Paul Richard J Yulo, Nicolas Desprat ... Heather L Hendrickson
Fitness defects arising from deletion of the gene encoding the bacterial equivalent of actin (mreB) are readily compensated by mutations affecting cell wall synthesis.
Ainhoa Arbués, Sarah Schmidiger ... Damien Portevin
Genetically diverse Mycobacterium tuberculosis complex strains induce a gamut of human granulomatous responses in vitro, with a role for IL-1β in aggregate formation.
Eva Herdering, Tristan Reif-Trauttmansdorff ... Ruth Anne Schmitz
The central nitrogen-metabolite 2-oxoglutarate directly promotes glutamine synthetase activity by inducing its dodecameric assembly and priming the active sites, representing a novel mechanism of regulation.
Jianheng Fox Liu, Ben R Hawley ... Samie R Jaffrey
CROWN-seq provides the first precise and quantitative map of N6,2’-O-dimethyladenosine (m6Am) in mRNA and snRNA, revealing the link between m6Am modification and efficient transcription, rather than translation and RNA stability.
Kathleen T Quach, Gillian A Hughes, Sreekanth H Chalasani
As Caenorhabditis elegans shifts from reactive to increasingly proactive defensive strategies, nuanced changes in interaction between SEB-3 receptor and NLP-49 peptides regulate the transitions between defensive behaviors.
Maryam Rahmati Ishka, Hayley Sussman ... Magdalena M Julkowska
Salt stress disrupts root-shoot growth coordination through SR3G, a negative regulator of salt tolerance, that affects shoot growth, root suberization, and sodium accumulation, revealing new targets for environmental resilience.
A novel mutant, R25CPTH, derived from a hypoparathyroidism patient, can form a dimer that activates PTH1R and enhances bone formation in female mice similar to PTH despite altered molecular properties.
Cortex-wide projections onto primary sensory and motor areas display substantial interhemispheric symmetry in their areal input identity and are primarily feedback in nature due to dominant inputs from Layer 6.
Sujeethkumar Prithiviraj, Alejandro Garcia Garcia ... Paul E Bourgine
Human extracellular matrices can be edited in their composition using the CRISPR/Cas9 system, leading to materials exhibiting tailored regenerative capacities.
Multi-omics analysis shows that preeclampsia-specific immune cell network was regulated by Folr2+Ccl7+Ccl8+C1qa+C1qb+C1qc+ pro-inflammatory macrophages, which can induce the CD8+ and Th17 memory T cells generation while inhibiting the gMDSCs production.
Elissa Sutlief, Charlie Walters ... Marshall G Hussain Shuler
Equations generalizing reward rate maximization are derived, explaining ostensible suboptimal behaviors, revealing time’s cost to comprise apportionment and opportunity costs, and identifying the misestimation in time apportionment as the actual error committed in temporal decision making by animals and humans.
It was revealed that phosphorylation of the DNA repair enzyme PNKP at threonine 118 by CDK is required for DNA replication through the gap filling of Okazaki fragments.
Analysis of inflammasome responses in human macrophages reveals that inflammatory caspases and pyroptotic factors control Salmonella replication primarily within the cytosol and also within vacuoles.
Daniel Patrick Gainey, Andrey V Shubin, Craig P Hunter
The failure to replicate key findings from an important report on transgenerational inheritance in Caenorhabditis elegans raises concerns about the robustness and ecological significance of the previously reported results.
Assmaa Elsheikh, Camden M Driggers ... Show-Ling Shyng
Structure-based approach identifies a KATP-binding compound that has the potential of normalizing insulin secretion in congenital hyperinsulinism by correcting defective KATP channel trafficking to the cell surface.
The microenvironment within biomolecular condensates exhibits interfacial behaviors characterized by a combination of hydrophobic and hydrophilic properties.
Vladimir Khayenko, Cihan Makbul ... Hans Michael Maric
Targeting two novel sites on the hepatitis B virus capsid disrupts its assembly and induces aggregation, suggesting potential for antiviral therapy development.
