Rbm20) gene expression, providing a novel mechanism for titin splicing and isoform expression with implications for muscle structure and function.