Olga Kepinska, Josue Dalboni da Rocha ... Narly Golestani
Multilingual experience, particularly with larger phonological inventories across multilinguals’ languages, is linked to structural differences in transverse temporal gyri, highlighting experience-driven plasticity as a key factor shaping auditory cortex anatomy.
Pierluigi Scerbo, Benjamin Tisserand ... Bertrand Ducos
Oncogene permanent activation in a single targeted cell of zebrafish together with a reprogramming factor transient activation is sufficient to induce the malignant transformation of the cell and tumor development.
Oxidized LDL synergizes with angiotensin II to amplify Gq signaling via the AT1-LOX1 receptor complex, revealing a novel mechanism driving renal dysfunction in hypertension and dyslipidemia.
Gabriel Magno de Freitas Almeida, Iker Arriaga ... Lotta-Riina Sundberg
Jyvaskylavirus, the northernmost isolate of the Marseilleviridae family, shows that giant viruses are also part of the boreal Finnish environment and brings structural information with relevance for understanding other viruses.
Mice lacking the secreted hormone, CTRP10, represent a unique female model of obesity with preserved metabolic health, highlighting sex-specific mechanisms that uncouple obesity from metabolic dysfunction.
Hippocampal replay, which is implicated in memory formation and planning, exhibits a reward-dependent spatial specificity that in novel environments requires dopamine signaling.
ORMDL3 promotes RIG-I degradation to suppress type I interferon signaling pathway and antiviral immune response, highlighting ORMDL3 as a potential therapeutic target in viral infection and cancer immunotherapy.
A multiomic study reveals the role of ZFHX3 transcription factor in daily timekeeping with a combination of transcription factor and histone ChIP-seq and time-resolved RNA-seq in the mammalian central clock.
Neurobehavioral data combined with computational models shows the superiority of active inference models in explaining human decisions under uncertainty.
Gülnihal Kavaklioglu, Alexandra Podhornik ... Christian Seiser
DNA hypomethylation in human tumor cells results in the activation of the LINE-1-derived RNA-binding protein L1TD1 and its interactions with LINE-1 transposons.
Multimodal imaging reveals overlapping gradients of hippocampal connectivity reflecting patterns of cortical function, dopamine receptor distribution, and individual differences in memory, with age-related dedifferentiation of gradient organization marking cognitive decline.
ATP6V0A1 was identified as a key player in nephropathic cystinosis-related renal pathology, with antioxidants like ATX emerging as potential treatments to restore V0A1 expression, mitochondrial function, and balance autophagic processes.
Genetic diversity is strongly associated with ecosystem functions across natural trophic chains, showing that it can be as important as species diversity.
V2 exhibits interdigitated columnar organizations for color and disparity that involved feedforward and feedback processing, while the texture selectivity is primarily driven by feedback modulations from V4.
Torsten Günther, Jacob Chisausky ... Cristina Valdiosera
After their introduction to the Iberian peninsula, domestic cattle hybridised with mostly male wild aurochs for millennia until human actions likely ended this process about 4000 years ago.
Pooja Popli, Arin K Oestreich ... Ramakrishna Kommagani
Autophagy sustains oviduct homeostasis, modulates inflammation, and prevents excessive pyroptosis, ensuring efficient embryo transport and a well-balanced reproductive tract environment.
A Hebbian learning rule accurately predicts the changes in synaptic organization induced by retinal axons that grow to the wrong position in the visual thalamus.
Pooja Popli, Arin K Oestreich ... Ramakrishna Kommagani
Autophagy sustains oviduct homeostasis, modulates inflammation, and prevents excessive pyroptosis, ensuring efficient embryo transport and a well-balanced reproductive tract environment.
Human electroencephalography reveals that neural speech tracking is enhanced by minimal background noise and that this enhancement is independent of attention and generalizes across noise types and sound-delivery systems.
Jasper Janssens, Pierre Mangeol ... Frank Schnorrer
Spatial transcriptomics of adult Drosophila brain and body sections determined the spatial expression for 150 genes, which identified the locations of unknown neuronal cell types and transcriptional diversity within muscle.
Imaging and correlative light and electron microscopy analyses of endosomal-lysosomal organellar assembly in mammalian oocytes elucidated statistic alteration in their size, distribution, and correlation with endo-lysosomal maturation during oocyte-to-embryo transition.
BMD mice with three different in-frame deletions corresponded to the severity of BMD patients with the same deletions, and they showed type IIa muscle fibers reduction with morphological capillary changes.
A differentiable variant of the Gillespie algorithm enables gradient-based optimization for stochastic chemical kinetics, facilitating efficient parameter estimation and the design of biochemical networks with desired input–output relationships.
Implicitly learned spatial priors shape early visual cortex responses by suppressing potential distractions before stimuli appear, revealing a proactive mechanism that may enhance attentional control.
The term 'parasympathetic', unlike the term 'sympathetic', unduly unifies an assemblage of autonomic pathways which are unrelated by anatomy, physiology or genetics, and whose distinction can foster a better understanding of the autonomic nervous system.
The cell membrane receptors of juvenile hormone, CAD96CA and FGFR1, regulate the genomic effects of juvenile hormone by phosphorylating intracellular receptor.
A novel mouse model offers definitive evidence that a single inhibitory receptor family governs the critical natural killer cell functions of licensing and missing-self.
Foxg1 regulates gliogenesis in the mouse neocortex by modulating FGF signalling and integrating intrinsic and extrinsic mechanisms to ensure a balanced production of neurons, astrocytes, and oligodendrocytes in the brain.
Differentiation of human pluripotent stem cells mutant for one GATA6 gene can model developmental heart defects seen in some patients with congenital heart disease.
RNA interference pathways are spatially and temporally organized during Caenorhabditis elegans embryogenesis, with the Argonaute protein NRDE-3 playing distinct roles in regulating genes in different cell types and developmental stages.
The gut microbiome regulates protein absorption activity in lysosome-rich enterocytes (LREs) and causes broad gene expression changes, while LRE activity also affects the microbiome.
Nelson V Simwela, Eleni Jaecklein ... David G Russell
Prevention of either lipid uptake or lipid catabolism in infected macrophages restricts the ability of Mycobacterium tuberculosis to grow inside these cells.
Using single-cell RNA sequencing, we mapped thousands of expression quantitative trait loci in yeast, including a variant in GPA1 that influences gene expression, cell-cycle occupancy, and mating efficiency.
Gabriel Loewinger, Erjia Cui ... Francisco Pereira
A fiber photometry analysis framework based on functional mixed models enhances the detection of effects by testing signal-variable associations at each trial timepoint and accounting for between-animal heterogeneity.
Gene duplication in antibiotic-resistant bacteria mitigates elevated demand for expression, facilitating adaptation to antibiotics and alleviating costs of proteolytic instability of the drug target.
Congenital deafness increases individual differences in brain connectivity from the auditory cortex, particularly to language-related regions, highlighting how sensory loss shapes brain organization and development and the importance of personalized rehabilitation.
Barbora Rehak Buckova, Charlotte Fraza ... Jaroslav Hlinka
Normative modelling adapted for longitudinal neuroimaging enables precise evaluation of individual brain changes over time, revealing subtle disease dynamics and temporal patterns missed by traditional analytical approaches.
Thomas A Bos, Elizaveta Polyakova ... Monique RM Jongbloed
Although methods to efficiently generate functional autonomic postganglionic neurons from human pluripotent stem cells largely mirror signaling cues during embryonic development, several in vivo signaling cues remain to be explored in vitro.
Genome-wide CRISPR screen identified propionyl-CoA subunit B as a key regulator for anti-tumor T-cell activity in pancreatic cancer, which holds promise for advancing immunotherapy strategies in curing pancreatic cancer.
Matthew A Churgin, Danylo O Lavrentovich ... Benjamin L de Bivort
A fly’s preference for one odor versus another can be predicted by the idiosyncratic activity of neurons early on in the olfactory information processing circuit.
DLK signaling network reveals the conserved function in neuritogenesis and synapse formation, and highlights the regulation of c-Jun translation and microtubule homeostasis in hippocampal selective neuron vulnerability.
Derived from scRNA-seq, SLC26A3 is a potential therapeutic target to improve the efficacy of immunotherapy, as well as a potential diagnostic indicator to reduce post-surgical upstaging, for cervical adenocarcinoma.
Adriana K Alexander, Karina F Rodriguez ... Humphrey HC Yao
Single-cell multiomic analyses reveal known and new genes and pathways involved in the development of germ cells and provide a discovery platform for the scientific community.
The combination of receptor expression analysis, optogenetics, physiological approaches, and connectomics reveals how a heterogeneous population of insulin-producing cells in Drosophila integrates diverse modulatory inputs to adjust insulin release.
Tanja Fuchsberger, Imogen Stockwell ... Ole Paulsen
The reward signal dopamine modulates synaptic plasticity by increasing protein synthesis, revealing a potential molecular mechanism for long-term memory storage.
Structural and functional analyses identify a new family of bifunctional cell-wall-targeting type VI secretion system (T6SS) effectors and a surface-charge-engineering strategy that expands T6SS activity against Gram-positive cells.
Healthy lifestyle changes, particularly improvements in diet and smoking, were inversely related to comprehensive and organ-specific biological aging in Southwest China, highlighting key intervention targets to limit aging.
The cytological mechanism of conjugated linoleic acids regulates fat infiltration in skeletal muscles based on pig models via using single-nucleus transcriptomics.
Recurrent spiking networks that process input stimuli with optimal efficiency have key emerging properties that are similar to those of biological neural networks.
Sergio Casas-Tinto, Nuria Garcia-Guillen, María Losada-Perez
Genetic and lineage-tracing analyses reveal that neuropil-associated glia can directly transdifferentiate into neurons in the adultcentral nervous system (CNS) after injury, uncovering a previously unrecognised mechanism for CNS regeneration.
Mihály Vöröslakos, Yunchang Zhang ... György Buzsáki
The ThermoMaze behavioral paradigm enables the collection of extensive physiological data while animals remain at distinct experimenter-controlled locations during rest.
The intrinsic factor of cell senescence, PCBP2, was identified by unlabeled quantitative proteomics, and its biological role in in vitro replication senescence of human bone marrow mesenchymal stromal cells was characterized in vitro.
Multi-dimensional social relationships dynamically shape attentional biases toward in-group and out-group conspecifics in monkeys, with oxytocin modulating these interactions to reveal neurobiological links between social networks and visual information processing.
Zuzana Outla, Gizem Oyman-Eyrilmez ... Martin Gregor
Plectin drives hepatocellular carcinoma progression and metastasis through cytoskeletal reorganization and oncogenic signaling, making it a promising therapeutic target for overcoming therapy resistance.
Assessment of transfer entropy is useful for network analysis of cell signaling network providing information on the network topology and action points of genetic mutations and drug effect, thus will be applicable to molecular pharmacology and pathology.
Alison G Barber, Cynthia M Quintero ... Tannishtha Reya
The stem cell signal Msi2 plays a dual role in lung cancer, acting as a signal that is not only essential for initiation, but also for propagation of the tumor.
Diellor Basha, Amirmohammad Azarmehri ... Igor Timofeev
The nucleus reuniens of the thalamus play a central role in synchronizing the mPFC-hippocampal network during sleep and integrates converging activity from hippocampal and mPFC oscillations.
Cameron T Ellis, Tristan S Yates ... Nicholas Turk-Browne
The visual system of infants has adult-like properties, and these properties can be revealed at an individual level by having infants watch movies during functional magnetic resonance imaging.
Flavia A Zanetti, Ignacio Fernandez ... Laura Ruth Delgui
Molecular, biophysical, and computational analysis reveals insights on the mechanism of association of birnavirus VP3 with endosomes and its role in the viral life cycle, representing a substantial contribution toward understanding the replication strategy of these 'non-canonical' viruses.
Kayleigh Bozon, Hartmut Cuny ... Sally L Dunwoodie
NAD de novo synthesis activity in the murine visceral yolk sac is essential for embryonic development and its perturbation due to genetic and maternal dietary factors causes NAD deficiency and congenital malformations.
Olivia B Taylor, Nicholas DeGroff ... Andy J Fischer
Inflammatory S1P signaling is dynamically regulated in chick Müller glia after retinal injury and suppresses Müller glia reprogramming into neuron-like cells.
Tissue-specific transcription factors, such as basic motif leucine zipper protein NRL, interact with a plethora of RNA-binding proteins to perform pleiotropic functions during distinct steps of transcription process to generate quantitatively precise patterns of gene expression in neuronal cells.
Kentaro K Ishii, Koichi Hashikawa ... Garret D Stuber
Inhibitory neurons in the female medial preoptic area show persistent activity in response to male ejaculation, and their reactivation is sufficient to suppress female sexual motivation.
Root causal genes correspond to the initial changes to gene expression that induce a diagnosis and can be reliably detected from bulk RNA-seq after determining a causal ordering from Perturb-seq.
Philipp S O'Neill, Martín Baccino-Calace ... Igor Delvendahl
miniML, a deep learning-based method, facilitates synaptic event analysis with high accuracy and versatility across diverse synaptic preparations, enabling high-throughput investigations of neural function and dysfunction.
Maxine K Loh, Samantha J Hurh ... Mitchell F Roitman
Phasic dopamine cell body activity and release in the nucleus accumbens differentially respond to the taste of sucrose in correlation with its hedonic valuation.
A non-apoptotic function of the caspase Dronc is essential to promote tissue repair in response to necrosis via a previously uncharacterized mechanism.
It is safe to transplant autologous P63+ progenitor cells isolated from healthy bronchi to IPF patients for the treatment of IPF, and preliminary efficacy has been demonstrated in some patients.
Newly produced genomic data, as well as available datasets, reveal the pathogen's global transmission history along with the live poultry trade and pinpoint mobile element-driven local Salmonella adaptation.
Rui Vasco Simoes, Rafael Neto Henriques ... Noam Shemesh
Deuterium metabolic imaging quantitatively maps glycolytic and mitochondrial oxidation fluxes in mouse glioblastoma and their association with tumor histopathologic features.
Transmissible tumors in Hydra can manipulate host phenotype by inducing the growth of supernumerary tentacles, enhancing host foraging efficiency and promoting tumor vertical transmission, akin to manipulative parasites.
Attenuation of viral Nef-driven PI3 kinase signaling in B cells precedes the induction of neutralizing antibody responses against a difficult-to-neutralize simian immunodeficiency virus.
Prostaglandin E2 inhibits Kv2.2 channels via the EP2/4-PKA signaling pathway, leading to decreased glucose-stimulated action potential firing and insulin secretion in normal pancreatic β-cells.
Mathematical models of horizontal gene transfer reveal the determinants underlying multistability of microbial communities, offering key insights for the predictive control and engineering of complex microbiota.
A combination of polymer physics theories consistently rationalizes comprehensive experimental effects of salt, ATP, phosphorylation, and sequence charge and arginine/lysine patterns on the reentrant phase behaviors of intrinsically disordered proteins.
Agnieszka Glica, Katarzyna Wasilewska ... Katarzyna Jednoróg
Findings challenge the neural noise hypothesis of dyslexia, showing no differences in excitatory/inhibitory balance between individuals with dyslexia and controls.
Cristina Paissoni, Sarita Puri ... Carlo Camilloni
Computational modeling and in vitro experiments identify a unique conformational state in amyloidogenic light chains, providing a structural basis for amyloidosis and a precise target for drug design.
David C Williams, Amanda Chu ... Michael A McDannald
A more complete, bidirectional relationship between fear conditioning and behavior is established when many behaviors are examined, as opposed to single behavior such as freezing.
